Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06995 and AMB_RS07000 are separated by 203 nucleotides AMB_RS07000 and AMB_RS07005 are separated by 320 nucleotides AMB_RS07005 and AMB_RS07010 are separated by 54 nucleotides
AMB_RS06995: AMB_RS06995 - fructose-bisphosphatase class II, at 1,499,532 to 1,500,488
_RS06995
AMB_RS07000: AMB_RS07000 - acetyl-CoA hydrolase, at 1,500,692 to 1,502,200
_RS07000
AMB_RS07005: AMB_RS07005 - AsnC family transcriptional regulator, at 1,502,521 to 1,502,760
_RS07005
AMB_RS07010: AMB_RS07010 - ATP-dependent helicase, at 1,502,815 to 1,504,506
_RS07010
Position (kb)
1500
1501
1502
1503 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1499.993 kb on - strand, within AMB_RS06995 at 1499.993 kb on - strand, within AMB_RS06995 at 1499.993 kb on - strand, within AMB_RS06995 at 1500.116 kb on - strand, within AMB_RS06995 at 1500.278 kb on - strand, within AMB_RS06995 at 1500.278 kb on - strand, within AMB_RS06995 at 1500.278 kb on - strand, within AMB_RS06995 at 1500.416 kb on - strand at 1500.458 kb on - strand at 1500.458 kb on - strand at 1500.814 kb on - strand at 1501.113 kb on + strand, within AMB_RS07000 at 1501.113 kb on + strand, within AMB_RS07000 at 1501.113 kb on + strand, within AMB_RS07000 at 1501.114 kb on - strand, within AMB_RS07000 at 1501.114 kb on - strand, within AMB_RS07000 at 1501.218 kb on + strand, within AMB_RS07000 at 1501.218 kb on + strand, within AMB_RS07000 at 1501.344 kb on + strand, within AMB_RS07000 at 1501.344 kb on + strand, within AMB_RS07000 at 1501.344 kb on + strand, within AMB_RS07000 at 1501.594 kb on - strand, within AMB_RS07000 at 1501.664 kb on - strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.665 kb on + strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1501.666 kb on - strand, within AMB_RS07000 at 1502.196 kb on + strand at 1502.196 kb on + strand at 1502.196 kb on + strand at 1502.196 kb on + strand at 1502.197 kb on - strand at 1502.351 kb on - strand at 1502.361 kb on + strand at 1502.362 kb on - strand at 1502.384 kb on + strand at 1502.384 kb on + strand at 1502.385 kb on - strand at 1502.517 kb on + strand at 1502.517 kb on + strand at 1502.517 kb on + strand at 1502.517 kb on + strand at 1502.518 kb on - strand at 1502.627 kb on + strand, within AMB_RS07005 at 1502.627 kb on + strand, within AMB_RS07005 at 1502.627 kb on + strand, within AMB_RS07005 at 1502.627 kb on + strand, within AMB_RS07005 at 1502.627 kb on + strand, within AMB_RS07005 at 1502.628 kb on - strand, within AMB_RS07005 at 1502.628 kb on - strand, within AMB_RS07005 at 1502.628 kb on - strand, within AMB_RS07005 at 1502.628 kb on - strand, within AMB_RS07005 at 1502.648 kb on + strand, within AMB_RS07005 at 1502.714 kb on + strand, within AMB_RS07005 at 1502.714 kb on + strand, within AMB_RS07005 at 1502.714 kb on + strand, within AMB_RS07005 at 1502.714 kb on + strand, within AMB_RS07005 at 1502.808 kb on + strand at 1502.809 kb on - strand at 1502.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,499,993 - AMB_RS06995 0.48 -0.0 1,499,993 - AMB_RS06995 0.48 -1.3 1,499,993 - AMB_RS06995 0.48 -2.3 1,500,116 - AMB_RS06995 0.61 -0.8 1,500,278 - AMB_RS06995 0.78 +0.0 1,500,278 - AMB_RS06995 0.78 -0.7 1,500,278 - AMB_RS06995 0.78 -0.1 1,500,416 - -0.0 1,500,458 - +1.5 1,500,458 - -2.7 1,500,814 - -1.5 1,501,113 + AMB_RS07000 0.28 -3.1 1,501,113 + AMB_RS07000 0.28 +0.3 1,501,113 + AMB_RS07000 0.28 -0.0 1,501,114 - AMB_RS07000 0.28 -1.1 1,501,114 - AMB_RS07000 0.28 -0.3 1,501,218 + AMB_RS07000 0.35 -0.1 1,501,218 + AMB_RS07000 0.35 -0.3 1,501,344 + AMB_RS07000 0.43 -3.4 1,501,344 + AMB_RS07000 0.43 -0.5 1,501,344 + AMB_RS07000 0.43 +0.3 1,501,594 - AMB_RS07000 0.60 +0.1 1,501,664 - AMB_RS07000 0.64 -0.1 1,501,665 + AMB_RS07000 0.64 -0.2 1,501,665 + AMB_RS07000 0.64 +0.5 1,501,665 + AMB_RS07000 0.64 -0.3 1,501,665 + AMB_RS07000 0.64 +0.2 1,501,665 + AMB_RS07000 0.64 -0.6 1,501,665 + AMB_RS07000 0.64 +0.5 1,501,666 - AMB_RS07000 0.65 -0.8 1,501,666 - AMB_RS07000 0.65 +1.5 1,501,666 - AMB_RS07000 0.65 -0.5 1,501,666 - AMB_RS07000 0.65 -0.5 1,501,666 - AMB_RS07000 0.65 +0.1 1,501,666 - AMB_RS07000 0.65 -1.1 1,501,666 - AMB_RS07000 0.65 -1.5 1,501,666 - AMB_RS07000 0.65 -0.5 1,501,666 - AMB_RS07000 0.65 -0.2 1,501,666 - AMB_RS07000 0.65 -1.3 1,501,666 - AMB_RS07000 0.65 +0.3 1,501,666 - AMB_RS07000 0.65 -0.2 1,501,666 - AMB_RS07000 0.65 -0.8 1,501,666 - AMB_RS07000 0.65 -0.2 1,502,196 + +0.2 1,502,196 + +0.4 1,502,196 + +0.4 1,502,196 + -1.4 1,502,197 - -0.2 1,502,351 - +0.2 1,502,361 + -3.1 1,502,362 - +0.5 1,502,384 + +0.9 1,502,384 + -0.3 1,502,385 - -1.1 1,502,517 + -0.5 1,502,517 + -0.3 1,502,517 + +0.5 1,502,517 + -0.1 1,502,518 - -0.1 1,502,627 + AMB_RS07005 0.44 -0.1 1,502,627 + AMB_RS07005 0.44 -1.1 1,502,627 + AMB_RS07005 0.44 -0.8 1,502,627 + AMB_RS07005 0.44 -0.1 1,502,627 + AMB_RS07005 0.44 +0.3 1,502,628 - AMB_RS07005 0.45 -0.5 1,502,628 - AMB_RS07005 0.45 +0.0 1,502,628 - AMB_RS07005 0.45 -0.8 1,502,628 - AMB_RS07005 0.45 -0.8 1,502,648 + AMB_RS07005 0.53 +0.1 1,502,714 + AMB_RS07005 0.80 -0.0 1,502,714 + AMB_RS07005 0.80 -3.0 1,502,714 + AMB_RS07005 0.80 -3.4 1,502,714 + AMB_RS07005 0.80 -0.2 1,502,808 + -2.0 1,502,809 - +0.3 1,502,809 - +0.0
Or see this region's nucleotide sequence