Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00110 and AMB_RS00115 are separated by 24 nucleotides AMB_RS00115 and AMB_RS00120 are separated by 3 nucleotides AMB_RS00120 and AMB_RS00125 are separated by 29 nucleotides
AMB_RS00110: AMB_RS00110 - GTP cyclohydrolase II, at 20,317 to 21,429
_RS00110
AMB_RS00115: AMB_RS00115 - hypothetical protein, at 21,454 to 22,608
_RS00115
AMB_RS00120: AMB_RS00120 - YggS family pyridoxal phosphate-dependent enzyme, at 22,612 to 23,262
_RS00120
AMB_RS00125: AMB_RS00125 - thiamine phosphate synthase, at 23,292 to 23,891
_RS00125
Position (kb) 21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 21.567 kb on + strand at 21.567 kb on + strand at 21.568 kb on - strand at 21.592 kb on + strand, within AMB_RS00115 at 21.681 kb on + strand, within AMB_RS00115 at 21.681 kb on + strand, within AMB_RS00115 at 21.681 kb on + strand, within AMB_RS00115 at 21.682 kb on - strand, within AMB_RS00115 at 21.799 kb on + strand, within AMB_RS00115 at 21.800 kb on - strand, within AMB_RS00115 at 21.820 kb on - strand, within AMB_RS00115 at 21.830 kb on + strand, within AMB_RS00115 at 21.831 kb on - strand, within AMB_RS00115 at 21.831 kb on - strand, within AMB_RS00115 at 22.610 kb on - strand at 22.701 kb on + strand, within AMB_RS00120 at 22.744 kb on + strand, within AMB_RS00120 at 22.745 kb on - strand, within AMB_RS00120 at 23.240 kb on - strand at 23.240 kb on - strand at 23.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 21,567 + -1.4 21,567 + -0.0 21,568 - +0.4 21,592 + AMB_RS00115 0.12 +0.2 21,681 + AMB_RS00115 0.20 -3.4 21,681 + AMB_RS00115 0.20 -1.6 21,681 + AMB_RS00115 0.20 +1.8 21,682 - AMB_RS00115 0.20 -0.2 21,799 + AMB_RS00115 0.30 -1.6 21,800 - AMB_RS00115 0.30 +0.8 21,820 - AMB_RS00115 0.32 -0.2 21,830 + AMB_RS00115 0.33 -2.5 21,831 - AMB_RS00115 0.33 +0.2 21,831 - AMB_RS00115 0.33 +0.6 22,610 - -2.3 22,701 + AMB_RS00120 0.14 +0.4 22,744 + AMB_RS00120 0.20 -0.2 22,745 - AMB_RS00120 0.20 -0.1 23,240 - +0.7 23,240 - -0.5 23,281 - +0.8
Or see this region's nucleotide sequence