Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14525 and AMB_RS14530 overlap by 86 nucleotides AMB_RS14530 and AMB_RS14535 overlap by 4 nucleotides AMB_RS14535 and AMB_RS14540 are separated by 66 nucleotides AMB_RS14540 and AMB_RS14545 overlap by 4 nucleotides
AMB_RS14525: AMB_RS14525 - xylanase/chitin deacetylase, at 3,084,190 to 3,085,071
_RS14525
AMB_RS14530: AMB_RS14530 - acyl dehydratase, at 3,084,986 to 3,085,453
_RS14530
AMB_RS14535: AMB_RS14535 - MFS transporter, at 3,085,450 to 3,086,739
_RS14535
AMB_RS14540: AMB_RS14540 - TetR/AcrR family transcriptional regulator, at 3,086,806 to 3,087,456
_RS14540
AMB_RS14545: AMB_RS14545 - NADH:flavin oxidoreductase/NADH oxidase, at 3,087,453 to 3,088,568
_RS14545
Position (kb)
3085
3086
3087 Strain fitness (log2 ratio)
-1
0
1 at 3084.501 kb on - strand, within AMB_RS14525 at 3085.398 kb on + strand, within AMB_RS14530 at 3085.398 kb on + strand, within AMB_RS14530 at 3085.398 kb on + strand, within AMB_RS14530 at 3085.398 kb on + strand, within AMB_RS14530 at 3085.443 kb on + strand at 3085.444 kb on - strand at 3085.731 kb on - strand, within AMB_RS14535 at 3085.799 kb on - strand, within AMB_RS14535 at 3085.817 kb on + strand, within AMB_RS14535 at 3085.817 kb on + strand, within AMB_RS14535 at 3086.011 kb on - strand, within AMB_RS14535 at 3086.544 kb on - strand, within AMB_RS14535 at 3086.544 kb on - strand, within AMB_RS14535 at 3086.696 kb on + strand at 3086.697 kb on - strand at 3086.762 kb on + strand at 3086.924 kb on - strand, within AMB_RS14540 at 3087.058 kb on + strand, within AMB_RS14540 at 3087.058 kb on + strand, within AMB_RS14540 at 3087.058 kb on + strand, within AMB_RS14540 at 3087.058 kb on + strand, within AMB_RS14540 at 3087.059 kb on - strand, within AMB_RS14540 at 3087.059 kb on - strand, within AMB_RS14540 at 3087.081 kb on - strand, within AMB_RS14540 at 3087.347 kb on - strand, within AMB_RS14540 at 3087.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 3,084,501 - AMB_RS14525 0.35 +0.2 3,085,398 + AMB_RS14530 0.88 -1.1 3,085,398 + AMB_RS14530 0.88 +0.3 3,085,398 + AMB_RS14530 0.88 -0.5 3,085,398 + AMB_RS14530 0.88 +0.1 3,085,443 + -0.8 3,085,444 - -0.0 3,085,731 - AMB_RS14535 0.22 -0.9 3,085,799 - AMB_RS14535 0.27 +0.3 3,085,817 + AMB_RS14535 0.28 -0.5 3,085,817 + AMB_RS14535 0.28 -1.3 3,086,011 - AMB_RS14535 0.43 +0.3 3,086,544 - AMB_RS14535 0.85 -0.0 3,086,544 - AMB_RS14535 0.85 -0.1 3,086,696 + -1.1 3,086,697 - -1.5 3,086,762 + -0.7 3,086,924 - AMB_RS14540 0.18 -0.4 3,087,058 + AMB_RS14540 0.39 +0.4 3,087,058 + AMB_RS14540 0.39 +0.9 3,087,058 + AMB_RS14540 0.39 -0.8 3,087,058 + AMB_RS14540 0.39 -0.2 3,087,059 - AMB_RS14540 0.39 -0.3 3,087,059 - AMB_RS14540 0.39 -0.4 3,087,081 - AMB_RS14540 0.42 -0.5 3,087,347 - AMB_RS14540 0.83 +0.6 3,087,557 - -0.0
Or see this region's nucleotide sequence