Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07525 and AMB_RS07530 are separated by 292 nucleotides AMB_RS07530 and AMB_RS07535 are separated by 71 nucleotides AMB_RS07535 and AMB_RS07540 are separated by 188 nucleotides
AMB_RS07525: AMB_RS07525 - sigma-70 family RNA polymerase sigma factor, at 1,629,905 to 1,630,969
_RS07525
AMB_RS07530: AMB_RS07530 - site-specific integrase, at 1,631,262 to 1,632,332
_RS07530
AMB_RS07535: AMB_RS07535 - hypothetical protein, at 1,632,404 to 1,632,673
_RS07535
AMB_RS07540: AMB_RS07540 - reverse transcriptase, at 1,632,862 to 1,634,274
_RS07540
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1630.386 kb on + strand, within AMB_RS07525 at 1630.386 kb on + strand, within AMB_RS07525 at 1630.386 kb on + strand, within AMB_RS07525 at 1630.387 kb on - strand, within AMB_RS07525 at 1630.387 kb on - strand, within AMB_RS07525 at 1630.387 kb on - strand, within AMB_RS07525 at 1630.387 kb on - strand, within AMB_RS07525 at 1630.497 kb on + strand, within AMB_RS07525 at 1630.497 kb on + strand, within AMB_RS07525 at 1630.497 kb on + strand, within AMB_RS07525 at 1630.497 kb on + strand, within AMB_RS07525 at 1630.497 kb on + strand, within AMB_RS07525 at 1630.498 kb on - strand, within AMB_RS07525 at 1630.524 kb on + strand, within AMB_RS07525 at 1630.524 kb on + strand, within AMB_RS07525 at 1630.524 kb on + strand, within AMB_RS07525 at 1630.524 kb on + strand, within AMB_RS07525 at 1630.524 kb on + strand, within AMB_RS07525 at 1630.582 kb on + strand, within AMB_RS07525 at 1630.583 kb on - strand, within AMB_RS07525 at 1631.119 kb on + strand at 1631.149 kb on - strand at 1631.149 kb on - strand at 1631.259 kb on - strand at 1631.263 kb on + strand at 1631.264 kb on - strand at 1631.264 kb on - strand at 1631.266 kb on - strand at 1631.266 kb on - strand at 1631.318 kb on - strand at 1631.357 kb on - strand at 1631.357 kb on - strand at 1631.418 kb on + strand, within AMB_RS07530 at 1631.418 kb on + strand, within AMB_RS07530 at 1631.419 kb on - strand, within AMB_RS07530 at 1631.419 kb on - strand, within AMB_RS07530 at 1631.419 kb on - strand, within AMB_RS07530 at 1631.506 kb on + strand, within AMB_RS07530 at 1631.519 kb on - strand, within AMB_RS07530 at 1631.612 kb on + strand, within AMB_RS07530 at 1631.874 kb on + strand, within AMB_RS07530 at 1631.875 kb on - strand, within AMB_RS07530 at 1631.920 kb on + strand, within AMB_RS07530 at 1631.920 kb on + strand, within AMB_RS07530 at 1631.921 kb on - strand, within AMB_RS07530 at 1631.921 kb on - strand, within AMB_RS07530 at 1631.921 kb on - strand, within AMB_RS07530 at 1632.004 kb on + strand, within AMB_RS07530 at 1632.005 kb on - strand, within AMB_RS07530 at 1632.005 kb on - strand, within AMB_RS07530 at 1632.037 kb on + strand, within AMB_RS07530 at 1632.037 kb on + strand, within AMB_RS07530 at 1632.037 kb on + strand, within AMB_RS07530 at 1632.038 kb on - strand, within AMB_RS07530 at 1632.038 kb on - strand, within AMB_RS07530 at 1632.038 kb on - strand, within AMB_RS07530 at 1632.038 kb on - strand, within AMB_RS07530 at 1632.057 kb on + strand, within AMB_RS07530 at 1632.124 kb on + strand, within AMB_RS07530 at 1632.124 kb on + strand, within AMB_RS07530 at 1632.124 kb on + strand, within AMB_RS07530 at 1632.312 kb on + strand at 1632.313 kb on - strand at 1632.313 kb on - strand at 1632.313 kb on - strand at 1632.393 kb on + strand at 1632.393 kb on + strand at 1632.405 kb on + strand at 1632.405 kb on + strand at 1632.406 kb on - strand at 1632.539 kb on + strand, within AMB_RS07535 at 1632.572 kb on + strand, within AMB_RS07535 at 1632.679 kb on + strand at 1632.701 kb on + strand at 1632.701 kb on + strand at 1632.701 kb on + strand at 1632.760 kb on + strand at 1633.004 kb on + strand, within AMB_RS07540 at 1633.004 kb on + strand, within AMB_RS07540 at 1633.274 kb on + strand, within AMB_RS07540 at 1633.274 kb on + strand, within AMB_RS07540 at 1633.274 kb on + strand, within AMB_RS07540 at 1633.275 kb on - strand, within AMB_RS07540 at 1633.325 kb on + strand, within AMB_RS07540 at 1633.325 kb on + strand, within AMB_RS07540
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,630,386 + AMB_RS07525 0.45 -0.8 1,630,386 + AMB_RS07525 0.45 +1.5 1,630,386 + AMB_RS07525 0.45 +0.5 1,630,387 - AMB_RS07525 0.45 -0.3 1,630,387 - AMB_RS07525 0.45 -1.3 1,630,387 - AMB_RS07525 0.45 +0.1 1,630,387 - AMB_RS07525 0.45 -0.8 1,630,497 + AMB_RS07525 0.56 -3.1 1,630,497 + AMB_RS07525 0.56 -0.0 1,630,497 + AMB_RS07525 0.56 -0.7 1,630,497 + AMB_RS07525 0.56 +0.1 1,630,497 + AMB_RS07525 0.56 -0.5 1,630,498 - AMB_RS07525 0.56 +0.6 1,630,524 + AMB_RS07525 0.58 -2.0 1,630,524 + AMB_RS07525 0.58 -2.5 1,630,524 + AMB_RS07525 0.58 -0.5 1,630,524 + AMB_RS07525 0.58 -1.3 1,630,524 + AMB_RS07525 0.58 -1.1 1,630,582 + AMB_RS07525 0.64 -0.2 1,630,583 - AMB_RS07525 0.64 -0.1 1,631,119 + +0.1 1,631,149 - -0.4 1,631,149 - -1.7 1,631,259 - +0.2 1,631,263 + -1.3 1,631,264 - -0.2 1,631,264 - -1.2 1,631,266 - -0.5 1,631,266 - +1.5 1,631,318 - -0.5 1,631,357 - +0.3 1,631,357 - -0.2 1,631,418 + AMB_RS07530 0.15 +1.1 1,631,418 + AMB_RS07530 0.15 -0.1 1,631,419 - AMB_RS07530 0.15 +0.0 1,631,419 - AMB_RS07530 0.15 -1.8 1,631,419 - AMB_RS07530 0.15 +1.5 1,631,506 + AMB_RS07530 0.23 +0.3 1,631,519 - AMB_RS07530 0.24 -0.8 1,631,612 + AMB_RS07530 0.33 +0.4 1,631,874 + AMB_RS07530 0.57 +0.3 1,631,875 - AMB_RS07530 0.57 +0.0 1,631,920 + AMB_RS07530 0.61 -2.3 1,631,920 + AMB_RS07530 0.61 +0.2 1,631,921 - AMB_RS07530 0.62 +1.5 1,631,921 - AMB_RS07530 0.62 -0.5 1,631,921 - AMB_RS07530 0.62 +1.5 1,632,004 + AMB_RS07530 0.69 +0.5 1,632,005 - AMB_RS07530 0.69 -0.7 1,632,005 - AMB_RS07530 0.69 -0.8 1,632,037 + AMB_RS07530 0.72 -0.1 1,632,037 + AMB_RS07530 0.72 -2.2 1,632,037 + AMB_RS07530 0.72 -2.0 1,632,038 - AMB_RS07530 0.72 -0.5 1,632,038 - AMB_RS07530 0.72 +1.5 1,632,038 - AMB_RS07530 0.72 -1.7 1,632,038 - AMB_RS07530 0.72 -0.1 1,632,057 + AMB_RS07530 0.74 -0.1 1,632,124 + AMB_RS07530 0.80 +0.7 1,632,124 + AMB_RS07530 0.80 -2.6 1,632,124 + AMB_RS07530 0.80 +0.1 1,632,312 + +0.5 1,632,313 - -0.5 1,632,313 - -0.1 1,632,313 - -1.1 1,632,393 + -2.7 1,632,393 + -0.8 1,632,405 + -0.8 1,632,405 + +0.1 1,632,406 - +0.2 1,632,539 + AMB_RS07535 0.50 -0.5 1,632,572 + AMB_RS07535 0.62 +0.3 1,632,679 + -0.5 1,632,701 + +0.2 1,632,701 + -0.2 1,632,701 + -0.5 1,632,760 + -0.3 1,633,004 + AMB_RS07540 0.10 +0.1 1,633,004 + AMB_RS07540 0.10 +0.5 1,633,274 + AMB_RS07540 0.29 -0.1 1,633,274 + AMB_RS07540 0.29 +0.2 1,633,274 + AMB_RS07540 0.29 -1.7 1,633,275 - AMB_RS07540 0.29 -2.1 1,633,325 + AMB_RS07540 0.33 -1.1 1,633,325 + AMB_RS07540 0.33 -0.5
Or see this region's nucleotide sequence