Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23845 and AMB_RS02050 are separated by 60 nucleotides AMB_RS02050 and AMB_RS02055 overlap by 4 nucleotides AMB_RS02055 and AMB_RS02060 are separated by 95 nucleotides
AMB_RS23845: AMB_RS23845 - hypothetical protein, at 433,821 to 433,973
_RS23845
AMB_RS02050: AMB_RS02050 - transposase, at 434,034 to 434,384
_RS02050
AMB_RS02055: AMB_RS02055 - IS66 family insertion sequence hypothetical protein, at 434,381 to 434,728
_RS02055
AMB_RS02060: AMB_RS02060 - transposase, at 434,824 to 436,361
_RS02060
Position (kb) 434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 433.389 kb on + strand at 433.389 kb on + strand at 433.390 kb on - strand at 433.390 kb on - strand at 433.390 kb on - strand at 433.390 kb on - strand at 433.393 kb on + strand at 433.393 kb on + strand at 433.393 kb on + strand at 433.394 kb on - strand at 433.394 kb on - strand at 433.394 kb on - strand at 433.394 kb on - strand at 433.723 kb on - strand at 433.723 kb on - strand at 433.723 kb on - strand at 433.756 kb on + strand at 433.757 kb on - strand at 433.827 kb on + strand at 433.827 kb on + strand at 433.898 kb on + strand, within AMB_RS23845 at 433.898 kb on + strand, within AMB_RS23845 at 433.898 kb on + strand, within AMB_RS23845 at 433.899 kb on - strand, within AMB_RS23845 at 433.899 kb on - strand, within AMB_RS23845 at 433.933 kb on + strand, within AMB_RS23845 at 433.934 kb on - strand, within AMB_RS23845 at 433.968 kb on + strand at 433.968 kb on + strand at 433.968 kb on + strand at 433.968 kb on + strand at 433.969 kb on - strand at 433.969 kb on - strand at 434.181 kb on + strand, within AMB_RS02050 at 434.230 kb on + strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.231 kb on - strand, within AMB_RS02050 at 434.663 kb on + strand, within AMB_RS02055 at 435.063 kb on + strand, within AMB_RS02060 at 435.063 kb on + strand, within AMB_RS02060 at 435.365 kb on + strand, within AMB_RS02060 at 435.365 kb on + strand, within AMB_RS02060 at 435.365 kb on + strand, within AMB_RS02060 at 435.366 kb on - strand, within AMB_RS02060 at 435.366 kb on - strand, within AMB_RS02060 at 435.612 kb on - strand, within AMB_RS02060 at 435.612 kb on - strand, within AMB_RS02060 at 435.612 kb on - strand, within AMB_RS02060 at 435.670 kb on + strand, within AMB_RS02060 at 435.670 kb on + strand, within AMB_RS02060 at 435.671 kb on - strand, within AMB_RS02060 at 435.671 kb on - strand, within AMB_RS02060 at 435.671 kb on - strand, within AMB_RS02060 at 435.671 kb on - strand, within AMB_RS02060 at 435.671 kb on - strand, within AMB_RS02060
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 433,389 + -2.2 433,389 + -0.8 433,390 - +0.3 433,390 - -0.3 433,390 - +0.3 433,390 - +0.1 433,393 + -0.1 433,393 + +0.7 433,393 + -0.3 433,394 - -0.1 433,394 - +0.1 433,394 - -0.3 433,394 - -0.3 433,723 - -2.6 433,723 - -0.3 433,723 - +0.7 433,756 + -1.2 433,757 - -0.3 433,827 + -2.1 433,827 + +0.7 433,898 + AMB_RS23845 0.50 +0.7 433,898 + AMB_RS23845 0.50 +0.3 433,898 + AMB_RS23845 0.50 -0.6 433,899 - AMB_RS23845 0.51 -0.1 433,899 - AMB_RS23845 0.51 -0.1 433,933 + AMB_RS23845 0.73 +0.7 433,934 - AMB_RS23845 0.74 -1.8 433,968 + -0.0 433,968 + -0.6 433,968 + -1.6 433,968 + -0.6 433,969 - +0.0 433,969 - +0.8 434,181 + AMB_RS02050 0.42 +0.8 434,230 + AMB_RS02050 0.56 +1.7 434,231 - AMB_RS02050 0.56 +0.1 434,231 - AMB_RS02050 0.56 +0.1 434,231 - AMB_RS02050 0.56 -0.1 434,231 - AMB_RS02050 0.56 -1.0 434,231 - AMB_RS02050 0.56 +0.2 434,231 - AMB_RS02050 0.56 +0.2 434,231 - AMB_RS02050 0.56 +0.7 434,663 + AMB_RS02055 0.81 +1.7 435,063 + AMB_RS02060 0.16 -1.1 435,063 + AMB_RS02060 0.16 +0.3 435,365 + AMB_RS02060 0.35 -0.4 435,365 + AMB_RS02060 0.35 -0.1 435,365 + AMB_RS02060 0.35 -0.1 435,366 - AMB_RS02060 0.35 -0.9 435,366 - AMB_RS02060 0.35 +0.7 435,612 - AMB_RS02060 0.51 -0.7 435,612 - AMB_RS02060 0.51 +1.5 435,612 - AMB_RS02060 0.51 -0.1 435,670 + AMB_RS02060 0.55 +0.1 435,670 + AMB_RS02060 0.55 -2.9 435,671 - AMB_RS02060 0.55 -1.3 435,671 - AMB_RS02060 0.55 +0.3 435,671 - AMB_RS02060 0.55 -0.1 435,671 - AMB_RS02060 0.55 -0.3 435,671 - AMB_RS02060 0.55 +0.1
Or see this region's nucleotide sequence