Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03265 and AMB_RS03270 are separated by 115 nucleotides AMB_RS03270 and AMB_RS03275 are separated by 503 nucleotides AMB_RS03275 and AMB_RS03280 are separated by 104 nucleotides
AMB_RS03265: AMB_RS03265 - enoyl-CoA hydratase, at 682,180 to 682,956
_RS03265
AMB_RS03270: AMB_RS03270 - 30S ribosomal protein S20, at 683,072 to 683,344
_RS03270
AMB_RS03275: AMB_RS03275 - chromosomal replication initiator protein DnaA, at 683,848 to 685,248
_RS03275
AMB_RS03280: AMB_RS03280 - DNA polymerase III subunit beta, at 685,353 to 686,471
_RS03280
Position (kb) 683
684
685
686 Strain fitness (log2 ratio) -2
-1
0
1 at 683.025 kb on + strand at 683.342 kb on + strand at 683.356 kb on + strand at 683.356 kb on + strand at 683.356 kb on + strand at 683.357 kb on - strand at 683.359 kb on + strand at 683.676 kb on + strand at 685.290 kb on + strand at 685.290 kb on + strand at 685.290 kb on + strand at 685.292 kb on + strand at 685.292 kb on + strand at 685.292 kb on + strand at 685.292 kb on + strand at 685.292 kb on + strand at 685.292 kb on + strand at 685.321 kb on + strand at 685.331 kb on + strand at 685.331 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 683,025 + -1.2 683,342 + +0.5 683,356 + -1.0 683,356 + -0.5 683,356 + +0.7 683,357 - -0.0 683,359 + -0.4 683,676 + -0.7 685,290 + +0.6 685,290 + -0.5 685,290 + -0.7 685,292 + -1.9 685,292 + +1.6 685,292 + +0.0 685,292 + -0.5 685,292 + -0.4 685,292 + -0.1 685,321 + +0.1 685,331 + -1.9 685,331 + -0.5
Or see this region's nucleotide sequence