Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12670 and AMB_RS12675 overlap by 1 nucleotides AMB_RS12675 and AMB_RS12680 are separated by 1 nucleotides AMB_RS12680 and AMB_RS12685 are separated by 14 nucleotides AMB_RS12685 and AMB_RS12690 are separated by 10 nucleotides
AMB_RS12670: AMB_RS12670 - protein-L-isoaspartate O-methyltransferase, at 2,701,453 to 2,702,097
_RS12670
AMB_RS12675: AMB_RS12675 - 5'-nucleotidase SurE, at 2,702,097 to 2,702,879
_RS12675
AMB_RS12680: AMB_RS12680 - serine--tRNA ligase, at 2,702,881 to 2,704,149
_RS12680
AMB_RS12685: AMB_RS12685 - twin-arginine translocase subunit TatC, at 2,704,164 to 2,704,988
_RS12685
AMB_RS12690: AMB_RS12690 - twin-arginine translocase subunit TatB, at 2,704,999 to 2,705,370
_RS12690
Position (kb)
2702
2703
2704
2705 Strain fitness (log2 ratio)
-2
-1
0
1 at 2701.959 kb on - strand, within AMB_RS12670 at 2701.959 kb on - strand, within AMB_RS12670 at 2702.246 kb on - strand, within AMB_RS12675 at 2702.536 kb on + strand, within AMB_RS12675 at 2702.537 kb on - strand, within AMB_RS12675
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 2,701,959 - AMB_RS12670 0.78 -0.1 2,701,959 - AMB_RS12670 0.78 -0.4 2,702,246 - AMB_RS12675 0.19 -0.5 2,702,536 + AMB_RS12675 0.56 -1.1 2,702,537 - AMB_RS12675 0.56 -2.6
Or see this region's nucleotide sequence