Strain Fitness in Escherichia coli BW25113 around b3003

Experiment: Aluminum chloride 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyghZ and yqhA are separated by 39 nucleotidesyqhA and yghA are separated by 190 nucleotidesyghA and exbD are separated by 271 nucleotidesexbD and exbB are separated by 6 nucleotides b3001: yghZ - aldo-keto reductase (NCBI), at 3,145,919 to 3,146,959 yghZ b3002: yqhA - hypothetical protein (NCBI), at 3,146,999 to 3,147,493 yqhA b3003: yghA - oxidoreductase (NCBI), at 3,147,684 to 3,148,568 yghA b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265 exbD b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006 exbB Position (kb) 3147 3148 3149Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3146.753 kb on - strand, within yghZat 3146.753 kb on - strand, within yghZat 3146.753 kb on - strand, within yghZat 3146.840 kb on + strand, within yghZat 3146.842 kb on + strand, within yghZat 3146.842 kb on + strand, within yghZat 3146.863 kb on + strandat 3146.863 kb on + strandat 3146.869 kb on - strandat 3146.971 kb on - strandat 3146.995 kb on - strandat 3147.027 kb on + strandat 3147.309 kb on - strand, within yqhAat 3147.435 kb on - strand, within yqhAat 3147.435 kb on - strand, within yqhAat 3147.461 kb on + strandat 3147.526 kb on + strandat 3147.526 kb on + strandat 3147.531 kb on - strandat 3147.538 kb on + strandat 3147.538 kb on + strandat 3147.540 kb on + strandat 3147.540 kb on + strandat 3147.650 kb on - strandat 3147.713 kb on + strandat 3147.713 kb on + strandat 3147.713 kb on + strandat 3147.723 kb on + strandat 3147.783 kb on - strand, within yghAat 3147.865 kb on - strand, within yghAat 3147.865 kb on - strand, within yghAat 3147.981 kb on + strand, within yghAat 3147.993 kb on - strand, within yghAat 3147.993 kb on - strand, within yghAat 3148.064 kb on - strand, within yghAat 3148.064 kb on - strand, within yghAat 3148.078 kb on - strand, within yghAat 3148.175 kb on + strand, within yghAat 3148.175 kb on + strand, within yghAat 3148.175 kb on + strand, within yghAat 3148.175 kb on + strand, within yghAat 3148.183 kb on - strand, within yghAat 3148.183 kb on - strand, within yghAat 3148.226 kb on + strand, within yghAat 3148.250 kb on - strand, within yghAat 3148.259 kb on - strand, within yghAat 3148.282 kb on - strand, within yghAat 3148.282 kb on - strand, within yghAat 3148.299 kb on + strand, within yghAat 3148.299 kb on + strand, within yghAat 3148.371 kb on + strand, within yghAat 3148.384 kb on - strand, within yghAat 3148.384 kb on - strand, within yghAat 3148.386 kb on + strand, within yghAat 3148.386 kb on + strand, within yghAat 3148.387 kb on - strand, within yghAat 3148.387 kb on - strand, within yghAat 3148.515 kb on + strandat 3148.519 kb on + strandat 3148.540 kb on - strandat 3148.552 kb on - strandat 3148.611 kb on - strandat 3148.694 kb on + strandat 3148.700 kb on - strandat 3148.758 kb on - strandat 3148.758 kb on - strandat 3148.875 kb on - strandat 3148.906 kb on + strand, within exbDat 3148.936 kb on - strand, within exbDat 3148.992 kb on - strand, within exbDat 3149.036 kb on + strand, within exbDat 3149.092 kb on + strand, within exbDat 3149.120 kb on - strandat 3149.120 kb on - strand, within exbDat 3149.131 kb on + strand, within exbDat 3149.131 kb on + strand, within exbDat 3149.208 kb on + strand, within exbDat 3149.377 kb on + strand, within exbBat 3149.377 kb on + strand, within exbBat 3149.380 kb on + strand, within exbBat 3149.396 kb on - strand, within exbBat 3149.448 kb on - strand, within exbBat 3149.501 kb on + strand, within exbBat 3149.508 kb on + strand, within exbBat 3149.543 kb on - strand, within exbBat 3149.553 kb on - strand, within exbBat 3149.554 kb on + strand, within exbBat 3149.564 kb on + strand, within exbB

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM
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3,146,753 - yghZ b3001 0.80 +1.1
3,146,753 - yghZ b3001 0.80 +1.0
3,146,753 - yghZ b3001 0.80 +0.6
3,146,840 + yghZ b3001 0.88 +1.0
3,146,842 + yghZ b3001 0.89 +1.7
3,146,842 + yghZ b3001 0.89 +0.3
3,146,863 + -0.9
3,146,863 + +0.4
3,146,869 - +0.8
3,146,971 - +1.1
3,146,995 - -0.1
3,147,027 + +1.4
3,147,309 - yqhA b3002 0.63 -1.0
3,147,435 - yqhA b3002 0.88 +0.3
3,147,435 - yqhA b3002 0.88 -0.5
3,147,461 + -1.3
3,147,526 + -1.3
3,147,526 + +2.0
3,147,531 - +2.4
3,147,538 + -0.3
3,147,538 + +0.3
3,147,540 + -1.7
3,147,540 + -2.2
3,147,650 - -1.0
3,147,713 + +0.8
3,147,713 + +0.5
3,147,713 + +0.8
3,147,723 + +0.5
3,147,783 - yghA b3003 0.11 +1.0
3,147,865 - yghA b3003 0.20 -0.3
3,147,865 - yghA b3003 0.20 +1.5
3,147,981 + yghA b3003 0.34 -1.1
3,147,993 - yghA b3003 0.35 +0.5
3,147,993 - yghA b3003 0.35 -0.7
3,148,064 - yghA b3003 0.43 -0.7
3,148,064 - yghA b3003 0.43 +1.9
3,148,078 - yghA b3003 0.45 -1.5
3,148,175 + yghA b3003 0.55 -1.3
3,148,175 + yghA b3003 0.55 +0.3
3,148,175 + yghA b3003 0.55 +0.2
3,148,175 + yghA b3003 0.55 -0.1
3,148,183 - yghA b3003 0.56 -1.0
3,148,183 - yghA b3003 0.56 +1.1
3,148,226 + yghA b3003 0.61 +1.3
3,148,250 - yghA b3003 0.64 -1.8
3,148,259 - yghA b3003 0.65 +0.7
3,148,282 - yghA b3003 0.68 +1.4
3,148,282 - yghA b3003 0.68 +2.5
3,148,299 + yghA b3003 0.69 +0.5
3,148,299 + yghA b3003 0.69 +1.3
3,148,371 + yghA b3003 0.78 +1.7
3,148,384 - yghA b3003 0.79 +1.1
3,148,384 - yghA b3003 0.79 -1.7
3,148,386 + yghA b3003 0.79 +0.7
3,148,386 + yghA b3003 0.79 -0.2
3,148,387 - yghA b3003 0.79 +1.9
3,148,387 - yghA b3003 0.79 +0.1
3,148,515 + +1.1
3,148,519 + -0.3
3,148,540 - -2.0
3,148,552 - -1.3
3,148,611 - -0.0
3,148,694 + -1.7
3,148,700 - -0.3
3,148,758 - -0.7
3,148,758 - -0.7
3,148,875 - +0.6
3,148,906 + exbD b3005 0.15 -1.5
3,148,936 - exbD b3005 0.23 -0.3
3,148,992 - exbD b3005 0.36 +0.8
3,149,036 + exbD b3005 0.46 -0.3
3,149,092 + exbD b3005 0.59 +0.2
3,149,120 - +0.3
3,149,120 - exbD b3005 0.66 -0.3
3,149,131 + exbD b3005 0.68 +0.8
3,149,131 + exbD b3005 0.68 +1.3
3,149,208 + exbD b3005 0.86 -0.0
3,149,377 + exbB b3006 0.14 -0.0
3,149,377 + exbB b3006 0.14 -0.4
3,149,380 + exbB b3006 0.15 -0.2
3,149,396 - exbB b3006 0.17 -0.8
3,149,448 - exbB b3006 0.24 +2.1
3,149,501 + exbB b3006 0.31 +3.1
3,149,508 + exbB b3006 0.32 -0.3
3,149,543 - exbB b3006 0.37 -1.3
3,149,553 - exbB b3006 0.38 +1.7
3,149,554 + exbB b3006 0.38 -0.1
3,149,564 + exbB b3006 0.40 +0.6

Or see this region's nucleotide sequence