Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03295 and AMB_RS03300 are separated by 408 nucleotides AMB_RS03300 and AMB_RS03305 are separated by 8 nucleotides AMB_RS03305 and AMB_RS03310 are separated by 375 nucleotides
AMB_RS03295: AMB_RS03295 - hypothetical protein, at 690,338 to 690,799
_RS03295
AMB_RS03300: AMB_RS03300 - transposase, at 691,208 to 691,501
_RS03300
AMB_RS03305: AMB_RS03305 - IS3 family transposase, at 691,510 to 692,355
_RS03305
AMB_RS03310: AMB_RS03310 - hypothetical protein, at 692,731 to 694,056
_RS03310
Position (kb) 691
692
693 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 690.515 kb on + strand, within AMB_RS03295 at 690.516 kb on - strand, within AMB_RS03295 at 690.557 kb on - strand, within AMB_RS03295 at 690.663 kb on - strand, within AMB_RS03295 at 690.687 kb on + strand, within AMB_RS03295 at 690.771 kb on + strand at 690.771 kb on + strand at 690.784 kb on + strand at 690.784 kb on + strand at 690.785 kb on - strand at 690.864 kb on - strand at 690.880 kb on + strand at 690.936 kb on - strand at 690.940 kb on - strand at 690.958 kb on + strand at 690.959 kb on - strand at 691.025 kb on - strand at 691.025 kb on - strand at 691.057 kb on + strand at 691.057 kb on + strand at 691.058 kb on - strand at 691.121 kb on - strand at 691.134 kb on - strand at 691.134 kb on - strand at 691.165 kb on - strand at 692.388 kb on + strand at 692.388 kb on + strand at 692.388 kb on + strand at 692.388 kb on + strand at 692.388 kb on + strand at 692.388 kb on + strand at 692.427 kb on - strand at 692.427 kb on - strand at 692.427 kb on - strand at 692.511 kb on - strand at 692.524 kb on + strand at 692.524 kb on + strand at 692.524 kb on + strand at 692.524 kb on + strand at 692.525 kb on - strand at 692.577 kb on + strand at 692.578 kb on - strand at 692.732 kb on + strand at 692.733 kb on - strand at 692.897 kb on + strand, within AMB_RS03310 at 692.897 kb on + strand, within AMB_RS03310 at 692.897 kb on + strand, within AMB_RS03310 at 692.918 kb on - strand, within AMB_RS03310 at 692.918 kb on - strand, within AMB_RS03310 at 692.943 kb on + strand, within AMB_RS03310 at 692.944 kb on - strand, within AMB_RS03310 at 692.983 kb on + strand, within AMB_RS03310 at 692.983 kb on + strand, within AMB_RS03310 at 693.049 kb on + strand, within AMB_RS03310 at 693.049 kb on + strand, within AMB_RS03310 at 693.050 kb on - strand, within AMB_RS03310 at 693.050 kb on - strand, within AMB_RS03310 at 693.117 kb on + strand, within AMB_RS03310 at 693.117 kb on + strand, within AMB_RS03310 at 693.118 kb on - strand, within AMB_RS03310 at 693.120 kb on + strand, within AMB_RS03310 at 693.121 kb on - strand, within AMB_RS03310 at 693.131 kb on - strand, within AMB_RS03310 at 693.134 kb on - strand, within AMB_RS03310 at 693.276 kb on + strand, within AMB_RS03310 at 693.276 kb on + strand, within AMB_RS03310 at 693.276 kb on + strand, within AMB_RS03310 at 693.277 kb on - strand, within AMB_RS03310 at 693.277 kb on - strand, within AMB_RS03310
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 690,515 + AMB_RS03295 0.38 -1.4 690,516 - AMB_RS03295 0.39 -1.4 690,557 - AMB_RS03295 0.47 +0.2 690,663 - AMB_RS03295 0.70 -0.2 690,687 + AMB_RS03295 0.76 -0.2 690,771 + -1.0 690,771 + -0.2 690,784 + -1.0 690,784 + +0.1 690,785 - -0.2 690,864 - -1.1 690,880 + -2.0 690,936 - -0.2 690,940 - -1.0 690,958 + +0.6 690,959 - -2.2 691,025 - -0.1 691,025 - +0.3 691,057 + +0.2 691,057 + -1.6 691,058 - -0.4 691,121 - -1.1 691,134 - -1.4 691,134 - +0.9 691,165 - -1.6 692,388 + -0.0 692,388 + +0.4 692,388 + -0.1 692,388 + -0.6 692,388 + -1.3 692,388 + -0.7 692,427 - -0.0 692,427 - +0.6 692,427 - +0.4 692,511 - +0.4 692,524 + +0.1 692,524 + +1.1 692,524 + -2.2 692,524 + -1.1 692,525 - -1.4 692,577 + -1.0 692,578 - +0.8 692,732 + +0.6 692,733 - -0.1 692,897 + AMB_RS03310 0.13 -0.2 692,897 + AMB_RS03310 0.13 -1.6 692,897 + AMB_RS03310 0.13 -0.1 692,918 - AMB_RS03310 0.14 -0.0 692,918 - AMB_RS03310 0.14 -0.4 692,943 + AMB_RS03310 0.16 -1.7 692,944 - AMB_RS03310 0.16 -0.8 692,983 + AMB_RS03310 0.19 -3.1 692,983 + AMB_RS03310 0.19 -2.6 693,049 + AMB_RS03310 0.24 +0.4 693,049 + AMB_RS03310 0.24 -0.1 693,050 - AMB_RS03310 0.24 -0.0 693,050 - AMB_RS03310 0.24 -2.8 693,117 + AMB_RS03310 0.29 -1.3 693,117 + AMB_RS03310 0.29 +0.2 693,118 - AMB_RS03310 0.29 -1.4 693,120 + AMB_RS03310 0.29 -0.3 693,121 - AMB_RS03310 0.29 +0.2 693,131 - AMB_RS03310 0.30 -0.4 693,134 - AMB_RS03310 0.30 -0.4 693,276 + AMB_RS03310 0.41 -1.9 693,276 + AMB_RS03310 0.41 +0.0 693,276 + AMB_RS03310 0.41 -0.0 693,277 - AMB_RS03310 0.41 +0.5 693,277 - AMB_RS03310 0.41 -1.1
Or see this region's nucleotide sequence