Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13510 and AMB_RS13515 are separated by 20 nucleotides AMB_RS13515 and AMB_RS13520 are separated by 36 nucleotides AMB_RS13520 and AMB_RS13525 overlap by 4 nucleotides AMB_RS13525 and AMB_RS13530 are separated by 59 nucleotides
AMB_RS13510: AMB_RS13510 - cytochrome C protein NapC, at 2,878,350 to 2,878,991
_RS13510
AMB_RS13515: AMB_RS13515 - nitrate reductase cytochrome c-type subunit, at 2,879,012 to 2,879,467
_RS13515
AMB_RS13520: AMB_RS13520 - quinol dehydrogenase ferredoxin subunit NapH, at 2,879,504 to 2,880,424
_RS13520
AMB_RS13525: AMB_RS13525 - ferredoxin-type protein NapG, at 2,880,421 to 2,881,248
_RS13525
AMB_RS13530: AMB_RS13530 - periplasmic nitrate reductase subunit alpha, at 2,881,308 to 2,883,791
_RS13530
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2878.602 kb on - strand, within AMB_RS13510 at 2878.694 kb on - strand, within AMB_RS13510 at 2878.694 kb on - strand, within AMB_RS13510 at 2878.694 kb on - strand, within AMB_RS13510 at 2878.775 kb on - strand, within AMB_RS13510 at 2879.196 kb on + strand, within AMB_RS13515 at 2879.197 kb on - strand, within AMB_RS13515 at 2879.325 kb on + strand, within AMB_RS13515 at 2879.458 kb on + strand at 2879.459 kb on - strand at 2879.459 kb on - strand at 2879.459 kb on - strand at 2879.775 kb on + strand, within AMB_RS13520 at 2879.776 kb on - strand, within AMB_RS13520 at 2879.776 kb on - strand, within AMB_RS13520 at 2879.776 kb on - strand, within AMB_RS13520 at 2879.776 kb on - strand, within AMB_RS13520 at 2879.804 kb on - strand, within AMB_RS13520 at 2879.804 kb on - strand, within AMB_RS13520 at 2880.096 kb on + strand, within AMB_RS13520 at 2880.096 kb on + strand, within AMB_RS13520 at 2880.097 kb on - strand, within AMB_RS13520 at 2880.097 kb on - strand, within AMB_RS13520 at 2880.100 kb on + strand, within AMB_RS13520 at 2880.100 kb on + strand, within AMB_RS13520 at 2880.100 kb on + strand, within AMB_RS13520 at 2880.100 kb on + strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.101 kb on - strand, within AMB_RS13520 at 2880.120 kb on + strand, within AMB_RS13520 at 2880.121 kb on - strand, within AMB_RS13520 at 2880.147 kb on - strand, within AMB_RS13520 at 2880.468 kb on + strand at 2880.469 kb on - strand at 2880.469 kb on - strand at 2880.470 kb on + strand at 2880.470 kb on + strand at 2880.471 kb on - strand at 2880.838 kb on + strand, within AMB_RS13525 at 2881.020 kb on - strand, within AMB_RS13525 at 2881.020 kb on - strand, within AMB_RS13525 at 2881.310 kb on - strand at 2881.310 kb on - strand at 2881.342 kb on + strand at 2881.343 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 2,878,602 - AMB_RS13510 0.39 -2.0 2,878,694 - AMB_RS13510 0.54 +0.5 2,878,694 - AMB_RS13510 0.54 -1.9 2,878,694 - AMB_RS13510 0.54 -1.1 2,878,775 - AMB_RS13510 0.66 -1.8 2,879,196 + AMB_RS13515 0.40 -1.3 2,879,197 - AMB_RS13515 0.41 -1.0 2,879,325 + AMB_RS13515 0.69 -1.5 2,879,458 + +0.5 2,879,459 - -0.7 2,879,459 - -1.7 2,879,459 - -0.7 2,879,775 + AMB_RS13520 0.29 -1.7 2,879,776 - AMB_RS13520 0.30 -0.3 2,879,776 - AMB_RS13520 0.30 -0.9 2,879,776 - AMB_RS13520 0.30 +0.2 2,879,776 - AMB_RS13520 0.30 -1.9 2,879,804 - AMB_RS13520 0.33 -0.1 2,879,804 - AMB_RS13520 0.33 -1.4 2,880,096 + AMB_RS13520 0.64 -0.5 2,880,096 + AMB_RS13520 0.64 -0.8 2,880,097 - AMB_RS13520 0.64 -0.8 2,880,097 - AMB_RS13520 0.64 +1.5 2,880,100 + AMB_RS13520 0.65 -0.8 2,880,100 + AMB_RS13520 0.65 +0.5 2,880,100 + AMB_RS13520 0.65 -1.5 2,880,100 + AMB_RS13520 0.65 -1.4 2,880,101 - AMB_RS13520 0.65 -1.5 2,880,101 - AMB_RS13520 0.65 -1.8 2,880,101 - AMB_RS13520 0.65 -0.5 2,880,101 - AMB_RS13520 0.65 -1.7 2,880,101 - AMB_RS13520 0.65 -3.4 2,880,101 - AMB_RS13520 0.65 -0.5 2,880,101 - AMB_RS13520 0.65 -1.1 2,880,120 + AMB_RS13520 0.67 -0.7 2,880,121 - AMB_RS13520 0.67 +1.5 2,880,147 - AMB_RS13520 0.70 -1.0 2,880,468 + -1.5 2,880,469 - -0.9 2,880,469 - -1.7 2,880,470 + -1.3 2,880,470 + -0.1 2,880,471 - -0.1 2,880,838 + AMB_RS13525 0.50 -2.3 2,881,020 - AMB_RS13525 0.72 -1.4 2,881,020 - AMB_RS13525 0.72 -0.1 2,881,310 - -0.4 2,881,310 - -0.6 2,881,342 + -1.7 2,881,343 - -0.3
Or see this region's nucleotide sequence