Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14960 and AMB_RS14965 are separated by 67 nucleotides AMB_RS14965 and AMB_RS24565 overlap by 4 nucleotides
AMB_RS14960: AMB_RS14960 - hypothetical protein, at 3,204,569 to 3,205,738
_RS14960
AMB_RS14965: AMB_RS14965 - DUF2339 domain-containing protein, at 3,205,806 to 3,208,412
_RS14965
AMB_RS24565: AMB_RS24565 - hypothetical protein, at 3,208,409 to 3,209,656
_RS24565
Position (kb)
3205
3206
3207
3208
3209 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3204.842 kb on + strand, within AMB_RS14960 at 3204.843 kb on - strand, within AMB_RS14960 at 3204.854 kb on + strand, within AMB_RS14960 at 3204.855 kb on - strand, within AMB_RS14960 at 3204.855 kb on - strand, within AMB_RS14960 at 3204.872 kb on + strand, within AMB_RS14960 at 3204.872 kb on + strand, within AMB_RS14960 at 3204.873 kb on - strand, within AMB_RS14960 at 3204.873 kb on - strand, within AMB_RS14960 at 3204.873 kb on - strand, within AMB_RS14960 at 3204.873 kb on - strand, within AMB_RS14960 at 3204.891 kb on - strand, within AMB_RS14960 at 3204.956 kb on + strand, within AMB_RS14960 at 3204.956 kb on + strand, within AMB_RS14960 at 3204.956 kb on + strand, within AMB_RS14960 at 3204.957 kb on - strand, within AMB_RS14960 at 3204.957 kb on - strand, within AMB_RS14960 at 3205.079 kb on + strand, within AMB_RS14960 at 3205.079 kb on + strand, within AMB_RS14960 at 3205.080 kb on - strand, within AMB_RS14960 at 3205.080 kb on - strand, within AMB_RS14960 at 3205.080 kb on - strand, within AMB_RS14960 at 3205.274 kb on + strand, within AMB_RS14960 at 3205.405 kb on + strand, within AMB_RS14960 at 3205.405 kb on + strand, within AMB_RS14960 at 3205.405 kb on + strand, within AMB_RS14960 at 3205.405 kb on + strand, within AMB_RS14960 at 3205.406 kb on - strand, within AMB_RS14960 at 3206.361 kb on + strand, within AMB_RS14965 at 3206.361 kb on + strand, within AMB_RS14965 at 3206.370 kb on + strand, within AMB_RS14965 at 3206.370 kb on + strand, within AMB_RS14965 at 3206.371 kb on - strand, within AMB_RS14965 at 3206.371 kb on - strand, within AMB_RS14965 at 3206.950 kb on - strand, within AMB_RS14965 at 3206.950 kb on - strand, within AMB_RS14965 at 3207.195 kb on + strand, within AMB_RS14965 at 3207.196 kb on - strand, within AMB_RS14965 at 3207.525 kb on + strand, within AMB_RS14965 at 3207.525 kb on + strand, within AMB_RS14965 at 3207.525 kb on + strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.526 kb on - strand, within AMB_RS14965 at 3207.537 kb on + strand, within AMB_RS14965 at 3207.750 kb on + strand, within AMB_RS14965 at 3207.884 kb on - strand, within AMB_RS14965 at 3207.999 kb on + strand, within AMB_RS14965 at 3207.999 kb on + strand, within AMB_RS14965 at 3208.167 kb on + strand at 3208.167 kb on + strand at 3208.374 kb on + strand at 3208.897 kb on + strand, within AMB_RS24565 at 3208.898 kb on - strand, within AMB_RS24565 at 3208.993 kb on + strand, within AMB_RS24565 at 3209.015 kb on - strand, within AMB_RS24565 at 3209.015 kb on - strand, within AMB_RS24565
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 3,204,842 + AMB_RS14960 0.23 -3.4 3,204,843 - AMB_RS14960 0.23 -0.1 3,204,854 + AMB_RS14960 0.24 -1.7 3,204,855 - AMB_RS14960 0.24 -1.7 3,204,855 - AMB_RS14960 0.24 +0.6 3,204,872 + AMB_RS14960 0.26 +0.2 3,204,872 + AMB_RS14960 0.26 +0.2 3,204,873 - AMB_RS14960 0.26 +0.7 3,204,873 - AMB_RS14960 0.26 -0.4 3,204,873 - AMB_RS14960 0.26 +0.5 3,204,873 - AMB_RS14960 0.26 -1.1 3,204,891 - AMB_RS14960 0.28 +0.2 3,204,956 + AMB_RS14960 0.33 -0.2 3,204,956 + AMB_RS14960 0.33 +0.3 3,204,956 + AMB_RS14960 0.33 -0.1 3,204,957 - AMB_RS14960 0.33 -1.7 3,204,957 - AMB_RS14960 0.33 -0.5 3,205,079 + AMB_RS14960 0.44 -1.1 3,205,079 + AMB_RS14960 0.44 -1.4 3,205,080 - AMB_RS14960 0.44 -2.2 3,205,080 - AMB_RS14960 0.44 +0.3 3,205,080 - AMB_RS14960 0.44 -0.7 3,205,274 + AMB_RS14960 0.60 -1.3 3,205,405 + AMB_RS14960 0.71 +2.6 3,205,405 + AMB_RS14960 0.71 -2.0 3,205,405 + AMB_RS14960 0.71 -2.4 3,205,405 + AMB_RS14960 0.71 -2.7 3,205,406 - AMB_RS14960 0.72 -1.1 3,206,361 + AMB_RS14965 0.21 -0.5 3,206,361 + AMB_RS14965 0.21 +0.2 3,206,370 + AMB_RS14965 0.22 -0.5 3,206,370 + AMB_RS14965 0.22 +0.1 3,206,371 - AMB_RS14965 0.22 -0.2 3,206,371 - AMB_RS14965 0.22 -0.5 3,206,950 - AMB_RS14965 0.44 -1.8 3,206,950 - AMB_RS14965 0.44 +0.8 3,207,195 + AMB_RS14965 0.53 +0.0 3,207,196 - AMB_RS14965 0.53 +0.8 3,207,525 + AMB_RS14965 0.66 +1.5 3,207,525 + AMB_RS14965 0.66 -1.3 3,207,525 + AMB_RS14965 0.66 -0.1 3,207,526 - AMB_RS14965 0.66 +0.3 3,207,526 - AMB_RS14965 0.66 -0.2 3,207,526 - AMB_RS14965 0.66 +0.5 3,207,526 - AMB_RS14965 0.66 -2.2 3,207,526 - AMB_RS14965 0.66 -1.3 3,207,526 - AMB_RS14965 0.66 -4.5 3,207,537 + AMB_RS14965 0.66 +0.6 3,207,750 + AMB_RS14965 0.75 +0.4 3,207,884 - AMB_RS14965 0.80 +0.0 3,207,999 + AMB_RS14965 0.84 +0.3 3,207,999 + AMB_RS14965 0.84 -1.3 3,208,167 + -1.7 3,208,167 + +0.1 3,208,374 + -0.2 3,208,897 + AMB_RS24565 0.39 -0.1 3,208,898 - AMB_RS24565 0.39 -0.5 3,208,993 + AMB_RS24565 0.47 +0.2 3,209,015 - AMB_RS24565 0.49 +0.3 3,209,015 - AMB_RS24565 0.49 +0.2
Or see this region's nucleotide sequence