Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnI and phnH overlap by 1 nucleotides phnH and phnG overlap by 4 nucleotides phnG and phnF are separated by 0 nucleotides
b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686
phnI
b4100: phnH - carbon-phosphorus lyase complex subunit (NCBI), at 4,318,686 to 4,319,270
phnH
b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719
phnG
b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445
phnF
Position (kb)
4318
4319
4320 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4317.686 kb on + strand at 4317.705 kb on + strand at 4317.708 kb on + strand at 4317.708 kb on + strand at 4317.853 kb on + strand, within phnI at 4317.854 kb on - strand, within phnI at 4317.980 kb on + strand, within phnI at 4318.053 kb on + strand, within phnI at 4318.053 kb on + strand, within phnI at 4318.081 kb on - strand, within phnI at 4318.081 kb on - strand, within phnI at 4318.138 kb on - strand, within phnI at 4318.217 kb on - strand, within phnI at 4318.321 kb on - strand, within phnI at 4318.326 kb on - strand, within phnI at 4318.392 kb on + strand, within phnI at 4318.400 kb on + strand, within phnI at 4318.489 kb on - strand, within phnI at 4318.549 kb on + strand, within phnI at 4318.635 kb on - strand at 4318.635 kb on - strand at 4318.687 kb on - strand at 4318.702 kb on - strand at 4318.748 kb on - strand, within phnH at 4318.799 kb on - strand, within phnH at 4318.814 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4319.165 kb on + strand, within phnH at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.228 kb on + strand at 4319.258 kb on + strand at 4319.258 kb on + strand at 4319.259 kb on + strand at 4319.274 kb on - strand at 4319.279 kb on - strand at 4319.279 kb on - strand at 4319.354 kb on - strand, within phnG at 4319.366 kb on - strand, within phnG at 4319.379 kb on - strand, within phnG at 4319.434 kb on - strand, within phnG at 4319.446 kb on - strand, within phnG at 4319.573 kb on + strand, within phnG at 4319.731 kb on - strand at 4319.731 kb on - strand at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.985 kb on - strand, within phnF at 4319.999 kb on + strand, within phnF at 4320.073 kb on - strand, within phnF at 4320.121 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 4,317,686 + -0.3 4,317,705 + -2.1 4,317,708 + -0.4 4,317,708 + -0.4 4,317,853 + phnI b4099 0.22 -2.4 4,317,854 - phnI b4099 0.22 +0.6 4,317,980 + phnI b4099 0.34 +0.8 4,318,053 + phnI b4099 0.40 +0.1 4,318,053 + phnI b4099 0.40 -0.4 4,318,081 - phnI b4099 0.43 +0.1 4,318,081 - phnI b4099 0.43 -1.3 4,318,138 - phnI b4099 0.48 +0.5 4,318,217 - phnI b4099 0.56 -0.6 4,318,321 - phnI b4099 0.66 +0.6 4,318,326 - phnI b4099 0.66 +0.6 4,318,392 + phnI b4099 0.72 +0.4 4,318,400 + phnI b4099 0.73 +1.4 4,318,489 - phnI b4099 0.81 +0.8 4,318,549 + phnI b4099 0.87 -0.8 4,318,635 - +1.4 4,318,635 - +0.8 4,318,687 - +0.1 4,318,702 - -0.0 4,318,748 - phnH b4100 0.11 +2.0 4,318,799 - phnH b4100 0.19 +0.6 4,318,814 + phnH b4100 0.22 -0.1 4,318,855 + phnH b4100 0.29 -1.2 4,318,855 + phnH b4100 0.29 +1.9 4,319,165 + phnH b4100 0.82 +1.4 4,319,223 + +0.2 4,319,223 + -0.2 4,319,223 + +0.0 4,319,228 + +0.2 4,319,258 + -1.0 4,319,258 + +0.2 4,319,259 + +0.9 4,319,274 - -0.2 4,319,279 - +1.0 4,319,279 - +0.6 4,319,354 - phnG b4101 0.19 -0.7 4,319,366 - phnG b4101 0.22 -1.3 4,319,379 - phnG b4101 0.25 -1.7 4,319,434 - phnG b4101 0.37 +0.0 4,319,446 - phnG b4101 0.40 -0.6 4,319,573 + phnG b4101 0.68 +2.2 4,319,731 - +1.5 4,319,731 - -1.1 4,319,803 + phnF b4102 0.11 +2.5 4,319,803 + phnF b4102 0.11 +1.6 4,319,803 + phnF b4102 0.11 +1.8 4,319,811 - phnF b4102 0.13 -1.2 4,319,811 - phnF b4102 0.13 +1.0 4,319,985 - phnF b4102 0.37 +1.0 4,319,999 + phnF b4102 0.38 -0.7 4,320,073 - phnF b4102 0.49 -1.5 4,320,121 - phnF b4102 0.55 +0.7 4,320,253 - phnF b4102 0.73 +1.8 4,320,253 - phnF b4102 0.73 +0.3
Or see this region's nucleotide sequence