Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05205 and AMB_RS05210 overlap by 17 nucleotides AMB_RS05210 and AMB_RS05215 are separated by 180 nucleotides AMB_RS05215 and AMB_RS05220 are separated by 271 nucleotides
AMB_RS05205: AMB_RS05205 - MFS transporter, at 1,075,660 to 1,077,594
_RS05205
AMB_RS05210: AMB_RS05210 - hypothetical protein, at 1,077,578 to 1,078,348
_RS05210
AMB_RS05215: AMB_RS05215 - methyl-accepting chemotaxis protein, at 1,078,529 to 1,079,698
_RS05215
AMB_RS05220: AMB_RS05220 - hypothetical protein, at 1,079,970 to 1,080,950
_RS05220
Position (kb)
1078
1079
1080 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1077.682 kb on - strand, within AMB_RS05210 at 1077.682 kb on - strand, within AMB_RS05210 at 1077.774 kb on + strand, within AMB_RS05210 at 1077.775 kb on - strand, within AMB_RS05210 at 1077.775 kb on - strand, within AMB_RS05210 at 1077.834 kb on + strand, within AMB_RS05210 at 1077.834 kb on + strand, within AMB_RS05210 at 1077.834 kb on + strand, within AMB_RS05210 at 1077.834 kb on + strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.835 kb on - strand, within AMB_RS05210 at 1077.894 kb on + strand, within AMB_RS05210 at 1077.895 kb on - strand, within AMB_RS05210 at 1078.270 kb on - strand, within AMB_RS05210 at 1078.384 kb on + strand at 1078.384 kb on + strand at 1078.384 kb on + strand at 1078.456 kb on + strand at 1078.459 kb on - strand at 1078.459 kb on - strand at 1078.497 kb on - strand at 1078.522 kb on + strand at 1078.522 kb on + strand at 1078.642 kb on + strand at 1078.827 kb on + strand, within AMB_RS05215 at 1079.566 kb on - strand, within AMB_RS05215 at 1079.730 kb on - strand at 1079.732 kb on - strand at 1079.732 kb on - strand at 1079.758 kb on - strand at 1079.878 kb on + strand at 1079.878 kb on + strand at 1079.895 kb on - strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.976 kb on + strand at 1079.977 kb on - strand at 1079.977 kb on - strand at 1080.657 kb on + strand, within AMB_RS05220 at 1080.657 kb on + strand, within AMB_RS05220
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 1,077,682 - AMB_RS05210 0.13 -0.8 1,077,682 - AMB_RS05210 0.13 -1.0 1,077,774 + AMB_RS05210 0.25 -2.1 1,077,775 - AMB_RS05210 0.26 +0.6 1,077,775 - AMB_RS05210 0.26 -0.3 1,077,834 + AMB_RS05210 0.33 +0.0 1,077,834 + AMB_RS05210 0.33 -3.7 1,077,834 + AMB_RS05210 0.33 -0.0 1,077,834 + AMB_RS05210 0.33 -1.9 1,077,835 - AMB_RS05210 0.33 -0.1 1,077,835 - AMB_RS05210 0.33 -1.8 1,077,835 - AMB_RS05210 0.33 -2.9 1,077,835 - AMB_RS05210 0.33 -0.4 1,077,835 - AMB_RS05210 0.33 +0.1 1,077,835 - AMB_RS05210 0.33 -0.4 1,077,894 + AMB_RS05210 0.41 +0.2 1,077,895 - AMB_RS05210 0.41 -1.0 1,078,270 - AMB_RS05210 0.90 -1.0 1,078,384 + +0.9 1,078,384 + -0.1 1,078,384 + +0.2 1,078,456 + -1.4 1,078,459 - +0.3 1,078,459 - +0.6 1,078,497 - +0.4 1,078,522 + -1.8 1,078,522 + -0.4 1,078,642 + -0.4 1,078,827 + AMB_RS05215 0.25 -0.1 1,079,566 - AMB_RS05215 0.89 -2.8 1,079,730 - -1.4 1,079,732 - +0.2 1,079,732 - +0.6 1,079,758 - +0.6 1,079,878 + -0.8 1,079,878 + -0.1 1,079,895 - -0.0 1,079,976 + +0.2 1,079,976 + -0.4 1,079,976 + -0.2 1,079,976 + -0.2 1,079,976 + -0.8 1,079,976 + -2.2 1,079,976 + +0.3 1,079,977 - -1.2 1,079,977 - -0.8 1,080,657 + AMB_RS05220 0.70 -0.4 1,080,657 + AMB_RS05220 0.70 -2.1
Or see this region's nucleotide sequence