Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23255 and AMB_RS06230 are separated by 85 nucleotides AMB_RS06230 and AMB_RS06235 are separated by 62 nucleotides AMB_RS06235 and AMB_RS23260 overlap by 4 nucleotides AMB_RS23260 and AMB_RS06245 are separated by 54 nucleotides
AMB_RS23255: AMB_RS23255 - adenylate/guanylate cyclase domain-containing protein, at 1,301,287 to 1,303,428
_RS23255
AMB_RS06230: AMB_RS06230 - 5,6-dimethylbenzimidazole synthase, at 1,303,514 to 1,304,260
_RS06230
AMB_RS06235: AMB_RS06235 - gamma-glutamylcyclotransferase, at 1,304,323 to 1,304,883
_RS06235
AMB_RS23260: AMB_RS23260 - hypothetical protein, at 1,304,880 to 1,305,791
_RS23260
AMB_RS06245: AMB_RS06245 - hypothetical protein, at 1,305,846 to 1,306,856
_RS06245
Position (kb)
1304
1305 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1303.386 kb on + strand at 1303.468 kb on + strand at 1303.468 kb on + strand at 1303.474 kb on - strand at 1303.476 kb on + strand at 1303.512 kb on + strand at 1303.512 kb on + strand at 1303.512 kb on + strand at 1303.513 kb on - strand at 1303.513 kb on - strand at 1303.513 kb on - strand at 1303.698 kb on + strand, within AMB_RS06230 at 1303.698 kb on + strand, within AMB_RS06230 at 1303.699 kb on - strand, within AMB_RS06230 at 1303.699 kb on - strand, within AMB_RS06230 at 1303.699 kb on - strand, within AMB_RS06230 at 1303.836 kb on + strand, within AMB_RS06230 at 1303.837 kb on - strand, within AMB_RS06230 at 1303.956 kb on + strand, within AMB_RS06230 at 1304.193 kb on + strand at 1304.306 kb on + strand at 1304.443 kb on + strand, within AMB_RS06235 at 1304.444 kb on - strand, within AMB_RS06235 at 1304.593 kb on + strand, within AMB_RS06235 at 1304.599 kb on + strand, within AMB_RS06235 at 1304.599 kb on + strand, within AMB_RS06235 at 1304.599 kb on + strand, within AMB_RS06235 at 1304.599 kb on + strand, within AMB_RS06235 at 1304.599 kb on + strand, within AMB_RS06235 at 1304.600 kb on - strand, within AMB_RS06235 at 1304.653 kb on + strand, within AMB_RS06235 at 1304.654 kb on - strand, within AMB_RS06235 at 1304.654 kb on - strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.655 kb on + strand, within AMB_RS06235 at 1304.656 kb on - strand, within AMB_RS06235 at 1304.686 kb on + strand, within AMB_RS06235 at 1304.686 kb on + strand, within AMB_RS06235 at 1304.686 kb on + strand, within AMB_RS06235 at 1304.686 kb on + strand, within AMB_RS06235 at 1304.764 kb on + strand, within AMB_RS06235 at 1304.764 kb on + strand, within AMB_RS06235 at 1304.765 kb on - strand, within AMB_RS06235 at 1304.765 kb on - strand, within AMB_RS06235 at 1304.881 kb on + strand at 1304.882 kb on - strand at 1305.025 kb on + strand, within AMB_RS23260 at 1305.025 kb on + strand, within AMB_RS23260 at 1305.026 kb on - strand, within AMB_RS23260 at 1305.067 kb on + strand, within AMB_RS23260 at 1305.067 kb on + strand, within AMB_RS23260 at 1305.095 kb on - strand, within AMB_RS23260 at 1305.148 kb on + strand, within AMB_RS23260 at 1305.148 kb on + strand, within AMB_RS23260 at 1305.148 kb on + strand, within AMB_RS23260 at 1305.148 kb on + strand, within AMB_RS23260 at 1305.148 kb on + strand, within AMB_RS23260 at 1305.149 kb on - strand, within AMB_RS23260 at 1305.328 kb on + strand, within AMB_RS23260 at 1305.328 kb on + strand, within AMB_RS23260 at 1305.441 kb on + strand, within AMB_RS23260 at 1305.441 kb on + strand, within AMB_RS23260 at 1305.458 kb on - strand, within AMB_RS23260 at 1305.496 kb on + strand, within AMB_RS23260 at 1305.550 kb on - strand, within AMB_RS23260 at 1305.625 kb on + strand, within AMB_RS23260 at 1305.625 kb on + strand, within AMB_RS23260 at 1305.625 kb on + strand, within AMB_RS23260 at 1305.626 kb on - strand, within AMB_RS23260 at 1305.640 kb on + strand, within AMB_RS23260 at 1305.640 kb on + strand, within AMB_RS23260 at 1305.640 kb on + strand, within AMB_RS23260 at 1305.640 kb on + strand, within AMB_RS23260 at 1305.641 kb on - strand, within AMB_RS23260 at 1305.641 kb on - strand, within AMB_RS23260 at 1305.727 kb on + strand at 1305.727 kb on + strand at 1305.742 kb on + strand at 1305.742 kb on + strand at 1305.742 kb on + strand at 1305.743 kb on - strand at 1305.743 kb on - strand at 1305.752 kb on + strand at 1305.752 kb on + strand at 1305.753 kb on - strand at 1305.753 kb on - strand at 1305.806 kb on - strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.807 kb on + strand at 1305.808 kb on - strand at 1305.808 kb on - strand at 1305.808 kb on - strand at 1305.808 kb on - strand at 1305.808 kb on - strand at 1305.808 kb on - strand at 1305.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,303,386 + -2.2 1,303,468 + +1.0 1,303,468 + +0.4 1,303,474 - -2.1 1,303,476 + +0.1 1,303,512 + +0.2 1,303,512 + -0.1 1,303,512 + -1.5 1,303,513 - -1.1 1,303,513 - -1.3 1,303,513 - -1.7 1,303,698 + AMB_RS06230 0.25 -0.2 1,303,698 + AMB_RS06230 0.25 -0.3 1,303,699 - AMB_RS06230 0.25 +0.5 1,303,699 - AMB_RS06230 0.25 -1.1 1,303,699 - AMB_RS06230 0.25 -0.3 1,303,836 + AMB_RS06230 0.43 +0.2 1,303,837 - AMB_RS06230 0.43 +0.2 1,303,956 + AMB_RS06230 0.59 -2.4 1,304,193 + -1.8 1,304,306 + -1.1 1,304,443 + AMB_RS06235 0.21 -0.6 1,304,444 - AMB_RS06235 0.22 -0.1 1,304,593 + AMB_RS06235 0.48 +0.4 1,304,599 + AMB_RS06235 0.49 +1.5 1,304,599 + AMB_RS06235 0.49 -2.2 1,304,599 + AMB_RS06235 0.49 -0.2 1,304,599 + AMB_RS06235 0.49 -1.1 1,304,599 + AMB_RS06235 0.49 -1.1 1,304,600 - AMB_RS06235 0.49 +0.2 1,304,653 + AMB_RS06235 0.59 -1.1 1,304,654 - AMB_RS06235 0.59 -0.1 1,304,654 - AMB_RS06235 0.59 -0.0 1,304,655 + AMB_RS06235 0.59 +0.6 1,304,655 + AMB_RS06235 0.59 -0.5 1,304,655 + AMB_RS06235 0.59 -0.0 1,304,655 + AMB_RS06235 0.59 +0.0 1,304,655 + AMB_RS06235 0.59 +0.5 1,304,655 + AMB_RS06235 0.59 +0.1 1,304,656 - AMB_RS06235 0.59 -2.7 1,304,686 + AMB_RS06235 0.65 +0.4 1,304,686 + AMB_RS06235 0.65 +0.4 1,304,686 + AMB_RS06235 0.65 -0.3 1,304,686 + AMB_RS06235 0.65 +0.2 1,304,764 + AMB_RS06235 0.79 +0.2 1,304,764 + AMB_RS06235 0.79 -3.8 1,304,765 - AMB_RS06235 0.79 -1.7 1,304,765 - AMB_RS06235 0.79 +0.2 1,304,881 + +0.1 1,304,882 - +1.1 1,305,025 + AMB_RS23260 0.16 -1.1 1,305,025 + AMB_RS23260 0.16 -1.1 1,305,026 - AMB_RS23260 0.16 -3.1 1,305,067 + AMB_RS23260 0.21 -0.8 1,305,067 + AMB_RS23260 0.21 -1.3 1,305,095 - AMB_RS23260 0.24 -2.3 1,305,148 + AMB_RS23260 0.29 -1.9 1,305,148 + AMB_RS23260 0.29 +1.5 1,305,148 + AMB_RS23260 0.29 -2.1 1,305,148 + AMB_RS23260 0.29 -2.6 1,305,148 + AMB_RS23260 0.29 -2.9 1,305,149 - AMB_RS23260 0.29 +0.3 1,305,328 + AMB_RS23260 0.49 -0.1 1,305,328 + AMB_RS23260 0.49 +0.5 1,305,441 + AMB_RS23260 0.62 +1.5 1,305,441 + AMB_RS23260 0.62 +0.0 1,305,458 - AMB_RS23260 0.63 -1.1 1,305,496 + AMB_RS23260 0.68 +0.3 1,305,550 - AMB_RS23260 0.73 +0.1 1,305,625 + AMB_RS23260 0.82 -0.4 1,305,625 + AMB_RS23260 0.82 +0.1 1,305,625 + AMB_RS23260 0.82 +0.3 1,305,626 - AMB_RS23260 0.82 +0.0 1,305,640 + AMB_RS23260 0.83 -0.1 1,305,640 + AMB_RS23260 0.83 +0.5 1,305,640 + AMB_RS23260 0.83 -0.5 1,305,640 + AMB_RS23260 0.83 +0.5 1,305,641 - AMB_RS23260 0.83 +0.8 1,305,641 - AMB_RS23260 0.83 +0.2 1,305,727 + +0.1 1,305,727 + -1.5 1,305,742 + -0.5 1,305,742 + -2.4 1,305,742 + +0.3 1,305,743 - -0.9 1,305,743 - +0.2 1,305,752 + +0.4 1,305,752 + -0.3 1,305,753 - -2.4 1,305,753 - -0.2 1,305,806 - -2.6 1,305,807 + -0.8 1,305,807 + +0.1 1,305,807 + -0.2 1,305,807 + +0.5 1,305,807 + +1.0 1,305,807 + +1.5 1,305,807 + +1.4 1,305,807 + +0.3 1,305,807 + +0.4 1,305,808 - +0.7 1,305,808 - -0.1 1,305,808 - +0.1 1,305,808 - -0.8 1,305,808 - -0.1 1,305,808 - -2.6 1,305,808 - -0.2
Or see this region's nucleotide sequence