Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04150 and AMB_RS04155 are separated by 126 nucleotides AMB_RS04155 and AMB_RS04160 overlap by 4 nucleotides AMB_RS04160 and AMB_RS04165 are separated by 133 nucleotides AMB_RS04165 and AMB_RS04170 are separated by 133 nucleotides
AMB_RS04150: AMB_RS04150 - acetylornithine transaminase, at 869,137 to 870,303
_RS04150
AMB_RS04155: AMB_RS04155 - heme-binding protein, at 870,430 to 870,822
_RS04155
AMB_RS04160: AMB_RS04160 - NAD-dependent succinate-semialdehyde dehydrogenase, at 870,819 to 872,318
_RS04160
AMB_RS04165: AMB_RS04165 - homoprotocatechuate degradation operon regulator HpaR, at 872,452 to 872,919
_RS04165
AMB_RS04170: AMB_RS04170 - CoA transferase, at 873,053 to 874,264
_RS04170
Position (kb) 870
871
872
873 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 870.156 kb on + strand, within AMB_RS04150 at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 871.024 kb on + strand, within AMB_RS04160 at 871.369 kb on + strand, within AMB_RS04160 at 871.370 kb on - strand, within AMB_RS04160 at 871.370 kb on - strand, within AMB_RS04160 at 871.370 kb on - strand, within AMB_RS04160 at 871.370 kb on - strand, within AMB_RS04160 at 871.429 kb on + strand, within AMB_RS04160 at 871.897 kb on + strand, within AMB_RS04160 at 871.898 kb on - strand, within AMB_RS04160 at 871.898 kb on - strand, within AMB_RS04160 at 871.945 kb on + strand, within AMB_RS04160 at 871.945 kb on + strand, within AMB_RS04160 at 871.946 kb on - strand, within AMB_RS04160 at 872.236 kb on + strand at 872.236 kb on + strand at 872.236 kb on + strand at 872.236 kb on + strand at 872.237 kb on - strand at 872.237 kb on - strand at 872.237 kb on - strand at 872.237 kb on - strand at 872.237 kb on - strand at 872.342 kb on + strand at 872.342 kb on + strand at 872.343 kb on - strand at 872.343 kb on - strand at 872.343 kb on - strand at 872.381 kb on + strand at 872.381 kb on + strand at 872.381 kb on + strand at 872.382 kb on - strand at 872.382 kb on - strand at 872.917 kb on + strand at 872.918 kb on - strand at 872.918 kb on - strand at 872.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 870,156 + AMB_RS04150 0.87 -0.0 870,844 + -1.0 870,844 + -1.5 870,844 + +0.5 870,844 + -0.5 870,844 + -3.6 870,844 + -0.5 870,844 + +0.5 870,844 + -1.8 870,845 - -1.3 870,845 - +0.5 870,845 - +0.1 870,845 - -1.2 870,845 - +0.2 870,845 - -1.5 870,845 - +0.8 871,024 + AMB_RS04160 0.14 -0.3 871,369 + AMB_RS04160 0.37 -3.3 871,370 - AMB_RS04160 0.37 -0.2 871,370 - AMB_RS04160 0.37 +0.5 871,370 - AMB_RS04160 0.37 +1.1 871,370 - AMB_RS04160 0.37 -0.8 871,429 + AMB_RS04160 0.41 +0.1 871,897 + AMB_RS04160 0.72 -0.1 871,898 - AMB_RS04160 0.72 -1.3 871,898 - AMB_RS04160 0.72 -0.1 871,945 + AMB_RS04160 0.75 -1.7 871,945 + AMB_RS04160 0.75 -2.4 871,946 - AMB_RS04160 0.75 -0.0 872,236 + -1.6 872,236 + -3.4 872,236 + +0.2 872,236 + +0.5 872,237 - -0.0 872,237 - -2.3 872,237 - +0.5 872,237 - -1.3 872,237 - +0.5 872,342 + -0.8 872,342 + +0.2 872,343 - -0.0 872,343 - -1.9 872,343 - +0.4 872,381 + +0.7 872,381 + -1.3 872,381 + +0.4 872,382 - +0.2 872,382 - +0.9 872,917 + +0.0 872,918 - -1.3 872,918 - +0.6 872,918 - -1.3
Or see this region's nucleotide sequence