Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04010 and AMB_RS04015 are separated by 244 nucleotides AMB_RS04015 and AMB_RS04020 are separated by 102 nucleotides AMB_RS04020 and AMB_RS04025 are separated by 120 nucleotides
AMB_RS04010: AMB_RS04010 - putative heme iron utilization protein, at 839,901 to 840,788
_RS04010
AMB_RS04015: AMB_RS04015 - phosphoenolpyruvate carboxykinase (GTP), at 841,033 to 842,886
_RS04015
AMB_RS04020: AMB_RS04020 - hypothetical protein, at 842,989 to 843,423
_RS04020
AMB_RS04025: AMB_RS04025 - glutathione S-transferase, at 843,544 to 844,149
_RS04025
Position (kb) 841
842
843 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 840.177 kb on + strand, within AMB_RS04010 at 840.177 kb on + strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.420 kb on + strand, within AMB_RS04010 at 840.420 kb on + strand, within AMB_RS04010 at 840.421 kb on - strand, within AMB_RS04010 at 840.926 kb on + strand at 842.952 kb on + strand at 842.952 kb on + strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.958 kb on + strand at 842.991 kb on - strand at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.418 kb on + strand at 843.419 kb on - strand at 843.419 kb on - strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.556 kb on + strand at 843.557 kb on - strand at 843.575 kb on - strand at 843.575 kb on - strand at 843.755 kb on - strand, within AMB_RS04025
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 840,177 + AMB_RS04010 0.31 -0.2 840,177 + AMB_RS04010 0.31 -0.1 840,178 - AMB_RS04010 0.31 -0.7 840,178 - AMB_RS04010 0.31 -0.6 840,178 - AMB_RS04010 0.31 -0.8 840,178 - AMB_RS04010 0.31 -0.5 840,420 + AMB_RS04010 0.58 -0.8 840,420 + AMB_RS04010 0.58 +0.4 840,421 - AMB_RS04010 0.59 -1.9 840,926 + -2.2 842,952 + -0.5 842,952 + -1.8 842,953 - +0.1 842,953 - +0.8 842,953 - -1.6 842,953 - +0.2 842,953 - -1.1 842,958 + -1.1 842,991 - -1.3 843,215 + AMB_RS04020 0.52 -0.1 843,215 + AMB_RS04020 0.52 +0.6 843,215 + AMB_RS04020 0.52 -2.1 843,215 + AMB_RS04020 0.52 +0.5 843,215 + AMB_RS04020 0.52 -1.1 843,215 + AMB_RS04020 0.52 -1.3 843,215 + AMB_RS04020 0.52 -1.8 843,215 + AMB_RS04020 0.52 -0.8 843,216 - AMB_RS04020 0.52 -2.8 843,216 - AMB_RS04020 0.52 +0.1 843,216 - AMB_RS04020 0.52 +0.2 843,216 - AMB_RS04020 0.52 -1.1 843,216 - AMB_RS04020 0.52 +0.4 843,216 - AMB_RS04020 0.52 +1.5 843,216 - AMB_RS04020 0.52 +0.3 843,216 - AMB_RS04020 0.52 -0.5 843,418 + +1.2 843,419 - +0.9 843,419 - +0.1 843,465 + -0.2 843,465 + -0.4 843,465 + -0.2 843,465 + +0.6 843,465 + -0.1 843,465 + +0.5 843,465 + -2.4 843,465 + -0.8 843,466 - -0.1 843,466 - -0.1 843,466 - +0.1 843,466 - -0.1 843,466 - -3.7 843,556 + +0.0 843,557 - +0.2 843,575 - +0.1 843,575 - -1.6 843,755 - AMB_RS04025 0.35 -0.3
Or see this region's nucleotide sequence