Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06730 and AMB_RS06735 are separated by 11 nucleotides AMB_RS06735 and AMB_RS06740 are separated by 102 nucleotides AMB_RS06740 and AMB_RS06745 are separated by 103 nucleotides
AMB_RS06730: AMB_RS06730 - nitrate ABC transporter ATP-binding protein, at 1,442,665 to 1,444,023
_RS06730
AMB_RS06735: AMB_RS06735 - sulfonate ABC transporter permease, at 1,444,035 to 1,445,774
_RS06735
AMB_RS06740: AMB_RS06740 - dihydrofolate reductase, at 1,445,877 to 1,446,368
_RS06740
AMB_RS06745: AMB_RS06745 - thymidylate synthase, at 1,446,472 to 1,447,266
_RS06745
Position (kb)
1444
1445
1446 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1443.200 kb on + strand, within AMB_RS06730 at 1443.370 kb on + strand, within AMB_RS06730 at 1443.408 kb on + strand, within AMB_RS06730 at 1443.620 kb on + strand, within AMB_RS06730 at 1443.804 kb on - strand, within AMB_RS06730 at 1444.069 kb on + strand at 1444.069 kb on + strand at 1444.069 kb on + strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.070 kb on - strand at 1444.177 kb on + strand at 1444.177 kb on + strand at 1444.178 kb on - strand at 1444.178 kb on - strand at 1444.178 kb on - strand at 1444.414 kb on + strand, within AMB_RS06735 at 1444.414 kb on + strand, within AMB_RS06735 at 1444.414 kb on + strand, within AMB_RS06735 at 1444.414 kb on + strand, within AMB_RS06735 at 1444.414 kb on + strand, within AMB_RS06735 at 1444.415 kb on - strand, within AMB_RS06735 at 1444.415 kb on - strand, within AMB_RS06735 at 1444.584 kb on + strand, within AMB_RS06735 at 1444.585 kb on - strand, within AMB_RS06735 at 1444.674 kb on + strand, within AMB_RS06735 at 1444.674 kb on + strand, within AMB_RS06735 at 1444.675 kb on - strand, within AMB_RS06735 at 1445.014 kb on + strand, within AMB_RS06735 at 1445.015 kb on - strand, within AMB_RS06735 at 1445.015 kb on - strand, within AMB_RS06735 at 1445.015 kb on - strand, within AMB_RS06735 at 1445.015 kb on - strand, within AMB_RS06735 at 1445.015 kb on - strand, within AMB_RS06735 at 1445.095 kb on + strand, within AMB_RS06735 at 1445.095 kb on + strand, within AMB_RS06735 at 1445.096 kb on - strand, within AMB_RS06735 at 1445.314 kb on + strand, within AMB_RS06735 at 1445.315 kb on - strand, within AMB_RS06735 at 1445.439 kb on + strand, within AMB_RS06735 at 1445.439 kb on + strand, within AMB_RS06735 at 1445.521 kb on + strand, within AMB_RS06735 at 1445.521 kb on + strand, within AMB_RS06735 at 1445.522 kb on - strand, within AMB_RS06735 at 1445.522 kb on - strand, within AMB_RS06735 at 1445.522 kb on - strand, within AMB_RS06735 at 1445.587 kb on + strand, within AMB_RS06735 at 1445.588 kb on - strand, within AMB_RS06735 at 1445.590 kb on + strand, within AMB_RS06735 at 1445.591 kb on - strand, within AMB_RS06735 at 1445.668 kb on + strand at 1445.703 kb on + strand at 1445.704 kb on - strand at 1445.811 kb on + strand at 1445.812 kb on - strand at 1445.812 kb on - strand at 1445.850 kb on + strand at 1445.851 kb on - strand at 1445.851 kb on - strand at 1446.400 kb on - strand at 1446.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,443,200 + AMB_RS06730 0.39 -2.2 1,443,370 + AMB_RS06730 0.52 -0.5 1,443,408 + AMB_RS06730 0.55 -0.3 1,443,620 + AMB_RS06730 0.70 -0.1 1,443,804 - AMB_RS06730 0.84 +0.4 1,444,069 + +0.1 1,444,069 + -0.7 1,444,069 + -1.1 1,444,070 - -1.0 1,444,070 - -3.6 1,444,070 - -0.5 1,444,070 - -0.3 1,444,070 - -2.0 1,444,070 - -0.8 1,444,070 - -0.4 1,444,070 - -2.0 1,444,177 + -2.5 1,444,177 + +0.5 1,444,178 - -0.5 1,444,178 - +0.5 1,444,178 - +1.5 1,444,414 + AMB_RS06735 0.22 -2.6 1,444,414 + AMB_RS06735 0.22 -1.7 1,444,414 + AMB_RS06735 0.22 +0.4 1,444,414 + AMB_RS06735 0.22 +1.1 1,444,414 + AMB_RS06735 0.22 -1.7 1,444,415 - AMB_RS06735 0.22 -1.2 1,444,415 - AMB_RS06735 0.22 -0.1 1,444,584 + AMB_RS06735 0.32 +1.5 1,444,585 - AMB_RS06735 0.32 -1.8 1,444,674 + AMB_RS06735 0.37 -0.3 1,444,674 + AMB_RS06735 0.37 -1.0 1,444,675 - AMB_RS06735 0.37 -0.3 1,445,014 + AMB_RS06735 0.56 -0.2 1,445,015 - AMB_RS06735 0.56 +0.1 1,445,015 - AMB_RS06735 0.56 -0.1 1,445,015 - AMB_RS06735 0.56 -1.1 1,445,015 - AMB_RS06735 0.56 +0.5 1,445,015 - AMB_RS06735 0.56 -0.0 1,445,095 + AMB_RS06735 0.61 -2.9 1,445,095 + AMB_RS06735 0.61 -0.8 1,445,096 - AMB_RS06735 0.61 -0.8 1,445,314 + AMB_RS06735 0.74 -1.1 1,445,315 - AMB_RS06735 0.74 -1.1 1,445,439 + AMB_RS06735 0.81 +0.6 1,445,439 + AMB_RS06735 0.81 -0.5 1,445,521 + AMB_RS06735 0.85 -0.1 1,445,521 + AMB_RS06735 0.85 -1.5 1,445,522 - AMB_RS06735 0.85 -0.1 1,445,522 - AMB_RS06735 0.85 -1.3 1,445,522 - AMB_RS06735 0.85 -0.3 1,445,587 + AMB_RS06735 0.89 -0.5 1,445,588 - AMB_RS06735 0.89 -1.1 1,445,590 + AMB_RS06735 0.89 -0.4 1,445,591 - AMB_RS06735 0.89 -0.3 1,445,668 + -0.5 1,445,703 + -0.4 1,445,704 - +0.1 1,445,811 + -0.5 1,445,812 - -1.5 1,445,812 - -2.1 1,445,850 + -0.1 1,445,851 - -1.1 1,445,851 - -2.5 1,446,400 - -0.8 1,446,425 - -0.7
Or see this region's nucleotide sequence