Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04635 and AMB_RS04640 are separated by 256 nucleotides AMB_RS04640 and AMB_RS04645 are separated by 265 nucleotides AMB_RS04645 and AMB_RS04650 are separated by 245 nucleotides AMB_RS04650 and AMB_RS04655 are separated by 0 nucleotides
AMB_RS04635: AMB_RS04635 - site-specific integrase, at 970,341 to 971,309
_RS04635
AMB_RS04640: AMB_RS04640 - hypothetical protein, at 971,566 to 971,820
_RS04640
AMB_RS04645: AMB_RS04645 - ATP-dependent helicase, at 972,086 to 974,710
_RS04645
AMB_RS04650: AMB_RS04650 - hypothetical protein, at 974,956 to 975,345
_RS04650
AMB_RS04655: AMB_RS04655 - hypothetical protein, at 975,346 to 975,783
_RS04655
Position (kb) 972
973
974
975 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 971.241 kb on + strand at 971.242 kb on - strand at 971.252 kb on - strand at 971.339 kb on + strand at 971.351 kb on + strand at 971.352 kb on - strand at 971.385 kb on + strand at 971.418 kb on + strand at 971.419 kb on - strand at 971.456 kb on + strand at 971.456 kb on + strand at 971.457 kb on - strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.566 kb on - strand at 971.566 kb on - strand at 971.708 kb on + strand, within AMB_RS04640 at 971.708 kb on + strand, within AMB_RS04640 at 971.818 kb on + strand at 971.819 kb on - strand at 971.819 kb on - strand at 971.960 kb on - strand at 972.073 kb on + strand at 972.136 kb on + strand at 972.136 kb on + strand at 972.203 kb on - strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.254 kb on - strand at 972.254 kb on - strand at 972.254 kb on - strand at 972.298 kb on + strand at 972.298 kb on + strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.314 kb on - strand at 972.418 kb on + strand, within AMB_RS04645 at 972.484 kb on + strand, within AMB_RS04645 at 972.484 kb on + strand, within AMB_RS04645 at 972.485 kb on - strand, within AMB_RS04645 at 972.513 kb on + strand, within AMB_RS04645 at 972.513 kb on + strand, within AMB_RS04645 at 972.514 kb on - strand, within AMB_RS04645 at 972.514 kb on - strand, within AMB_RS04645 at 972.748 kb on + strand, within AMB_RS04645 at 972.751 kb on + strand, within AMB_RS04645 at 972.751 kb on + strand, within AMB_RS04645 at 972.779 kb on + strand, within AMB_RS04645 at 972.779 kb on + strand, within AMB_RS04645 at 972.779 kb on + strand, within AMB_RS04645 at 972.779 kb on + strand, within AMB_RS04645 at 972.780 kb on - strand, within AMB_RS04645 at 972.780 kb on - strand, within AMB_RS04645 at 972.780 kb on - strand, within AMB_RS04645 at 972.897 kb on + strand, within AMB_RS04645 at 972.897 kb on + strand, within AMB_RS04645 at 972.897 kb on + strand, within AMB_RS04645 at 972.897 kb on + strand, within AMB_RS04645 at 972.897 kb on + strand, within AMB_RS04645 at 972.898 kb on - strand, within AMB_RS04645 at 973.036 kb on + strand, within AMB_RS04645 at 973.069 kb on - strand, within AMB_RS04645 at 973.069 kb on - strand, within AMB_RS04645 at 973.069 kb on - strand, within AMB_RS04645 at 973.071 kb on + strand, within AMB_RS04645 at 973.071 kb on + strand, within AMB_RS04645 at 973.071 kb on + strand, within AMB_RS04645 at 973.072 kb on - strand, within AMB_RS04645 at 973.195 kb on + strand, within AMB_RS04645 at 973.195 kb on + strand, within AMB_RS04645 at 973.195 kb on + strand, within AMB_RS04645 at 973.195 kb on + strand, within AMB_RS04645 at 973.196 kb on - strand, within AMB_RS04645 at 973.196 kb on - strand, within AMB_RS04645 at 973.210 kb on + strand, within AMB_RS04645 at 973.211 kb on - strand, within AMB_RS04645 at 973.258 kb on + strand, within AMB_RS04645 at 973.258 kb on + strand, within AMB_RS04645 at 973.259 kb on - strand, within AMB_RS04645 at 973.259 kb on - strand, within AMB_RS04645 at 973.259 kb on - strand, within AMB_RS04645 at 973.327 kb on + strand, within AMB_RS04645 at 973.327 kb on + strand, within AMB_RS04645 at 973.327 kb on + strand, within AMB_RS04645 at 973.331 kb on + strand, within AMB_RS04645 at 973.331 kb on + strand, within AMB_RS04645 at 973.331 kb on + strand, within AMB_RS04645 at 973.332 kb on - strand, within AMB_RS04645 at 973.343 kb on - strand, within AMB_RS04645 at 973.343 kb on - strand, within AMB_RS04645 at 973.343 kb on - strand, within AMB_RS04645 at 973.407 kb on - strand, within AMB_RS04645 at 973.625 kb on + strand, within AMB_RS04645 at 973.626 kb on - strand, within AMB_RS04645 at 973.626 kb on - strand, within AMB_RS04645 at 973.790 kb on + strand, within AMB_RS04645 at 973.891 kb on - strand, within AMB_RS04645 at 973.946 kb on + strand, within AMB_RS04645 at 973.946 kb on + strand, within AMB_RS04645 at 973.947 kb on - strand, within AMB_RS04645 at 974.051 kb on - strand, within AMB_RS04645 at 974.484 kb on + strand at 974.560 kb on + strand at 974.561 kb on - strand at 974.587 kb on + strand at 974.588 kb on - strand at 974.632 kb on + strand at 974.632 kb on + strand at 974.675 kb on + strand at 974.675 kb on + strand at 974.675 kb on + strand at 974.726 kb on - strand at 974.726 kb on - strand at 974.726 kb on - strand at 974.835 kb on + strand at 974.836 kb on - strand at 974.836 kb on - strand at 974.836 kb on - strand at 974.876 kb on + strand at 974.891 kb on + strand at 974.891 kb on + strand at 974.892 kb on - strand at 974.892 kb on - strand at 974.938 kb on - strand at 975.047 kb on + strand, within AMB_RS04650 at 975.145 kb on - strand, within AMB_RS04650 at 975.264 kb on + strand, within AMB_RS04650 at 975.357 kb on - strand at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.607 kb on + strand, within AMB_RS04655 at 975.608 kb on - strand, within AMB_RS04655 at 975.682 kb on + strand, within AMB_RS04655
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 971,241 + -1.8 971,242 - +0.2 971,252 - -1.0 971,339 + -1.8 971,351 + -0.4 971,352 - +0.5 971,385 + -0.1 971,418 + -0.0 971,419 - +1.3 971,456 + +0.2 971,456 + -0.8 971,457 - -0.4 971,565 + -0.2 971,565 + -0.6 971,565 + -0.0 971,565 + -1.4 971,566 - -0.6 971,566 - -0.0 971,708 + AMB_RS04640 0.56 +0.2 971,708 + AMB_RS04640 0.56 -0.1 971,818 + +0.6 971,819 - -1.6 971,819 - -1.8 971,960 - +0.2 972,073 + -0.4 972,136 + -0.4 972,136 + -0.3 972,203 - -0.8 972,253 + -0.2 972,253 + -0.3 972,253 + -1.4 972,253 + -0.6 972,253 + -1.1 972,253 + -0.0 972,253 + -0.3 972,254 - -0.1 972,254 - +1.6 972,254 - +0.7 972,298 + -0.0 972,298 + +1.6 972,299 - +0.0 972,299 - -0.2 972,299 - +0.1 972,299 - -0.3 972,299 - -3.0 972,299 - +0.1 972,299 - -0.1 972,314 - -1.6 972,418 + AMB_RS04645 0.13 +0.8 972,484 + AMB_RS04645 0.15 -0.5 972,484 + AMB_RS04645 0.15 -0.8 972,485 - AMB_RS04645 0.15 +0.4 972,513 + AMB_RS04645 0.16 +0.2 972,513 + AMB_RS04645 0.16 -1.0 972,514 - AMB_RS04645 0.16 -0.2 972,514 - AMB_RS04645 0.16 +1.6 972,748 + AMB_RS04645 0.25 +1.6 972,751 + AMB_RS04645 0.25 -1.3 972,751 + AMB_RS04645 0.25 -0.0 972,779 + AMB_RS04645 0.26 +0.1 972,779 + AMB_RS04645 0.26 +0.1 972,779 + AMB_RS04645 0.26 +1.6 972,779 + AMB_RS04645 0.26 -0.4 972,780 - AMB_RS04645 0.26 +0.0 972,780 - AMB_RS04645 0.26 -0.1 972,780 - AMB_RS04645 0.26 +0.1 972,897 + AMB_RS04645 0.31 +0.6 972,897 + AMB_RS04645 0.31 -0.7 972,897 + AMB_RS04645 0.31 +0.2 972,897 + AMB_RS04645 0.31 +0.1 972,897 + AMB_RS04645 0.31 -1.8 972,898 - AMB_RS04645 0.31 -0.3 973,036 + AMB_RS04645 0.36 -0.3 973,069 - AMB_RS04645 0.37 -1.4 973,069 - AMB_RS04645 0.37 +0.3 973,069 - AMB_RS04645 0.37 -2.3 973,071 + AMB_RS04645 0.38 -0.4 973,071 + AMB_RS04645 0.38 -1.0 973,071 + AMB_RS04645 0.38 -2.7 973,072 - AMB_RS04645 0.38 +0.1 973,195 + AMB_RS04645 0.42 -1.0 973,195 + AMB_RS04645 0.42 +0.1 973,195 + AMB_RS04645 0.42 -0.4 973,195 + AMB_RS04645 0.42 -0.1 973,196 - AMB_RS04645 0.42 -0.3 973,196 - AMB_RS04645 0.42 -0.5 973,210 + AMB_RS04645 0.43 +0.6 973,211 - AMB_RS04645 0.43 +0.6 973,258 + AMB_RS04645 0.45 -0.7 973,258 + AMB_RS04645 0.45 +0.0 973,259 - AMB_RS04645 0.45 -2.0 973,259 - AMB_RS04645 0.45 +0.3 973,259 - AMB_RS04645 0.45 -1.9 973,327 + AMB_RS04645 0.47 +0.7 973,327 + AMB_RS04645 0.47 -0.1 973,327 + AMB_RS04645 0.47 +0.5 973,331 + AMB_RS04645 0.47 +0.1 973,331 + AMB_RS04645 0.47 +0.6 973,331 + AMB_RS04645 0.47 -1.0 973,332 - AMB_RS04645 0.47 -0.8 973,343 - AMB_RS04645 0.48 +1.6 973,343 - AMB_RS04645 0.48 -0.3 973,343 - AMB_RS04645 0.48 -1.3 973,407 - AMB_RS04645 0.50 +0.0 973,625 + AMB_RS04645 0.59 +1.6 973,626 - AMB_RS04645 0.59 -0.6 973,626 - AMB_RS04645 0.59 -0.4 973,790 + AMB_RS04645 0.65 -3.1 973,891 - AMB_RS04645 0.69 -0.1 973,946 + AMB_RS04645 0.71 -0.4 973,946 + AMB_RS04645 0.71 -3.0 973,947 - AMB_RS04645 0.71 +1.6 974,051 - AMB_RS04645 0.75 +0.1 974,484 + +0.1 974,560 + -0.5 974,561 - -0.0 974,587 + -0.1 974,588 - +0.1 974,632 + -1.3 974,632 + -1.0 974,675 + -0.1 974,675 + -0.2 974,675 + +0.0 974,726 - -0.4 974,726 - -0.2 974,726 - -0.6 974,835 + -0.4 974,836 - +0.0 974,836 - -0.4 974,836 - -0.7 974,876 + -0.0 974,891 + -2.1 974,891 + +1.6 974,892 - -2.1 974,892 - +0.8 974,938 - -0.0 975,047 + AMB_RS04650 0.23 -1.0 975,145 - AMB_RS04650 0.48 -2.4 975,264 + AMB_RS04650 0.79 -0.1 975,357 - -0.0 975,607 + AMB_RS04655 0.60 +0.5 975,607 + AMB_RS04655 0.60 +0.4 975,607 + AMB_RS04655 0.60 -0.4 975,607 + AMB_RS04655 0.60 +0.1 975,607 + AMB_RS04655 0.60 +0.0 975,607 + AMB_RS04655 0.60 -1.3 975,607 + AMB_RS04655 0.60 +1.0 975,608 - AMB_RS04655 0.60 -1.0 975,682 + AMB_RS04655 0.77 -1.3
Or see this region's nucleotide sequence