Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10930 and AMB_RS10935 overlap by 1 nucleotides AMB_RS10935 and AMB_RS10940 are separated by 127 nucleotides AMB_RS10940 and AMB_RS10945 are separated by 2 nucleotides
AMB_RS10930: AMB_RS10930 - methyltransferase domain-containing protein, at 2,346,395 to 2,347,162
_RS10930
AMB_RS10935: AMB_RS10935 - hypothetical protein, at 2,347,162 to 2,347,389
_RS10935
AMB_RS10940: AMB_RS10940 - PAS domain S-box protein, at 2,347,517 to 2,350,099
_RS10940
AMB_RS10945: AMB_RS10945 - diguanylate phosphodiesterase, at 2,350,102 to 2,351,292
_RS10945
Position (kb)
2347
2348
2349
2350
2351 Strain fitness (log2 ratio)
-2
-1
0
1 at 2346.732 kb on - strand, within AMB_RS10930 at 2346.773 kb on - strand, within AMB_RS10930 at 2346.864 kb on + strand, within AMB_RS10930 at 2346.864 kb on + strand, within AMB_RS10930 at 2346.865 kb on - strand, within AMB_RS10930 at 2346.865 kb on - strand, within AMB_RS10930 at 2346.865 kb on - strand, within AMB_RS10930 at 2346.865 kb on - strand, within AMB_RS10930 at 2346.919 kb on + strand, within AMB_RS10930 at 2346.920 kb on - strand, within AMB_RS10930 at 2347.153 kb on + strand at 2347.153 kb on + strand at 2347.154 kb on - strand at 2347.384 kb on + strand at 2347.388 kb on - strand at 2347.388 kb on - strand at 2347.388 kb on - strand at 2347.409 kb on + strand at 2347.446 kb on + strand at 2347.447 kb on - strand at 2347.454 kb on - strand at 2347.563 kb on - strand at 2347.987 kb on + strand, within AMB_RS10940 at 2347.987 kb on + strand, within AMB_RS10940 at 2347.988 kb on - strand, within AMB_RS10940 at 2348.004 kb on + strand, within AMB_RS10940 at 2348.004 kb on + strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.005 kb on - strand, within AMB_RS10940 at 2348.036 kb on + strand, within AMB_RS10940 at 2348.037 kb on - strand, within AMB_RS10940 at 2348.037 kb on - strand, within AMB_RS10940 at 2348.076 kb on - strand, within AMB_RS10940 at 2348.097 kb on - strand, within AMB_RS10940 at 2348.203 kb on + strand, within AMB_RS10940 at 2348.204 kb on - strand, within AMB_RS10940 at 2348.204 kb on - strand, within AMB_RS10940 at 2348.481 kb on - strand, within AMB_RS10940 at 2349.289 kb on + strand, within AMB_RS10940 at 2349.444 kb on + strand, within AMB_RS10940 at 2349.459 kb on - strand, within AMB_RS10940 at 2349.482 kb on - strand, within AMB_RS10940 at 2349.517 kb on + strand, within AMB_RS10940 at 2349.518 kb on - strand, within AMB_RS10940 at 2349.584 kb on + strand, within AMB_RS10940 at 2349.624 kb on - strand, within AMB_RS10940 at 2349.624 kb on - strand, within AMB_RS10940 at 2349.659 kb on + strand, within AMB_RS10940 at 2349.660 kb on - strand, within AMB_RS10940 at 2349.660 kb on - strand, within AMB_RS10940 at 2349.914 kb on + strand at 2350.037 kb on + strand at 2350.158 kb on + strand at 2350.158 kb on + strand at 2350.158 kb on + strand at 2350.159 kb on - strand at 2350.159 kb on - strand at 2350.159 kb on - strand at 2350.243 kb on + strand, within AMB_RS10945 at 2350.243 kb on + strand, within AMB_RS10945 at 2350.244 kb on - strand, within AMB_RS10945 at 2350.870 kb on - strand, within AMB_RS10945 at 2350.870 kb on - strand, within AMB_RS10945 at 2351.020 kb on + strand, within AMB_RS10945
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 2,346,732 - AMB_RS10930 0.44 -0.5 2,346,773 - AMB_RS10930 0.49 -0.3 2,346,864 + AMB_RS10930 0.61 -0.3 2,346,864 + AMB_RS10930 0.61 -0.1 2,346,865 - AMB_RS10930 0.61 -0.9 2,346,865 - AMB_RS10930 0.61 +0.2 2,346,865 - AMB_RS10930 0.61 -0.1 2,346,865 - AMB_RS10930 0.61 +0.5 2,346,919 + AMB_RS10930 0.68 -0.2 2,346,920 - AMB_RS10930 0.68 -1.6 2,347,153 + -0.4 2,347,153 + -1.5 2,347,154 - -0.4 2,347,384 + -1.1 2,347,388 - +0.3 2,347,388 - -1.1 2,347,388 - -2.4 2,347,409 + +0.1 2,347,446 + -1.1 2,347,447 - -1.6 2,347,454 - -1.8 2,347,563 - +0.9 2,347,987 + AMB_RS10940 0.18 -1.3 2,347,987 + AMB_RS10940 0.18 +1.5 2,347,988 - AMB_RS10940 0.18 -0.9 2,348,004 + AMB_RS10940 0.19 -1.1 2,348,004 + AMB_RS10940 0.19 -0.9 2,348,005 - AMB_RS10940 0.19 -0.3 2,348,005 - AMB_RS10940 0.19 +0.4 2,348,005 - AMB_RS10940 0.19 +0.2 2,348,005 - AMB_RS10940 0.19 -1.9 2,348,005 - AMB_RS10940 0.19 +1.5 2,348,005 - AMB_RS10940 0.19 -0.2 2,348,005 - AMB_RS10940 0.19 -0.8 2,348,036 + AMB_RS10940 0.20 -1.0 2,348,037 - AMB_RS10940 0.20 +1.1 2,348,037 - AMB_RS10940 0.20 -2.0 2,348,076 - AMB_RS10940 0.22 -0.2 2,348,097 - AMB_RS10940 0.22 -0.2 2,348,203 + AMB_RS10940 0.27 +1.5 2,348,204 - AMB_RS10940 0.27 -0.1 2,348,204 - AMB_RS10940 0.27 +1.5 2,348,481 - AMB_RS10940 0.37 -1.1 2,349,289 + AMB_RS10940 0.69 -0.2 2,349,444 + AMB_RS10940 0.75 +0.5 2,349,459 - AMB_RS10940 0.75 -1.7 2,349,482 - AMB_RS10940 0.76 -0.4 2,349,517 + AMB_RS10940 0.77 -1.7 2,349,518 - AMB_RS10940 0.77 +0.1 2,349,584 + AMB_RS10940 0.80 -0.1 2,349,624 - AMB_RS10940 0.82 +0.1 2,349,624 - AMB_RS10940 0.82 -0.2 2,349,659 + AMB_RS10940 0.83 -0.4 2,349,660 - AMB_RS10940 0.83 -2.5 2,349,660 - AMB_RS10940 0.83 +0.3 2,349,914 + -1.9 2,350,037 + +0.4 2,350,158 + -0.5 2,350,158 + -2.1 2,350,158 + -1.7 2,350,159 - -1.7 2,350,159 - -0.9 2,350,159 - +0.2 2,350,243 + AMB_RS10945 0.12 +0.1 2,350,243 + AMB_RS10945 0.12 -0.7 2,350,244 - AMB_RS10945 0.12 -0.9 2,350,870 - AMB_RS10945 0.64 -0.0 2,350,870 - AMB_RS10945 0.64 +0.4 2,351,020 + AMB_RS10945 0.77 +0.5
Or see this region's nucleotide sequence