Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01250 and AMB_RS01255 are separated by 92 nucleotides AMB_RS01255 and AMB_RS01260 overlap by 1 nucleotides AMB_RS01260 and AMB_RS01265 are separated by 111 nucleotides
AMB_RS01250: AMB_RS01250 - hypothetical protein, at 278,895 to 279,236
_RS01250
AMB_RS01255: AMB_RS01255 - 7-carboxy-7-deazaguanine synthase, at 279,329 to 279,937
_RS01255
AMB_RS01260: AMB_RS01260 - 6-carboxytetrahydropterin synthase, at 279,937 to 280,461
_RS01260
AMB_RS01265: AMB_RS01265 - LysR family transcriptional regulator, at 280,573 to 281,460
_RS01265
Position (kb) 279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 279.189 kb on + strand, within AMB_RS01250 at 279.311 kb on + strand at 279.311 kb on + strand at 279.311 kb on + strand at 279.311 kb on + strand at 279.320 kb on - strand at 279.335 kb on + strand at 279.336 kb on - strand at 279.336 kb on - strand at 279.503 kb on + strand, within AMB_RS01255 at 279.504 kb on - strand, within AMB_RS01255 at 279.809 kb on + strand, within AMB_RS01255 at 279.810 kb on - strand, within AMB_RS01255 at 279.810 kb on - strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.935 kb on + strand at 279.935 kb on + strand at 279.935 kb on + strand at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.304 kb on - strand, within AMB_RS01260 at 280.304 kb on - strand, within AMB_RS01260 at 280.447 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.469 kb on - strand at 280.469 kb on - strand at 280.477 kb on - strand at 280.477 kb on - strand at 280.498 kb on + strand at 280.498 kb on + strand at 280.539 kb on + strand at 280.595 kb on - strand at 281.013 kb on + strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 279,189 + AMB_RS01250 0.86 +0.2 279,311 + -1.2 279,311 + +0.9 279,311 + -0.6 279,311 + -0.8 279,320 - -0.6 279,335 + -0.8 279,336 - -1.1 279,336 - +0.4 279,503 + AMB_RS01255 0.29 -0.4 279,504 - AMB_RS01255 0.29 +0.4 279,809 + AMB_RS01255 0.79 +0.8 279,810 - AMB_RS01255 0.79 -1.0 279,810 - AMB_RS01255 0.79 +0.2 279,853 + AMB_RS01255 0.86 +0.1 279,853 + AMB_RS01255 0.86 -2.2 279,853 + AMB_RS01255 0.86 +0.2 279,853 + AMB_RS01255 0.86 +0.1 279,853 + AMB_RS01255 0.86 +1.8 279,854 - AMB_RS01255 0.86 -0.2 279,854 - AMB_RS01255 0.86 -0.6 279,854 - AMB_RS01255 0.86 +0.6 279,854 - AMB_RS01255 0.86 -0.8 279,854 - AMB_RS01255 0.86 -2.5 279,854 - AMB_RS01255 0.86 +1.3 279,935 + +0.8 279,935 + +0.8 279,935 + -1.7 280,033 + AMB_RS01260 0.18 -0.8 280,033 + AMB_RS01260 0.18 +1.0 280,033 + AMB_RS01260 0.18 -0.2 280,033 + AMB_RS01260 0.18 -0.6 280,034 - AMB_RS01260 0.18 +0.1 280,034 - AMB_RS01260 0.18 -1.2 280,034 - AMB_RS01260 0.18 -1.2 280,034 - AMB_RS01260 0.18 -3.5 280,303 + AMB_RS01260 0.70 -0.3 280,303 + AMB_RS01260 0.70 -1.0 280,303 + AMB_RS01260 0.70 -1.2 280,304 - AMB_RS01260 0.70 -0.4 280,304 - AMB_RS01260 0.70 +0.1 280,447 + +0.3 280,468 + -0.0 280,468 + +0.6 280,468 + -0.2 280,468 + +0.1 280,468 + +0.3 280,468 + -1.4 280,468 + +0.1 280,469 - -0.2 280,469 - +0.2 280,477 - +0.8 280,477 - +0.3 280,498 + -1.9 280,498 + +0.0 280,539 + +0.2 280,595 - -0.8 281,013 + AMB_RS01265 0.50 -0.6 281,014 - AMB_RS01265 0.50 -1.8 281,014 - AMB_RS01265 0.50 -0.2 281,014 - AMB_RS01265 0.50 -1.4
Or see this region's nucleotide sequence