Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01960 and AMB_RS01965 are separated by 6 nucleotides AMB_RS01965 and AMB_RS01970 are separated by 10 nucleotides AMB_RS01970 and AMB_RS01975 are separated by 4 nucleotides AMB_RS01975 and AMB_RS01980 are separated by 2 nucleotides
AMB_RS01960: AMB_RS01960 - hypothetical protein, at 415,699 to 416,196
_RS01960
AMB_RS01965: AMB_RS01965 - hypothetical protein, at 416,203 to 417,072
_RS01965
AMB_RS01970: AMB_RS01970 - hypothetical protein, at 417,083 to 417,493
_RS01970
AMB_RS01975: AMB_RS01975 - hypothetical protein, at 417,498 to 418,064
_RS01975
AMB_RS01980: AMB_RS01980 - hypothetical protein, at 418,067 to 419,410
_RS01980
Position (kb)
417
418 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 416.155 kb on - strand at 416.155 kb on - strand at 416.155 kb on - strand at 416.155 kb on - strand at 416.350 kb on - strand, within AMB_RS01965 at 416.478 kb on + strand, within AMB_RS01965 at 416.479 kb on - strand, within AMB_RS01965 at 417.043 kb on + strand at 417.044 kb on - strand at 417.101 kb on + strand at 417.243 kb on - strand, within AMB_RS01970 at 417.243 kb on - strand, within AMB_RS01970 at 417.288 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.792 kb on + strand, within AMB_RS01975 at 417.792 kb on + strand, within AMB_RS01975 at 417.792 kb on + strand, within AMB_RS01975 at 417.876 kb on + strand, within AMB_RS01975 at 417.876 kb on + strand, within AMB_RS01975 at 417.902 kb on + strand, within AMB_RS01975 at 417.903 kb on - strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.012 kb on - strand at 418.023 kb on + strand at 418.023 kb on + strand at 418.023 kb on + strand at 418.025 kb on + strand at 418.052 kb on + strand at 418.053 kb on - strand at 418.100 kb on + strand at 418.111 kb on + strand at 418.111 kb on + strand at 418.200 kb on - strand at 418.200 kb on - strand at 418.209 kb on - strand, within AMB_RS01980 at 418.209 kb on - strand, within AMB_RS01980 at 418.235 kb on + strand, within AMB_RS01980 at 418.235 kb on + strand, within AMB_RS01980 at 418.235 kb on + strand, within AMB_RS01980 at 418.235 kb on + strand, within AMB_RS01980 at 418.235 kb on + strand, within AMB_RS01980 at 418.236 kb on - strand, within AMB_RS01980 at 418.236 kb on - strand, within AMB_RS01980 at 418.236 kb on - strand, within AMB_RS01980 at 418.250 kb on + strand, within AMB_RS01980 at 418.250 kb on + strand, within AMB_RS01980 at 418.252 kb on + strand, within AMB_RS01980 at 418.252 kb on + strand, within AMB_RS01980 at 418.252 kb on + strand, within AMB_RS01980 at 418.252 kb on + strand, within AMB_RS01980 at 418.252 kb on + strand, within AMB_RS01980 at 418.301 kb on + strand, within AMB_RS01980 at 418.301 kb on + strand, within AMB_RS01980 at 418.301 kb on + strand, within AMB_RS01980 at 418.302 kb on - strand, within AMB_RS01980 at 418.302 kb on - strand, within AMB_RS01980 at 418.307 kb on + strand, within AMB_RS01980 at 418.349 kb on + strand, within AMB_RS01980 at 418.349 kb on + strand, within AMB_RS01980 at 418.349 kb on + strand, within AMB_RS01980 at 418.350 kb on - strand, within AMB_RS01980 at 418.350 kb on - strand, within AMB_RS01980 at 418.387 kb on + strand, within AMB_RS01980 at 418.440 kb on - strand, within AMB_RS01980 at 418.440 kb on - strand, within AMB_RS01980
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 416,155 - +0.7 416,155 - -0.0 416,155 - -1.3 416,155 - -0.3 416,350 - AMB_RS01965 0.17 -1.8 416,478 + AMB_RS01965 0.32 -1.0 416,479 - AMB_RS01965 0.32 -0.6 417,043 + -0.6 417,044 - +0.7 417,101 + +0.7 417,243 - AMB_RS01970 0.39 +0.7 417,243 - AMB_RS01970 0.39 +0.1 417,288 - AMB_RS01970 0.50 -0.3 417,301 - AMB_RS01970 0.53 -1.1 417,301 - AMB_RS01970 0.53 -0.3 417,301 - AMB_RS01970 0.53 +0.3 417,301 - AMB_RS01970 0.53 +0.1 417,480 - +1.2 417,480 - -3.2 417,480 - +0.1 417,480 - +0.7 417,480 - +0.7 417,480 - -0.2 417,792 + AMB_RS01975 0.52 +2.0 417,792 + AMB_RS01975 0.52 +0.3 417,792 + AMB_RS01975 0.52 +0.7 417,876 + AMB_RS01975 0.67 -0.6 417,876 + AMB_RS01975 0.67 +0.0 417,902 + AMB_RS01975 0.71 +0.6 417,903 - AMB_RS01975 0.71 +0.2 417,939 + AMB_RS01975 0.78 -0.1 417,939 + AMB_RS01975 0.78 +0.2 417,939 + AMB_RS01975 0.78 -0.5 417,939 + AMB_RS01975 0.78 -0.6 417,940 - AMB_RS01975 0.78 +0.5 417,940 - AMB_RS01975 0.78 -0.6 417,940 - AMB_RS01975 0.78 -0.0 417,999 + AMB_RS01975 0.88 +0.1 417,999 + AMB_RS01975 0.88 -0.9 417,999 + AMB_RS01975 0.88 +0.4 417,999 + AMB_RS01975 0.88 +0.1 417,999 + AMB_RS01975 0.88 +0.1 418,000 - AMB_RS01975 0.89 -0.1 418,000 - AMB_RS01975 0.89 -0.6 418,000 - AMB_RS01975 0.89 +0.8 418,000 - AMB_RS01975 0.89 -0.4 418,012 - -0.3 418,023 + +0.4 418,023 + -1.1 418,023 + -2.6 418,025 + -0.1 418,052 + +0.3 418,053 - +0.2 418,100 + -0.4 418,111 + +0.1 418,111 + -0.0 418,200 - -0.3 418,200 - -0.8 418,209 - AMB_RS01980 0.11 +0.6 418,209 - AMB_RS01980 0.11 +0.8 418,235 + AMB_RS01980 0.12 +0.8 418,235 + AMB_RS01980 0.12 -1.1 418,235 + AMB_RS01980 0.12 +0.0 418,235 + AMB_RS01980 0.12 -0.3 418,235 + AMB_RS01980 0.12 +0.4 418,236 - AMB_RS01980 0.13 -0.4 418,236 - AMB_RS01980 0.13 -1.5 418,236 - AMB_RS01980 0.13 -0.0 418,250 + AMB_RS01980 0.14 -0.5 418,250 + AMB_RS01980 0.14 -0.0 418,252 + AMB_RS01980 0.14 -2.8 418,252 + AMB_RS01980 0.14 -0.5 418,252 + AMB_RS01980 0.14 +0.7 418,252 + AMB_RS01980 0.14 -0.7 418,252 + AMB_RS01980 0.14 -0.3 418,301 + AMB_RS01980 0.17 +1.7 418,301 + AMB_RS01980 0.17 +0.5 418,301 + AMB_RS01980 0.17 +0.7 418,302 - AMB_RS01980 0.17 -0.9 418,302 - AMB_RS01980 0.17 -1.3 418,307 + AMB_RS01980 0.18 +0.6 418,349 + AMB_RS01980 0.21 -2.2 418,349 + AMB_RS01980 0.21 -0.1 418,349 + AMB_RS01980 0.21 +0.3 418,350 - AMB_RS01980 0.21 +0.1 418,350 - AMB_RS01980 0.21 +1.4 418,387 + AMB_RS01980 0.24 -0.1 418,440 - AMB_RS01980 0.28 -2.1 418,440 - AMB_RS01980 0.28 -0.1
Or see this region's nucleotide sequence