Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01790 and AMB_RS01795 are separated by 16 nucleotides AMB_RS01795 and AMB_RS01800 are separated by 85 nucleotides AMB_RS01800 and AMB_RS01805 overlap by 4 nucleotides AMB_RS01805 and AMB_RS01810 overlap by 7 nucleotides
AMB_RS01790: AMB_RS01790 - oxidoreductase, at 387,348 to 388,103
_RS01790
AMB_RS01795: AMB_RS01795 - DUF669 domain-containing protein, at 388,120 to 388,626
_RS01795
AMB_RS01800: AMB_RS01800 - hypothetical protein, at 388,712 to 389,089
_RS01800
AMB_RS01805: AMB_RS01805 - hypothetical protein, at 389,086 to 390,015
_RS01805
AMB_RS01810: AMB_RS01810 - DNA helicase, at 390,009 to 391,580
_RS01810
Position (kb) 389
390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 388.102 kb on - strand at 388.195 kb on + strand, within AMB_RS01795 at 388.195 kb on + strand, within AMB_RS01795 at 388.195 kb on + strand, within AMB_RS01795 at 388.196 kb on - strand, within AMB_RS01795 at 388.196 kb on - strand, within AMB_RS01795 at 388.196 kb on - strand, within AMB_RS01795 at 388.196 kb on - strand, within AMB_RS01795 at 388.252 kb on + strand, within AMB_RS01795 at 388.253 kb on - strand, within AMB_RS01795 at 388.253 kb on - strand, within AMB_RS01795 at 388.253 kb on - strand, within AMB_RS01795 at 388.728 kb on + strand at 388.728 kb on + strand at 388.728 kb on + strand at 388.728 kb on + strand at 388.728 kb on + strand at 388.728 kb on + strand at 388.728 kb on + strand at 388.729 kb on - strand at 388.729 kb on - strand at 388.729 kb on - strand at 388.729 kb on - strand at 388.843 kb on + strand, within AMB_RS01800 at 388.843 kb on + strand, within AMB_RS01800 at 388.843 kb on + strand, within AMB_RS01800 at 388.844 kb on - strand, within AMB_RS01800 at 388.844 kb on - strand, within AMB_RS01800 at 388.844 kb on - strand, within AMB_RS01800 at 388.887 kb on - strand, within AMB_RS01800 at 389.015 kb on + strand, within AMB_RS01800 at 389.016 kb on - strand, within AMB_RS01800 at 389.085 kb on + strand at 389.085 kb on + strand at 389.086 kb on - strand at 389.086 kb on - strand at 389.086 kb on - strand at 389.110 kb on - strand at 389.131 kb on + strand at 389.132 kb on - strand at 389.132 kb on - strand at 389.132 kb on - strand at 389.140 kb on + strand at 389.140 kb on + strand at 389.140 kb on + strand at 389.140 kb on + strand at 389.141 kb on - strand at 389.141 kb on - strand at 389.141 kb on - strand at 389.433 kb on - strand, within AMB_RS01805 at 389.433 kb on - strand, within AMB_RS01805 at 389.557 kb on + strand, within AMB_RS01805 at 389.557 kb on + strand, within AMB_RS01805 at 389.558 kb on - strand, within AMB_RS01805 at 389.558 kb on - strand, within AMB_RS01805 at 389.590 kb on + strand, within AMB_RS01805 at 389.590 kb on + strand, within AMB_RS01805 at 389.590 kb on + strand, within AMB_RS01805 at 389.590 kb on + strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.591 kb on - strand, within AMB_RS01805 at 389.624 kb on - strand, within AMB_RS01805 at 389.624 kb on - strand, within AMB_RS01805 at 389.908 kb on + strand, within AMB_RS01805 at 389.908 kb on + strand, within AMB_RS01805 at 389.909 kb on - strand, within AMB_RS01805 at 389.909 kb on - strand, within AMB_RS01805 at 389.909 kb on - strand, within AMB_RS01805 at 389.909 kb on - strand, within AMB_RS01805 at 389.909 kb on - strand, within AMB_RS01805 at 390.055 kb on - strand at 390.055 kb on - strand at 390.061 kb on - strand at 390.216 kb on + strand, within AMB_RS01810 at 390.216 kb on + strand, within AMB_RS01810 at 390.217 kb on - strand, within AMB_RS01810 at 390.217 kb on - strand, within AMB_RS01810 at 390.217 kb on - strand, within AMB_RS01810 at 390.217 kb on - strand, within AMB_RS01810 at 390.261 kb on + strand, within AMB_RS01810 at 390.261 kb on + strand, within AMB_RS01810 at 390.262 kb on - strand, within AMB_RS01810 at 390.262 kb on - strand, within AMB_RS01810 at 390.262 kb on - strand, within AMB_RS01810 at 390.339 kb on + strand, within AMB_RS01810 at 390.339 kb on + strand, within AMB_RS01810 at 390.339 kb on + strand, within AMB_RS01810 at 390.340 kb on - strand, within AMB_RS01810 at 390.340 kb on - strand, within AMB_RS01810 at 390.340 kb on - strand, within AMB_RS01810 at 390.340 kb on - strand, within AMB_RS01810 at 390.340 kb on - strand, within AMB_RS01810 at 390.412 kb on - strand, within AMB_RS01810 at 390.470 kb on + strand, within AMB_RS01810 at 390.730 kb on - strand, within AMB_RS01810 at 390.830 kb on + strand, within AMB_RS01810 at 390.831 kb on - strand, within AMB_RS01810 at 390.831 kb on - strand, within AMB_RS01810 at 390.993 kb on + strand, within AMB_RS01810 at 390.993 kb on + strand, within AMB_RS01810 at 390.993 kb on + strand, within AMB_RS01810 at 390.993 kb on + strand, within AMB_RS01810 at 390.994 kb on - strand, within AMB_RS01810 at 390.994 kb on - strand, within AMB_RS01810 at 390.994 kb on - strand, within AMB_RS01810
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 388,102 - +0.7 388,195 + AMB_RS01795 0.15 -0.9 388,195 + AMB_RS01795 0.15 +0.7 388,195 + AMB_RS01795 0.15 -1.2 388,196 - AMB_RS01795 0.15 -1.5 388,196 - AMB_RS01795 0.15 -0.7 388,196 - AMB_RS01795 0.15 +0.0 388,196 - AMB_RS01795 0.15 -1.1 388,252 + AMB_RS01795 0.26 -3.4 388,253 - AMB_RS01795 0.26 -0.5 388,253 - AMB_RS01795 0.26 -0.3 388,253 - AMB_RS01795 0.26 -0.3 388,728 + -0.9 388,728 + -0.7 388,728 + +0.1 388,728 + -1.1 388,728 + -0.3 388,728 + -0.6 388,728 + -0.7 388,729 - -1.3 388,729 - +0.7 388,729 - +0.3 388,729 - -0.1 388,843 + AMB_RS01800 0.35 +0.8 388,843 + AMB_RS01800 0.35 -0.3 388,843 + AMB_RS01800 0.35 +1.2 388,844 - AMB_RS01800 0.35 +0.1 388,844 - AMB_RS01800 0.35 -0.1 388,844 - AMB_RS01800 0.35 +0.2 388,887 - AMB_RS01800 0.46 -2.0 389,015 + AMB_RS01800 0.80 -0.6 389,016 - AMB_RS01800 0.80 -1.9 389,085 + -3.0 389,085 + -0.5 389,086 - -0.9 389,086 - -0.3 389,086 - +0.1 389,110 - -0.5 389,131 + +1.1 389,132 - -0.3 389,132 - -0.1 389,132 - -0.9 389,140 + -1.8 389,140 + -0.7 389,140 + -1.1 389,140 + -1.3 389,141 - -1.1 389,141 - -0.0 389,141 - +0.1 389,433 - AMB_RS01805 0.37 +0.1 389,433 - AMB_RS01805 0.37 +0.7 389,557 + AMB_RS01805 0.51 +0.1 389,557 + AMB_RS01805 0.51 -2.1 389,558 - AMB_RS01805 0.51 -1.3 389,558 - AMB_RS01805 0.51 -0.8 389,590 + AMB_RS01805 0.54 -2.4 389,590 + AMB_RS01805 0.54 -2.1 389,590 + AMB_RS01805 0.54 -0.4 389,590 + AMB_RS01805 0.54 -0.6 389,591 - AMB_RS01805 0.54 -0.1 389,591 - AMB_RS01805 0.54 -0.6 389,591 - AMB_RS01805 0.54 -0.9 389,591 - AMB_RS01805 0.54 -0.7 389,591 - AMB_RS01805 0.54 -0.1 389,591 - AMB_RS01805 0.54 -1.8 389,591 - AMB_RS01805 0.54 -0.1 389,591 - AMB_RS01805 0.54 -2.3 389,591 - AMB_RS01805 0.54 -0.2 389,591 - AMB_RS01805 0.54 -0.4 389,591 - AMB_RS01805 0.54 -0.9 389,624 - AMB_RS01805 0.58 +0.9 389,624 - AMB_RS01805 0.58 -3.3 389,908 + AMB_RS01805 0.88 -1.4 389,908 + AMB_RS01805 0.88 -0.7 389,909 - AMB_RS01805 0.88 -0.6 389,909 - AMB_RS01805 0.88 -0.1 389,909 - AMB_RS01805 0.88 -1.5 389,909 - AMB_RS01805 0.88 -1.3 389,909 - AMB_RS01805 0.88 +0.1 390,055 - -2.7 390,055 - +0.1 390,061 - +0.0 390,216 + AMB_RS01810 0.13 -0.3 390,216 + AMB_RS01810 0.13 -2.1 390,217 - AMB_RS01810 0.13 -0.3 390,217 - AMB_RS01810 0.13 +0.2 390,217 - AMB_RS01810 0.13 +0.1 390,217 - AMB_RS01810 0.13 -0.9 390,261 + AMB_RS01810 0.16 -0.9 390,261 + AMB_RS01810 0.16 +0.4 390,262 - AMB_RS01810 0.16 -0.2 390,262 - AMB_RS01810 0.16 -0.3 390,262 - AMB_RS01810 0.16 +0.0 390,339 + AMB_RS01810 0.21 +0.7 390,339 + AMB_RS01810 0.21 +0.7 390,339 + AMB_RS01810 0.21 +0.1 390,340 - AMB_RS01810 0.21 -1.3 390,340 - AMB_RS01810 0.21 +0.1 390,340 - AMB_RS01810 0.21 +0.7 390,340 - AMB_RS01810 0.21 -0.5 390,340 - AMB_RS01810 0.21 +1.0 390,412 - AMB_RS01810 0.26 -0.1 390,470 + AMB_RS01810 0.29 -1.7 390,730 - AMB_RS01810 0.46 -0.7 390,830 + AMB_RS01810 0.52 -1.3 390,831 - AMB_RS01810 0.52 -2.0 390,831 - AMB_RS01810 0.52 -0.6 390,993 + AMB_RS01810 0.63 -0.7 390,993 + AMB_RS01810 0.63 +0.4 390,993 + AMB_RS01810 0.63 -0.5 390,993 + AMB_RS01810 0.63 -1.8 390,994 - AMB_RS01810 0.63 -0.3 390,994 - AMB_RS01810 0.63 -2.1 390,994 - AMB_RS01810 0.63 -1.1
Or see this region's nucleotide sequence