Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01105 and AMB_RS01110 are separated by 0 nucleotides AMB_RS01110 and AMB_RS23825 are separated by 127 nucleotides AMB_RS23825 and AMB_RS01115 are separated by 44 nucleotides AMB_RS01115 and AMB_RS01120 are separated by 61 nucleotides
AMB_RS01105: AMB_RS01105 - rhodanese-like domain-containing protein, at 246,639 to 247,049
_RS01105
AMB_RS01110: AMB_RS01110 - hypothetical protein, at 247,050 to 247,445
_RS01110
AMB_RS23825: AMB_RS23825 - acylphosphatase, at 247,573 to 247,856
_RS23825
AMB_RS01115: AMB_RS01115 - beta-glucosidase, at 247,901 to 249,262
_RS01115
AMB_RS01120: AMB_RS01120 - HAMP domain-containing protein, at 249,324 to 251,747
_RS01120
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 247.087 kb on + strand at 247.087 kb on + strand at 247.088 kb on - strand at 247.088 kb on - strand at 247.088 kb on - strand at 247.088 kb on - strand at 247.213 kb on + strand, within AMB_RS01110 at 247.214 kb on - strand, within AMB_RS01110 at 247.214 kb on - strand, within AMB_RS01110 at 247.214 kb on - strand, within AMB_RS01110 at 247.214 kb on - strand, within AMB_RS01110 at 247.216 kb on - strand, within AMB_RS01110 at 247.219 kb on + strand, within AMB_RS01110 at 247.548 kb on + strand at 247.548 kb on + strand at 247.548 kb on + strand at 247.548 kb on + strand at 247.548 kb on + strand at 247.549 kb on - strand at 247.549 kb on - strand at 247.549 kb on - strand at 247.549 kb on - strand at 247.550 kb on + strand at 247.550 kb on + strand at 247.551 kb on - strand at 247.551 kb on - strand at 247.552 kb on + strand at 247.552 kb on + strand at 247.553 kb on - strand at 248.006 kb on + strand at 248.007 kb on - strand at 248.007 kb on - strand at 248.103 kb on - strand, within AMB_RS01115 at 248.103 kb on - strand, within AMB_RS01115 at 248.231 kb on + strand, within AMB_RS01115 at 248.231 kb on + strand, within AMB_RS01115 at 248.232 kb on - strand, within AMB_RS01115 at 248.288 kb on + strand, within AMB_RS01115 at 248.288 kb on + strand, within AMB_RS01115 at 248.414 kb on + strand, within AMB_RS01115 at 248.414 kb on + strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.740 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.802 kb on - strand, within AMB_RS01115 at 248.804 kb on + strand, within AMB_RS01115 at 248.805 kb on - strand, within AMB_RS01115 at 248.918 kb on + strand, within AMB_RS01115 at 248.919 kb on - strand, within AMB_RS01115 at 248.990 kb on + strand, within AMB_RS01115 at 248.991 kb on - strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.047 kb on - strand, within AMB_RS01115 at 249.146 kb on + strand at 249.146 kb on + strand at 249.146 kb on + strand at 249.147 kb on - strand at 249.196 kb on - strand at 249.196 kb on - strand at 249.202 kb on + strand at 249.203 kb on - strand at 249.203 kb on - strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.306 kb on + strand at 249.306 kb on + strand at 249.306 kb on + strand at 249.383 kb on + strand at 249.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 247,087 + +0.9 247,087 + -2.3 247,088 - +0.3 247,088 - +1.0 247,088 - +0.2 247,088 - +0.8 247,213 + AMB_RS01110 0.41 -0.1 247,214 - AMB_RS01110 0.41 +0.1 247,214 - AMB_RS01110 0.41 -2.1 247,214 - AMB_RS01110 0.41 +1.0 247,214 - AMB_RS01110 0.41 +0.2 247,216 - AMB_RS01110 0.42 +0.2 247,219 + AMB_RS01110 0.43 -0.6 247,548 + +0.1 247,548 + +0.1 247,548 + -1.2 247,548 + +0.2 247,548 + -0.1 247,549 - -0.8 247,549 - +0.4 247,549 - -0.7 247,549 - +0.2 247,550 + -0.2 247,550 + -2.9 247,551 - -2.4 247,551 - +0.8 247,552 + -0.0 247,552 + +0.1 247,553 - +0.3 248,006 + -0.3 248,007 - +1.8 248,007 - -1.9 248,103 - AMB_RS01115 0.15 -0.1 248,103 - AMB_RS01115 0.15 +0.6 248,231 + AMB_RS01115 0.24 -0.5 248,231 + AMB_RS01115 0.24 +0.6 248,232 - AMB_RS01115 0.24 -0.8 248,288 + AMB_RS01115 0.28 -2.2 248,288 + AMB_RS01115 0.28 -1.2 248,414 + AMB_RS01115 0.38 +1.8 248,414 + AMB_RS01115 0.38 -0.2 248,415 - AMB_RS01115 0.38 +0.5 248,415 - AMB_RS01115 0.38 -1.0 248,415 - AMB_RS01115 0.38 +0.2 248,415 - AMB_RS01115 0.38 +0.2 248,740 + AMB_RS01115 0.62 -1.2 248,801 + AMB_RS01115 0.66 +0.8 248,801 + AMB_RS01115 0.66 -0.6 248,801 + AMB_RS01115 0.66 -1.8 248,802 - AMB_RS01115 0.66 -0.6 248,804 + AMB_RS01115 0.66 -0.9 248,805 - AMB_RS01115 0.66 +0.1 248,918 + AMB_RS01115 0.75 -1.9 248,919 - AMB_RS01115 0.75 -0.1 248,990 + AMB_RS01115 0.80 -0.7 248,991 - AMB_RS01115 0.80 -0.6 249,046 + AMB_RS01115 0.84 -0.7 249,046 + AMB_RS01115 0.84 +0.8 249,046 + AMB_RS01115 0.84 -0.2 249,047 - AMB_RS01115 0.84 -0.5 249,146 + -0.3 249,146 + -0.8 249,146 + -0.8 249,147 - -0.3 249,196 - -0.4 249,196 - -0.0 249,202 + -0.3 249,203 - +0.0 249,203 - -0.4 249,218 + +0.8 249,218 + -0.3 249,218 + +0.8 249,218 + -0.7 249,219 - -1.0 249,219 - +0.8 249,219 - -2.1 249,219 - -0.4 249,219 - +1.8 249,219 - -0.7 249,219 - -0.5 249,219 - +0.1 249,306 + -0.3 249,306 + -1.0 249,306 + +0.0 249,383 + -0.4 249,523 + +1.8
Or see this region's nucleotide sequence