Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03085 and AMB_RS03090 are separated by 124 nucleotides AMB_RS03090 and AMB_RS03095 are separated by 108 nucleotides AMB_RS03095 and AMB_RS03100 overlap by 4 nucleotides
AMB_RS03085: AMB_RS03085 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 644,939 to 645,976
_RS03085
AMB_RS03090: AMB_RS03090 - tRNA guanosine(34) transglycosylase Tgt, at 646,101 to 647,228
_RS03090
AMB_RS03095: AMB_RS03095 - tetratricopeptide repeat protein, at 647,337 to 647,840
_RS03095
AMB_RS03100: AMB_RS03100 - isoprenylcysteine carboxylmethyltransferase family protein, at 647,837 to 648,454
_RS03100
Position (kb) 646
647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 645.386 kb on + strand, within AMB_RS03085 at 645.386 kb on + strand, within AMB_RS03085 at 645.387 kb on - strand, within AMB_RS03085 at 645.387 kb on - strand, within AMB_RS03085 at 645.387 kb on - strand, within AMB_RS03085 at 645.387 kb on - strand, within AMB_RS03085 at 645.387 kb on - strand, within AMB_RS03085 at 645.388 kb on + strand, within AMB_RS03085 at 645.428 kb on + strand, within AMB_RS03085 at 645.428 kb on + strand, within AMB_RS03085 at 645.428 kb on + strand, within AMB_RS03085 at 645.899 kb on + strand at 645.900 kb on - strand at 645.924 kb on - strand at 645.932 kb on + strand at 645.932 kb on + strand at 645.932 kb on + strand at 645.933 kb on - strand at 645.938 kb on + strand at 645.938 kb on + strand at 645.972 kb on + strand at 645.972 kb on + strand at 645.972 kb on + strand at 645.972 kb on + strand at 645.973 kb on - strand at 645.986 kb on + strand at 645.987 kb on - strand at 645.987 kb on - strand at 646.284 kb on + strand, within AMB_RS03090 at 646.284 kb on + strand, within AMB_RS03090 at 646.284 kb on + strand, within AMB_RS03090 at 646.285 kb on - strand, within AMB_RS03090 at 646.285 kb on - strand, within AMB_RS03090 at 646.644 kb on + strand, within AMB_RS03090 at 646.677 kb on + strand, within AMB_RS03090 at 646.735 kb on - strand, within AMB_RS03090 at 647.046 kb on + strand, within AMB_RS03090 at 647.213 kb on + strand at 647.508 kb on + strand, within AMB_RS03095 at 647.508 kb on + strand, within AMB_RS03095 at 647.509 kb on - strand, within AMB_RS03095 at 647.509 kb on - strand, within AMB_RS03095 at 647.514 kb on + strand, within AMB_RS03095 at 647.514 kb on + strand, within AMB_RS03095 at 647.515 kb on - strand, within AMB_RS03095 at 647.697 kb on + strand, within AMB_RS03095 at 647.697 kb on + strand, within AMB_RS03095 at 647.855 kb on + strand at 647.855 kb on + strand at 647.855 kb on + strand at 647.856 kb on - strand at 647.856 kb on - strand at 647.856 kb on - strand at 647.939 kb on + strand, within AMB_RS03100 at 647.939 kb on + strand, within AMB_RS03100 at 647.939 kb on + strand, within AMB_RS03100 at 647.939 kb on + strand, within AMB_RS03100 at 647.939 kb on + strand, within AMB_RS03100 at 647.940 kb on - strand, within AMB_RS03100 at 647.940 kb on - strand, within AMB_RS03100 at 647.940 kb on - strand, within AMB_RS03100 at 647.940 kb on - strand, within AMB_RS03100 at 647.947 kb on + strand, within AMB_RS03100 at 648.095 kb on + strand, within AMB_RS03100 at 648.095 kb on + strand, within AMB_RS03100 at 648.099 kb on - strand, within AMB_RS03100 at 648.099 kb on - strand, within AMB_RS03100 at 648.099 kb on - strand, within AMB_RS03100 at 648.206 kb on + strand, within AMB_RS03100 at 648.207 kb on - strand, within AMB_RS03100 at 648.207 kb on - strand, within AMB_RS03100
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 645,386 + AMB_RS03085 0.43 +0.2 645,386 + AMB_RS03085 0.43 +0.0 645,387 - AMB_RS03085 0.43 +0.4 645,387 - AMB_RS03085 0.43 +0.9 645,387 - AMB_RS03085 0.43 +1.0 645,387 - AMB_RS03085 0.43 -0.4 645,387 - AMB_RS03085 0.43 -1.4 645,388 + AMB_RS03085 0.43 -0.2 645,428 + AMB_RS03085 0.47 +0.8 645,428 + AMB_RS03085 0.47 +0.6 645,428 + AMB_RS03085 0.47 -0.3 645,899 + -0.3 645,900 - +0.1 645,924 - +0.4 645,932 + -0.9 645,932 + +1.6 645,932 + -0.7 645,933 - +0.2 645,938 + -1.0 645,938 + +0.2 645,972 + -0.5 645,972 + +0.4 645,972 + -1.0 645,972 + -0.4 645,973 - -0.0 645,986 + +0.7 645,987 - -0.4 645,987 - -1.7 646,284 + AMB_RS03090 0.16 +0.8 646,284 + AMB_RS03090 0.16 -0.4 646,284 + AMB_RS03090 0.16 -0.4 646,285 - AMB_RS03090 0.16 -0.1 646,285 - AMB_RS03090 0.16 -0.4 646,644 + AMB_RS03090 0.48 +0.2 646,677 + AMB_RS03090 0.51 -1.2 646,735 - AMB_RS03090 0.56 -0.5 647,046 + AMB_RS03090 0.84 -1.2 647,213 + +0.6 647,508 + AMB_RS03095 0.34 +0.4 647,508 + AMB_RS03095 0.34 +0.6 647,509 - AMB_RS03095 0.34 +0.0 647,509 - AMB_RS03095 0.34 +0.6 647,514 + AMB_RS03095 0.35 +0.8 647,514 + AMB_RS03095 0.35 -3.1 647,515 - AMB_RS03095 0.35 -0.4 647,697 + AMB_RS03095 0.71 -1.4 647,697 + AMB_RS03095 0.71 +0.2 647,855 + +0.0 647,855 + -2.4 647,855 + +0.4 647,856 - -0.1 647,856 - +1.6 647,856 - -0.2 647,939 + AMB_RS03100 0.17 -1.0 647,939 + AMB_RS03100 0.17 -1.7 647,939 + AMB_RS03100 0.17 -1.0 647,939 + AMB_RS03100 0.17 +0.1 647,939 + AMB_RS03100 0.17 -1.4 647,940 - AMB_RS03100 0.17 -2.1 647,940 - AMB_RS03100 0.17 -1.0 647,940 - AMB_RS03100 0.17 +0.2 647,940 - AMB_RS03100 0.17 -0.6 647,947 + AMB_RS03100 0.18 -1.0 648,095 + AMB_RS03100 0.42 -1.6 648,095 + AMB_RS03100 0.42 -1.6 648,099 - AMB_RS03100 0.42 +0.2 648,099 - AMB_RS03100 0.42 -0.1 648,099 - AMB_RS03100 0.42 -0.2 648,206 + AMB_RS03100 0.60 +0.3 648,207 - AMB_RS03100 0.60 +0.4 648,207 - AMB_RS03100 0.60 -0.7
Or see this region's nucleotide sequence