Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02270 and AMB_RS02275 are separated by 15 nucleotides AMB_RS02275 and AMB_RS02280 are separated by 3 nucleotides AMB_RS02280 and AMB_RS23120 are separated by 440 nucleotides
AMB_RS02270: AMB_RS02270 - hypothetical protein, at 484,085 to 484,429
_RS02270
AMB_RS02275: AMB_RS02275 - hypothetical protein, at 484,445 to 485,080
_RS02275
AMB_RS02280: AMB_RS02280 - hybrid sensor histidine kinase/response regulator, at 485,084 to 487,267
_RS02280
AMB_RS23120: AMB_RS23120 - helix-turn-helix domain-containing protein, at 487,708 to 488,748
_RS23120
Position (kb) 485
486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 484.326 kb on + strand, within AMB_RS02270 at 484.326 kb on + strand, within AMB_RS02270 at 484.327 kb on - strand, within AMB_RS02270 at 484.327 kb on - strand, within AMB_RS02270 at 484.446 kb on + strand at 484.446 kb on + strand at 484.446 kb on + strand at 484.446 kb on + strand at 484.447 kb on - strand at 484.447 kb on - strand at 484.447 kb on - strand at 484.447 kb on - strand at 484.447 kb on - strand at 484.447 kb on - strand at 484.527 kb on + strand, within AMB_RS02275 at 484.528 kb on - strand, within AMB_RS02275 at 484.599 kb on + strand, within AMB_RS02275 at 484.600 kb on - strand, within AMB_RS02275 at 484.600 kb on - strand, within AMB_RS02275 at 484.600 kb on - strand, within AMB_RS02275 at 484.755 kb on + strand, within AMB_RS02275 at 484.756 kb on - strand, within AMB_RS02275 at 484.756 kb on - strand, within AMB_RS02275 at 484.756 kb on - strand, within AMB_RS02275 at 484.756 kb on - strand, within AMB_RS02275 at 484.791 kb on + strand, within AMB_RS02275 at 484.791 kb on + strand, within AMB_RS02275 at 484.791 kb on + strand, within AMB_RS02275 at 484.791 kb on + strand, within AMB_RS02275 at 484.792 kb on - strand, within AMB_RS02275 at 484.792 kb on - strand, within AMB_RS02275 at 484.792 kb on - strand, within AMB_RS02275 at 484.792 kb on - strand, within AMB_RS02275 at 484.792 kb on - strand, within AMB_RS02275 at 484.872 kb on + strand, within AMB_RS02275 at 484.873 kb on - strand, within AMB_RS02275 at 484.873 kb on - strand, within AMB_RS02275 at 484.873 kb on - strand, within AMB_RS02275 at 484.895 kb on + strand, within AMB_RS02275 at 486.378 kb on + strand, within AMB_RS02280 at 486.378 kb on + strand, within AMB_RS02280 at 486.379 kb on - strand, within AMB_RS02280 at 486.827 kb on + strand, within AMB_RS02280 at 486.827 kb on + strand, within AMB_RS02280 at 486.827 kb on + strand, within AMB_RS02280 at 486.827 kb on + strand, within AMB_RS02280 at 486.828 kb on - strand, within AMB_RS02280 at 486.828 kb on - strand, within AMB_RS02280 at 486.828 kb on - strand, within AMB_RS02280 at 486.828 kb on - strand, within AMB_RS02280 at 487.372 kb on - strand at 487.411 kb on + strand at 487.411 kb on + strand at 487.412 kb on - strand at 487.412 kb on - strand at 487.428 kb on - strand at 487.428 kb on - strand at 487.428 kb on - strand at 487.428 kb on - strand at 487.433 kb on + strand at 487.566 kb on - strand at 487.587 kb on + strand at 487.587 kb on + strand at 487.587 kb on + strand at 487.587 kb on + strand at 487.588 kb on - strand at 487.588 kb on - strand at 487.649 kb on - strand at 487.737 kb on + strand at 487.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 484,326 + AMB_RS02270 0.70 +1.4 484,326 + AMB_RS02270 0.70 -0.3 484,327 - AMB_RS02270 0.70 -0.2 484,327 - AMB_RS02270 0.70 +0.1 484,446 + -1.7 484,446 + -1.8 484,446 + -1.1 484,446 + +0.7 484,447 - +0.2 484,447 - +0.7 484,447 - +0.1 484,447 - -3.1 484,447 - +0.9 484,447 - +0.4 484,527 + AMB_RS02275 0.13 -1.5 484,528 - AMB_RS02275 0.13 +1.1 484,599 + AMB_RS02275 0.24 -0.3 484,600 - AMB_RS02275 0.24 -2.3 484,600 - AMB_RS02275 0.24 +0.6 484,600 - AMB_RS02275 0.24 -0.1 484,755 + AMB_RS02275 0.49 -0.9 484,756 - AMB_RS02275 0.49 -2.1 484,756 - AMB_RS02275 0.49 +0.8 484,756 - AMB_RS02275 0.49 +1.6 484,756 - AMB_RS02275 0.49 -0.7 484,791 + AMB_RS02275 0.54 -0.7 484,791 + AMB_RS02275 0.54 -1.3 484,791 + AMB_RS02275 0.54 +0.2 484,791 + AMB_RS02275 0.54 -0.5 484,792 - AMB_RS02275 0.55 -0.1 484,792 - AMB_RS02275 0.55 +0.3 484,792 - AMB_RS02275 0.55 -1.1 484,792 - AMB_RS02275 0.55 -1.2 484,792 - AMB_RS02275 0.55 -0.1 484,872 + AMB_RS02275 0.67 -1.1 484,873 - AMB_RS02275 0.67 +1.5 484,873 - AMB_RS02275 0.67 +0.3 484,873 - AMB_RS02275 0.67 -2.7 484,895 + AMB_RS02275 0.71 -0.1 486,378 + AMB_RS02280 0.59 +0.6 486,378 + AMB_RS02280 0.59 -1.8 486,379 - AMB_RS02280 0.59 -0.8 486,827 + AMB_RS02280 0.80 -1.8 486,827 + AMB_RS02280 0.80 +0.7 486,827 + AMB_RS02280 0.80 +0.3 486,827 + AMB_RS02280 0.80 -0.5 486,828 - AMB_RS02280 0.80 -0.2 486,828 - AMB_RS02280 0.80 -0.1 486,828 - AMB_RS02280 0.80 -0.9 486,828 - AMB_RS02280 0.80 +0.1 487,372 - -0.7 487,411 + -0.7 487,411 + -0.9 487,412 - -1.5 487,412 - -0.9 487,428 - -1.8 487,428 - -1.9 487,428 - -0.0 487,428 - -0.8 487,433 + -1.1 487,566 - -0.8 487,587 + -0.5 487,587 + -1.5 487,587 + -0.1 487,587 + -0.2 487,588 - -2.4 487,588 - -2.4 487,649 - -2.0 487,737 + +0.1 487,738 - -1.1
Or see this region's nucleotide sequence