Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07720 and AMB_RS07725 are separated by 78 nucleotides AMB_RS07725 and AMB_RS07730 overlap by 10 nucleotides AMB_RS07730 and AMB_RS07735 overlap by 4 nucleotides AMB_RS07735 and AMB_RS07740 overlap by 17 nucleotides
AMB_RS07720: AMB_RS07720 - hypothetical protein, at 1,658,672 to 1,660,207
_RS07720
AMB_RS07725: AMB_RS07725 - universal stress protein UspA, at 1,660,286 to 1,661,152
_RS07725
AMB_RS07730: AMB_RS07730 - NUDIX hydrolase, at 1,661,143 to 1,661,859
_RS07730
AMB_RS07735: AMB_RS07735 - histidine phosphatase family protein, at 1,661,856 to 1,662,440
_RS07735
AMB_RS07740: AMB_RS07740 - demethoxyubiquinone hydroxylase family protein, at 1,662,424 to 1,662,981
_RS07740
Position (kb)
1661
1662 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1660.203 kb on + strand at 1660.204 kb on - strand at 1660.253 kb on + strand at 1660.253 kb on + strand at 1660.253 kb on + strand at 1660.254 kb on - strand at 1660.254 kb on - strand at 1660.254 kb on - strand at 1660.254 kb on - strand at 1660.254 kb on - strand at 1660.256 kb on + strand at 1660.256 kb on + strand at 1660.256 kb on + strand at 1660.257 kb on - strand at 1660.257 kb on - strand at 1660.937 kb on + strand, within AMB_RS07725 at 1660.937 kb on + strand, within AMB_RS07725 at 1660.937 kb on + strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1660.938 kb on - strand, within AMB_RS07725 at 1661.060 kb on + strand, within AMB_RS07725 at 1661.060 kb on + strand, within AMB_RS07725 at 1661.060 kb on + strand, within AMB_RS07725 at 1661.060 kb on + strand, within AMB_RS07725 at 1661.061 kb on - strand, within AMB_RS07725 at 1661.061 kb on - strand, within AMB_RS07725 at 1661.061 kb on - strand, within AMB_RS07725 at 1661.062 kb on + strand, within AMB_RS07725 at 1661.368 kb on + strand, within AMB_RS07730 at 1661.368 kb on + strand, within AMB_RS07730 at 1661.369 kb on - strand, within AMB_RS07730 at 1661.369 kb on - strand, within AMB_RS07730 at 1661.369 kb on - strand, within AMB_RS07730 at 1661.369 kb on - strand, within AMB_RS07730 at 1661.369 kb on - strand, within AMB_RS07730 at 1661.437 kb on + strand, within AMB_RS07730 at 1661.437 kb on + strand, within AMB_RS07730 at 1661.438 kb on - strand, within AMB_RS07730 at 1661.438 kb on - strand, within AMB_RS07730 at 1661.442 kb on + strand, within AMB_RS07730 at 1661.580 kb on + strand, within AMB_RS07730 at 1661.581 kb on - strand, within AMB_RS07730 at 1661.686 kb on + strand, within AMB_RS07730 at 1661.686 kb on + strand, within AMB_RS07730 at 1662.199 kb on - strand, within AMB_RS07735 at 1662.620 kb on + strand, within AMB_RS07740 at 1662.780 kb on + strand, within AMB_RS07740
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,660,203 + -0.5 1,660,204 - -2.0 1,660,253 + -2.8 1,660,253 + -1.3 1,660,253 + -1.7 1,660,254 - +0.1 1,660,254 - +0.5 1,660,254 - +1.5 1,660,254 - -1.1 1,660,254 - +0.5 1,660,256 + -0.0 1,660,256 + -0.1 1,660,256 + +0.1 1,660,257 - +0.2 1,660,257 - -0.3 1,660,937 + AMB_RS07725 0.75 +0.6 1,660,937 + AMB_RS07725 0.75 -2.0 1,660,937 + AMB_RS07725 0.75 +0.1 1,660,938 - AMB_RS07725 0.75 -0.1 1,660,938 - AMB_RS07725 0.75 +0.9 1,660,938 - AMB_RS07725 0.75 -0.2 1,660,938 - AMB_RS07725 0.75 -0.1 1,660,938 - AMB_RS07725 0.75 +1.1 1,660,938 - AMB_RS07725 0.75 +1.1 1,660,938 - AMB_RS07725 0.75 -0.1 1,661,060 + AMB_RS07725 0.89 +0.3 1,661,060 + AMB_RS07725 0.89 +0.9 1,661,060 + AMB_RS07725 0.89 -2.2 1,661,060 + AMB_RS07725 0.89 -0.5 1,661,061 - AMB_RS07725 0.89 -0.1 1,661,061 - AMB_RS07725 0.89 -0.2 1,661,061 - AMB_RS07725 0.89 +0.0 1,661,062 + AMB_RS07725 0.90 -0.4 1,661,368 + AMB_RS07730 0.31 +0.1 1,661,368 + AMB_RS07730 0.31 -0.8 1,661,369 - AMB_RS07730 0.32 -0.0 1,661,369 - AMB_RS07730 0.32 -2.3 1,661,369 - AMB_RS07730 0.32 -1.5 1,661,369 - AMB_RS07730 0.32 +0.4 1,661,369 - AMB_RS07730 0.32 -0.1 1,661,437 + AMB_RS07730 0.41 -0.3 1,661,437 + AMB_RS07730 0.41 -0.7 1,661,438 - AMB_RS07730 0.41 -0.1 1,661,438 - AMB_RS07730 0.41 -1.1 1,661,442 + AMB_RS07730 0.42 -1.3 1,661,580 + AMB_RS07730 0.61 -0.5 1,661,581 - AMB_RS07730 0.61 -1.5 1,661,686 + AMB_RS07730 0.76 -2.2 1,661,686 + AMB_RS07730 0.76 -0.1 1,662,199 - AMB_RS07735 0.59 -0.4 1,662,620 + AMB_RS07740 0.35 -2.0 1,662,780 + AMB_RS07740 0.64 +1.5
Or see this region's nucleotide sequence