Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13530 and AMB_RS13535 overlap by 4 nucleotides AMB_RS13535 and AMB_RS13540 are separated by 11 nucleotides AMB_RS13540 and AMB_RS13545 are separated by 140 nucleotides AMB_RS13545 and AMB_RS13550 overlap by 2 nucleotides
AMB_RS13530: AMB_RS13530 - periplasmic nitrate reductase subunit alpha, at 2,881,308 to 2,883,791
_RS13530
AMB_RS13535: AMB_RS13535 - hypothetical protein, at 2,883,788 to 2,884,102
_RS13535
AMB_RS13540: AMB_RS13540 - 4Fe-4S dicluster domain-containing protein, at 2,884,114 to 2,884,644
_RS13540
AMB_RS13545: AMB_RS13545 - bifunctional diguanylate cyclase/phosphodiesterase, at 2,884,785 to 2,886,431
_RS13545
AMB_RS13550: AMB_RS13550 - NAD(+) diphosphatase, at 2,886,430 to 2,887,494
_RS13550
Position (kb)
2884
2885
2886
2887 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2883.837 kb on + strand, within AMB_RS13535 at 2883.838 kb on - strand, within AMB_RS13535 at 2883.838 kb on - strand, within AMB_RS13535 at 2883.838 kb on - strand, within AMB_RS13535 at 2884.028 kb on + strand, within AMB_RS13535 at 2884.028 kb on + strand, within AMB_RS13535 at 2884.028 kb on + strand, within AMB_RS13535 at 2884.032 kb on - strand, within AMB_RS13535 at 2884.032 kb on - strand, within AMB_RS13535 at 2884.032 kb on - strand, within AMB_RS13535 at 2884.497 kb on + strand, within AMB_RS13540 at 2884.498 kb on - strand, within AMB_RS13540 at 2884.758 kb on - strand at 2884.771 kb on - strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.817 kb on + strand at 2884.818 kb on - strand at 2884.818 kb on - strand at 2884.818 kb on - strand at 2884.818 kb on - strand at 2884.852 kb on + strand at 2884.856 kb on - strand at 2884.856 kb on - strand at 2884.866 kb on + strand at 2884.866 kb on + strand at 2885.062 kb on + strand, within AMB_RS13545 at 2885.062 kb on + strand, within AMB_RS13545 at 2885.063 kb on - strand, within AMB_RS13545 at 2885.077 kb on + strand, within AMB_RS13545 at 2885.503 kb on + strand, within AMB_RS13545 at 2885.503 kb on + strand, within AMB_RS13545 at 2885.504 kb on - strand, within AMB_RS13545 at 2885.504 kb on - strand, within AMB_RS13545 at 2885.606 kb on - strand, within AMB_RS13545 at 2885.633 kb on - strand, within AMB_RS13545 at 2885.633 kb on - strand, within AMB_RS13545 at 2886.071 kb on - strand, within AMB_RS13545 at 2886.134 kb on - strand, within AMB_RS13545 at 2886.161 kb on + strand, within AMB_RS13545 at 2886.162 kb on - strand, within AMB_RS13545 at 2886.162 kb on - strand, within AMB_RS13545 at 2886.260 kb on - strand, within AMB_RS13545 at 2886.260 kb on - strand, within AMB_RS13545 at 2886.308 kb on + strand at 2886.308 kb on + strand at 2886.308 kb on + strand at 2886.487 kb on - strand at 2886.487 kb on - strand at 2886.584 kb on - strand, within AMB_RS13550 at 2886.584 kb on - strand, within AMB_RS13550 at 2886.667 kb on + strand, within AMB_RS13550 at 2886.667 kb on + strand, within AMB_RS13550 at 2886.739 kb on + strand, within AMB_RS13550 at 2886.739 kb on + strand, within AMB_RS13550 at 2886.739 kb on + strand, within AMB_RS13550 at 2886.739 kb on + strand, within AMB_RS13550 at 2886.740 kb on - strand, within AMB_RS13550 at 2886.740 kb on - strand, within AMB_RS13550 at 2886.931 kb on + strand, within AMB_RS13550 at 2886.931 kb on + strand, within AMB_RS13550 at 2887.133 kb on + strand, within AMB_RS13550 at 2887.150 kb on + strand, within AMB_RS13550 at 2887.150 kb on + strand, within AMB_RS13550 at 2887.150 kb on + strand, within AMB_RS13550 at 2887.150 kb on + strand, within AMB_RS13550 at 2887.150 kb on + strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.151 kb on - strand, within AMB_RS13550 at 2887.252 kb on + strand, within AMB_RS13550 at 2887.252 kb on + strand, within AMB_RS13550 at 2887.252 kb on + strand, within AMB_RS13550 at 2887.252 kb on + strand, within AMB_RS13550 at 2887.252 kb on + strand, within AMB_RS13550 at 2887.253 kb on - strand, within AMB_RS13550 at 2887.253 kb on - strand, within AMB_RS13550 at 2887.253 kb on - strand, within AMB_RS13550 at 2887.253 kb on - strand, within AMB_RS13550
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 2,883,837 + AMB_RS13535 0.16 -2.0 2,883,838 - AMB_RS13535 0.16 -0.3 2,883,838 - AMB_RS13535 0.16 +0.3 2,883,838 - AMB_RS13535 0.16 -1.3 2,884,028 + AMB_RS13535 0.76 -1.2 2,884,028 + AMB_RS13535 0.76 -1.3 2,884,028 + AMB_RS13535 0.76 -1.4 2,884,032 - AMB_RS13535 0.77 -0.6 2,884,032 - AMB_RS13535 0.77 -1.6 2,884,032 - AMB_RS13535 0.77 -0.1 2,884,497 + AMB_RS13540 0.72 -0.8 2,884,498 - AMB_RS13540 0.72 -1.6 2,884,758 - +0.2 2,884,771 - -0.8 2,884,817 + -1.1 2,884,817 + -0.4 2,884,817 + -2.6 2,884,817 + +0.1 2,884,817 + -0.8 2,884,817 + -2.9 2,884,817 + -0.8 2,884,818 - -0.3 2,884,818 - +0.5 2,884,818 - -1.3 2,884,818 - +0.5 2,884,852 + +0.0 2,884,856 - +0.5 2,884,856 - +0.4 2,884,866 + -0.1 2,884,866 + -1.9 2,885,062 + AMB_RS13545 0.17 -4.7 2,885,062 + AMB_RS13545 0.17 -0.3 2,885,063 - AMB_RS13545 0.17 +0.1 2,885,077 + AMB_RS13545 0.18 -1.5 2,885,503 + AMB_RS13545 0.44 -2.3 2,885,503 + AMB_RS13545 0.44 -2.3 2,885,504 - AMB_RS13545 0.44 -1.9 2,885,504 - AMB_RS13545 0.44 +0.1 2,885,606 - AMB_RS13545 0.50 -3.3 2,885,633 - AMB_RS13545 0.51 -0.6 2,885,633 - AMB_RS13545 0.51 -0.1 2,886,071 - AMB_RS13545 0.78 +0.1 2,886,134 - AMB_RS13545 0.82 +0.5 2,886,161 + AMB_RS13545 0.84 -0.6 2,886,162 - AMB_RS13545 0.84 -1.1 2,886,162 - AMB_RS13545 0.84 +0.1 2,886,260 - AMB_RS13545 0.90 +0.1 2,886,260 - AMB_RS13545 0.90 +1.9 2,886,308 + +0.0 2,886,308 + +0.5 2,886,308 + +0.1 2,886,487 - +0.5 2,886,487 - +0.5 2,886,584 - AMB_RS13550 0.14 -0.8 2,886,584 - AMB_RS13550 0.14 -0.3 2,886,667 + AMB_RS13550 0.22 +0.5 2,886,667 + AMB_RS13550 0.22 +0.4 2,886,739 + AMB_RS13550 0.29 +0.0 2,886,739 + AMB_RS13550 0.29 -0.1 2,886,739 + AMB_RS13550 0.29 +0.5 2,886,739 + AMB_RS13550 0.29 +1.1 2,886,740 - AMB_RS13550 0.29 -2.6 2,886,740 - AMB_RS13550 0.29 +0.1 2,886,931 + AMB_RS13550 0.47 -2.3 2,886,931 + AMB_RS13550 0.47 +0.7 2,887,133 + AMB_RS13550 0.66 -0.1 2,887,150 + AMB_RS13550 0.68 -0.1 2,887,150 + AMB_RS13550 0.68 +0.6 2,887,150 + AMB_RS13550 0.68 +0.4 2,887,150 + AMB_RS13550 0.68 -1.5 2,887,150 + AMB_RS13550 0.68 -1.1 2,887,151 - AMB_RS13550 0.68 -2.5 2,887,151 - AMB_RS13550 0.68 +0.9 2,887,151 - AMB_RS13550 0.68 +0.6 2,887,151 - AMB_RS13550 0.68 -3.3 2,887,151 - AMB_RS13550 0.68 -0.3 2,887,151 - AMB_RS13550 0.68 +1.1 2,887,151 - AMB_RS13550 0.68 -0.0 2,887,151 - AMB_RS13550 0.68 -0.0 2,887,151 - AMB_RS13550 0.68 -0.8 2,887,252 + AMB_RS13550 0.77 -1.5 2,887,252 + AMB_RS13550 0.77 -0.4 2,887,252 + AMB_RS13550 0.77 -0.1 2,887,252 + AMB_RS13550 0.77 -0.8 2,887,252 + AMB_RS13550 0.77 +0.8 2,887,253 - AMB_RS13550 0.77 -0.8 2,887,253 - AMB_RS13550 0.77 -0.5 2,887,253 - AMB_RS13550 0.77 +0.7 2,887,253 - AMB_RS13550 0.77 +0.5
Or see this region's nucleotide sequence