Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12400 and AMB_RS12405 are separated by 91 nucleotides AMB_RS12405 and AMB_RS12410 are separated by 382 nucleotides
AMB_RS12400: AMB_RS12400 - ATP-dependent DNA helicase RecG, at 2,645,853 to 2,647,934
_RS12400
AMB_RS12405: AMB_RS12405 - UPF0016 domain-containing protein, at 2,648,026 to 2,648,607
_RS12405
AMB_RS12410: AMB_RS12410 - excinuclease ABC subunit UvrA, at 2,648,990 to 2,651,833
_RS12410
Position (kb)
2648
2649 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2647.921 kb on + strand at 2647.974 kb on + strand at 2647.974 kb on + strand at 2647.975 kb on - strand at 2647.975 kb on - strand at 2647.975 kb on - strand at 2648.100 kb on + strand, within AMB_RS12405 at 2648.100 kb on + strand, within AMB_RS12405 at 2648.608 kb on - strand at 2648.781 kb on + strand at 2648.805 kb on + strand at 2648.805 kb on + strand at 2648.806 kb on - strand at 2648.806 kb on - strand at 2648.836 kb on + strand at 2648.857 kb on + strand at 2648.857 kb on + strand at 2648.982 kb on + strand at 2648.989 kb on + strand at 2648.989 kb on + strand at 2649.041 kb on - strand at 2649.128 kb on + strand at 2649.150 kb on - strand at 2649.150 kb on - strand at 2649.170 kb on + strand at 2649.305 kb on + strand, within AMB_RS12410 at 2649.306 kb on - strand, within AMB_RS12410 at 2649.311 kb on + strand, within AMB_RS12410 at 2649.311 kb on + strand, within AMB_RS12410 at 2649.312 kb on - strand, within AMB_RS12410
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 2,647,921 + -0.2 2,647,974 + +0.0 2,647,974 + -1.7 2,647,975 - +0.1 2,647,975 - -1.1 2,647,975 - +0.1 2,648,100 + AMB_RS12405 0.13 -0.4 2,648,100 + AMB_RS12405 0.13 -2.3 2,648,608 - +0.3 2,648,781 + -0.5 2,648,805 + +0.3 2,648,805 + -2.6 2,648,806 - -0.1 2,648,806 - -0.1 2,648,836 + +0.2 2,648,857 + -1.7 2,648,857 + -0.5 2,648,982 + +0.0 2,648,989 + -0.3 2,648,989 + -0.4 2,649,041 - -0.8 2,649,128 + -4.6 2,649,150 - +0.8 2,649,150 - -1.8 2,649,170 + -0.7 2,649,305 + AMB_RS12410 0.11 -2.2 2,649,306 - AMB_RS12410 0.11 -1.7 2,649,311 + AMB_RS12410 0.11 -1.3 2,649,311 + AMB_RS12410 0.11 -0.8 2,649,312 - AMB_RS12410 0.11 -1.7
Or see this region's nucleotide sequence