Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14910 and AMB_RS14915 are separated by 15 nucleotides AMB_RS14915 and AMB_RS14920 overlap by 4 nucleotides AMB_RS14920 and AMB_RS14925 are separated by 2 nucleotides
AMB_RS14910: AMB_RS14910 - hypothetical protein, at 3,179,276 to 3,181,957
_RS14910
AMB_RS14915: AMB_RS14915 - CAP domain-containing protein, at 3,181,973 to 3,182,872
_RS14915
AMB_RS14920: AMB_RS14920 - hypothetical protein, at 3,182,869 to 3,186,075
_RS14920
AMB_RS14925: AMB_RS14925 - penicillin-binding protein, at 3,186,078 to 3,188,435
_RS14925
Position (kb)
3182
3183
3184
3185
3186
3187 Strain fitness (log2 ratio)
-2
-1
0
1 at 3181.888 kb on + strand at 3181.889 kb on - strand at 3182.039 kb on + strand at 3182.039 kb on + strand at 3182.040 kb on - strand at 3182.040 kb on - strand at 3182.040 kb on - strand at 3182.150 kb on + strand, within AMB_RS14915 at 3182.150 kb on + strand, within AMB_RS14915 at 3182.326 kb on + strand, within AMB_RS14915 at 3182.343 kb on - strand, within AMB_RS14915 at 3182.343 kb on - strand, within AMB_RS14915 at 3182.362 kb on + strand, within AMB_RS14915 at 3182.363 kb on - strand, within AMB_RS14915 at 3182.680 kb on - strand, within AMB_RS14915 at 3182.717 kb on - strand, within AMB_RS14915 at 3183.131 kb on - strand at 3183.967 kb on - strand, within AMB_RS14920 at 3184.381 kb on + strand, within AMB_RS14920 at 3184.381 kb on + strand, within AMB_RS14920 at 3184.797 kb on + strand, within AMB_RS14920 at 3184.800 kb on + strand, within AMB_RS14920 at 3184.800 kb on + strand, within AMB_RS14920 at 3184.800 kb on + strand, within AMB_RS14920 at 3184.800 kb on + strand, within AMB_RS14920 at 3184.801 kb on - strand, within AMB_RS14920 at 3185.120 kb on + strand, within AMB_RS14920 at 3185.593 kb on + strand, within AMB_RS14920 at 3185.593 kb on + strand, within AMB_RS14920 at 3185.856 kb on + strand at 3185.922 kb on + strand at 3185.922 kb on + strand at 3185.923 kb on - strand at 3186.068 kb on + strand at 3186.068 kb on + strand at 3186.069 kb on - strand at 3186.069 kb on - strand at 3186.069 kb on - strand at 3186.069 kb on - strand at 3186.069 kb on - strand at 3186.069 kb on - strand at 3186.074 kb on - strand at 3186.079 kb on + strand at 3186.079 kb on + strand at 3186.080 kb on - strand at 3186.080 kb on - strand at 3186.080 kb on - strand at 3186.080 kb on - strand at 3186.496 kb on + strand, within AMB_RS14925 at 3186.497 kb on - strand, within AMB_RS14925 at 3186.654 kb on + strand, within AMB_RS14925 at 3186.670 kb on + strand, within AMB_RS14925 at 3186.670 kb on + strand, within AMB_RS14925 at 3186.866 kb on - strand, within AMB_RS14925
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 3,181,888 + -2.0 3,181,889 - -1.5 3,182,039 + -0.1 3,182,039 + +0.8 3,182,040 - -1.5 3,182,040 - -0.2 3,182,040 - +0.3 3,182,150 + AMB_RS14915 0.20 +1.1 3,182,150 + AMB_RS14915 0.20 +0.3 3,182,326 + AMB_RS14915 0.39 +0.1 3,182,343 - AMB_RS14915 0.41 +0.5 3,182,343 - AMB_RS14915 0.41 -0.6 3,182,362 + AMB_RS14915 0.43 +0.8 3,182,363 - AMB_RS14915 0.43 -1.1 3,182,680 - AMB_RS14915 0.79 -0.8 3,182,717 - AMB_RS14915 0.83 +0.0 3,183,131 - -0.3 3,183,967 - AMB_RS14920 0.34 -0.1 3,184,381 + AMB_RS14920 0.47 -0.1 3,184,381 + AMB_RS14920 0.47 -0.2 3,184,797 + AMB_RS14920 0.60 -0.5 3,184,800 + AMB_RS14920 0.60 +0.2 3,184,800 + AMB_RS14920 0.60 -0.1 3,184,800 + AMB_RS14920 0.60 -0.5 3,184,800 + AMB_RS14920 0.60 -1.0 3,184,801 - AMB_RS14920 0.60 -1.3 3,185,120 + AMB_RS14920 0.70 +0.0 3,185,593 + AMB_RS14920 0.85 +0.0 3,185,593 + AMB_RS14920 0.85 -1.5 3,185,856 + -1.5 3,185,922 + +0.6 3,185,922 + -0.9 3,185,923 - -1.3 3,186,068 + -2.5 3,186,068 + +1.4 3,186,069 - -1.9 3,186,069 - -1.3 3,186,069 - -0.0 3,186,069 - -0.9 3,186,069 - +0.2 3,186,069 - +0.1 3,186,074 - -2.0 3,186,079 + +0.5 3,186,079 + -0.9 3,186,080 - -0.1 3,186,080 - +0.8 3,186,080 - -0.9 3,186,080 - -0.8 3,186,496 + AMB_RS14925 0.18 -0.4 3,186,497 - AMB_RS14925 0.18 -1.1 3,186,654 + AMB_RS14925 0.24 -0.9 3,186,670 + AMB_RS14925 0.25 -0.5 3,186,670 + AMB_RS14925 0.25 -0.0 3,186,866 - AMB_RS14925 0.33 -0.5
Or see this region's nucleotide sequence