Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02570 and AMB_RS02575 are separated by 27 nucleotides AMB_RS02575 and AMB_RS02580 are separated by 12 nucleotides AMB_RS02580 and AMB_RS02585 are separated by 3 nucleotides
AMB_RS02570: AMB_RS02570 - sigma-54-dependent Fis family transcriptional regulator, at 533,683 to 535,056
_RS02570
AMB_RS02575: AMB_RS02575 - flagellar motor switch protein FliN, at 535,084 to 535,410
_RS02575
AMB_RS02580: AMB_RS02580 - flagellar assembly protein H, at 535,423 to 536,100
_RS02580
AMB_RS02585: AMB_RS02585 - flagellar motor switch protein FliG, at 536,104 to 537,123
_RS02585
Position (kb) 535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 534.134 kb on + strand, within AMB_RS02570 at 534.135 kb on - strand, within AMB_RS02570 at 534.135 kb on - strand, within AMB_RS02570 at 534.228 kb on - strand, within AMB_RS02570 at 534.228 kb on - strand, within AMB_RS02570 at 534.228 kb on - strand, within AMB_RS02570 at 534.228 kb on - strand, within AMB_RS02570 at 534.228 kb on - strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.773 kb on + strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 534.774 kb on - strand, within AMB_RS02570 at 535.181 kb on + strand, within AMB_RS02575 at 535.181 kb on + strand, within AMB_RS02575 at 535.181 kb on + strand, within AMB_RS02575 at 535.181 kb on + strand, within AMB_RS02575 at 535.181 kb on + strand, within AMB_RS02575 at 535.307 kb on + strand, within AMB_RS02575 at 535.307 kb on + strand, within AMB_RS02575 at 535.308 kb on - strand, within AMB_RS02575 at 535.424 kb on + strand at 535.665 kb on + strand, within AMB_RS02580 at 535.666 kb on - strand, within AMB_RS02580 at 535.690 kb on + strand, within AMB_RS02580 at 535.690 kb on + strand, within AMB_RS02580 at 535.690 kb on + strand, within AMB_RS02580 at 535.690 kb on + strand, within AMB_RS02580 at 535.690 kb on + strand, within AMB_RS02580 at 535.931 kb on + strand, within AMB_RS02580 at 535.931 kb on + strand, within AMB_RS02580 at 535.932 kb on - strand, within AMB_RS02580 at 536.084 kb on + strand at 536.084 kb on + strand at 536.085 kb on - strand at 536.085 kb on - strand at 536.085 kb on - strand at 536.085 kb on - strand at 536.093 kb on + strand at 536.094 kb on - strand at 536.105 kb on + strand at 536.105 kb on + strand at 536.106 kb on - strand at 536.108 kb on + strand at 536.108 kb on + strand at 536.108 kb on + strand at 536.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 534,134 + AMB_RS02570 0.33 -0.9 534,135 - AMB_RS02570 0.33 -0.1 534,135 - AMB_RS02570 0.33 -2.9 534,228 - AMB_RS02570 0.40 -0.7 534,228 - AMB_RS02570 0.40 -1.3 534,228 - AMB_RS02570 0.40 -1.3 534,228 - AMB_RS02570 0.40 -0.1 534,228 - AMB_RS02570 0.40 +0.1 534,773 + AMB_RS02570 0.79 -1.1 534,773 + AMB_RS02570 0.79 +0.4 534,773 + AMB_RS02570 0.79 -1.1 534,773 + AMB_RS02570 0.79 -0.1 534,773 + AMB_RS02570 0.79 -1.3 534,773 + AMB_RS02570 0.79 +0.1 534,774 - AMB_RS02570 0.79 -0.7 534,774 - AMB_RS02570 0.79 +1.3 534,774 - AMB_RS02570 0.79 -0.3 534,774 - AMB_RS02570 0.79 +0.7 534,774 - AMB_RS02570 0.79 -0.7 534,774 - AMB_RS02570 0.79 -0.1 534,774 - AMB_RS02570 0.79 -1.1 534,774 - AMB_RS02570 0.79 -1.5 534,774 - AMB_RS02570 0.79 -1.3 535,181 + AMB_RS02575 0.30 -0.3 535,181 + AMB_RS02575 0.30 -2.3 535,181 + AMB_RS02575 0.30 -1.0 535,181 + AMB_RS02575 0.30 +0.1 535,181 + AMB_RS02575 0.30 +0.1 535,307 + AMB_RS02575 0.68 +0.3 535,307 + AMB_RS02575 0.68 -0.1 535,308 - AMB_RS02575 0.69 +0.1 535,424 + -0.8 535,665 + AMB_RS02580 0.36 +0.7 535,666 - AMB_RS02580 0.36 +0.0 535,690 + AMB_RS02580 0.39 -0.3 535,690 + AMB_RS02580 0.39 +0.5 535,690 + AMB_RS02580 0.39 +0.3 535,690 + AMB_RS02580 0.39 +0.7 535,690 + AMB_RS02580 0.39 +0.1 535,931 + AMB_RS02580 0.75 -1.1 535,931 + AMB_RS02580 0.75 +0.0 535,932 - AMB_RS02580 0.75 -1.7 536,084 + +0.3 536,084 + +0.4 536,085 - -1.5 536,085 - -1.3 536,085 - -2.6 536,085 - +0.1 536,093 + +1.2 536,094 - +0.1 536,105 + +0.2 536,105 + -1.4 536,106 - -0.0 536,108 + -2.2 536,108 + +0.3 536,108 + +0.5 536,109 - -0.5
Or see this region's nucleotide sequence