Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05800 and AMB_RS05805 overlap by 13 nucleotides AMB_RS05805 and AMB_RS05810 are separated by 529 nucleotides AMB_RS05810 and AMB_RS05815 are separated by 118 nucleotides AMB_RS05815 and AMB_RS05820 overlap by 7 nucleotides AMB_RS05820 and AMB_RS05825 overlap by 4 nucleotides
AMB_RS05800: AMB_RS05800 - hypothetical protein, at 1,226,144 to 1,228,240
_RS05800
AMB_RS05805: AMB_RS05805 - hypothetical protein, at 1,228,228 to 1,228,626
_RS05805
AMB_RS05810: AMB_RS05810 - hypothetical protein, at 1,229,156 to 1,229,548
_RS05810
AMB_RS05815: AMB_RS05815 - DNA-binding protein, at 1,229,667 to 1,229,918
_RS05815
AMB_RS05820: AMB_RS05820 - hypothetical protein, at 1,229,912 to 1,230,187
_RS05820
AMB_RS05825: AMB_RS05825 - hypothetical protein, at 1,230,184 to 1,230,663
_RS05825
Position (kb)
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1228.259 kb on + strand at 1228.259 kb on + strand at 1228.260 kb on - strand at 1228.322 kb on + strand, within AMB_RS05805 at 1228.322 kb on + strand, within AMB_RS05805 at 1228.322 kb on + strand, within AMB_RS05805 at 1228.322 kb on + strand, within AMB_RS05805 at 1228.322 kb on + strand, within AMB_RS05805 at 1228.322 kb on + strand, within AMB_RS05805 at 1228.435 kb on - strand, within AMB_RS05805 at 1228.602 kb on + strand at 1228.612 kb on - strand at 1228.783 kb on - strand at 1228.783 kb on - strand at 1228.784 kb on + strand at 1228.849 kb on + strand at 1228.850 kb on - strand at 1228.870 kb on + strand at 1228.932 kb on + strand at 1228.932 kb on + strand at 1228.933 kb on - strand at 1228.933 kb on - strand at 1229.087 kb on + strand at 1229.088 kb on - strand at 1229.088 kb on - strand at 1229.088 kb on - strand at 1229.091 kb on - strand at 1229.091 kb on - strand at 1229.222 kb on + strand, within AMB_RS05810 at 1229.222 kb on + strand, within AMB_RS05810 at 1229.222 kb on + strand, within AMB_RS05810 at 1229.222 kb on + strand, within AMB_RS05810 at 1229.222 kb on + strand, within AMB_RS05810 at 1229.222 kb on + strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.223 kb on - strand, within AMB_RS05810 at 1229.225 kb on - strand, within AMB_RS05810 at 1229.318 kb on + strand, within AMB_RS05810 at 1229.607 kb on + strand at 1229.608 kb on - strand at 1229.608 kb on - strand at 1229.683 kb on + strand at 1229.766 kb on + strand, within AMB_RS05815 at 1229.766 kb on + strand, within AMB_RS05815 at 1229.767 kb on - strand, within AMB_RS05815 at 1229.767 kb on - strand, within AMB_RS05815 at 1229.767 kb on - strand, within AMB_RS05815 at 1229.817 kb on + strand, within AMB_RS05815 at 1229.818 kb on - strand, within AMB_RS05815 at 1230.085 kb on - strand, within AMB_RS05820 at 1230.138 kb on - strand, within AMB_RS05820 at 1230.138 kb on - strand, within AMB_RS05820 at 1230.138 kb on - strand, within AMB_RS05820 at 1230.348 kb on + strand, within AMB_RS05825 at 1230.349 kb on - strand, within AMB_RS05825 at 1230.353 kb on - strand, within AMB_RS05825 at 1230.530 kb on - strand, within AMB_RS05825 at 1230.530 kb on - strand, within AMB_RS05825
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,228,259 + -0.3 1,228,259 + -1.6 1,228,260 - -0.1 1,228,322 + AMB_RS05805 0.24 -0.8 1,228,322 + AMB_RS05805 0.24 -0.1 1,228,322 + AMB_RS05805 0.24 -0.9 1,228,322 + AMB_RS05805 0.24 -0.4 1,228,322 + AMB_RS05805 0.24 +0.4 1,228,322 + AMB_RS05805 0.24 -0.3 1,228,435 - AMB_RS05805 0.52 -0.1 1,228,602 + +0.5 1,228,612 - -1.1 1,228,783 - -0.1 1,228,783 - -0.3 1,228,784 + -1.6 1,228,849 + -0.2 1,228,850 - -0.8 1,228,870 + -1.8 1,228,932 + -2.3 1,228,932 + +2.1 1,228,933 - +0.5 1,228,933 - -0.6 1,229,087 + -0.5 1,229,088 - +0.5 1,229,088 - -0.3 1,229,088 - +0.8 1,229,091 - -1.3 1,229,091 - -2.1 1,229,222 + AMB_RS05810 0.17 -0.1 1,229,222 + AMB_RS05810 0.17 -1.9 1,229,222 + AMB_RS05810 0.17 +0.4 1,229,222 + AMB_RS05810 0.17 -2.7 1,229,222 + AMB_RS05810 0.17 -0.8 1,229,222 + AMB_RS05810 0.17 -0.1 1,229,223 - AMB_RS05810 0.17 -1.3 1,229,223 - AMB_RS05810 0.17 -0.3 1,229,223 - AMB_RS05810 0.17 -1.9 1,229,223 - AMB_RS05810 0.17 -0.9 1,229,223 - AMB_RS05810 0.17 -1.3 1,229,223 - AMB_RS05810 0.17 -1.3 1,229,223 - AMB_RS05810 0.17 +1.5 1,229,223 - AMB_RS05810 0.17 -1.6 1,229,223 - AMB_RS05810 0.17 -0.2 1,229,223 - AMB_RS05810 0.17 -1.1 1,229,223 - AMB_RS05810 0.17 -0.0 1,229,223 - AMB_RS05810 0.17 -0.1 1,229,223 - AMB_RS05810 0.17 -2.2 1,229,223 - AMB_RS05810 0.17 -1.1 1,229,225 - AMB_RS05810 0.18 -1.6 1,229,318 + AMB_RS05810 0.41 +0.5 1,229,607 + -0.8 1,229,608 - -0.2 1,229,608 - -0.6 1,229,683 + -1.1 1,229,766 + AMB_RS05815 0.39 -1.6 1,229,766 + AMB_RS05815 0.39 +0.4 1,229,767 - AMB_RS05815 0.40 -0.1 1,229,767 - AMB_RS05815 0.40 +1.4 1,229,767 - AMB_RS05815 0.40 +0.5 1,229,817 + AMB_RS05815 0.60 -0.1 1,229,818 - AMB_RS05815 0.60 +0.5 1,230,085 - AMB_RS05820 0.63 -0.5 1,230,138 - AMB_RS05820 0.82 +0.2 1,230,138 - AMB_RS05820 0.82 -1.6 1,230,138 - AMB_RS05820 0.82 -3.4 1,230,348 + AMB_RS05825 0.34 -2.4 1,230,349 - AMB_RS05825 0.34 -0.3 1,230,353 - AMB_RS05825 0.35 -2.6 1,230,530 - AMB_RS05825 0.72 -1.8 1,230,530 - AMB_RS05825 0.72 +0.5
Or see this region's nucleotide sequence