Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04290 and AMB_RS04295 are separated by 137 nucleotides AMB_RS04295 and AMB_RS04300 are separated by 143 nucleotides
AMB_RS04290: AMB_RS04290 - poly-beta-hydroxybutyrate polymerase, at 900,526 to 901,632
_RS04290
AMB_RS04295: AMB_RS04295 - acetyl-CoA C-acetyltransferase, at 901,770 to 902,942
_RS04295
AMB_RS04300: AMB_RS04300 - beta-ketoacyl-ACP reductase, at 903,086 to 903,808
_RS04300
Position (kb)
901
902
903 Strain fitness (log2 ratio)
-2
-1
0
1 at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.742 kb on + strand at 901.743 kb on - strand at 901.744 kb on + strand at 901.744 kb on + strand at 901.744 kb on + strand at 901.745 kb on - strand at 902.145 kb on + strand, within AMB_RS04295 at 902.945 kb on + strand at 902.945 kb on + strand at 902.946 kb on - strand at 902.946 kb on - strand at 902.959 kb on - strand at 903.803 kb on + strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.939 kb on - strand at 903.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 901,630 + -1.5 901,630 + -0.5 901,630 + +1.5 901,630 + -1.9 901,630 + +1.5 901,630 + -0.8 901,630 + -0.5 901,742 + -0.1 901,743 - -0.2 901,744 + +0.3 901,744 + -0.0 901,744 + -0.1 901,745 - -2.3 902,145 + AMB_RS04295 0.32 -1.3 902,945 + -1.8 902,945 + +0.3 902,946 - -0.8 902,946 - -0.8 902,959 - -1.0 903,803 + +0.1 903,804 - -0.0 903,804 - -0.8 903,804 - -0.5 903,804 - +0.2 903,804 - -0.4 903,938 + +0.2 903,938 + +0.6 903,938 + -0.8 903,938 + +0.5 903,939 - +0.2 903,939 - -0.1
Or see this region's nucleotide sequence