Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menD and menF are separated by 88 nucleotides menF and elaB are separated by 78 nucleotides elaB and elaA are separated by 54 nucleotides elaA and elaC are separated by 64 nucleotides
b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281
menD
b2265: menF - isochorismate hydroxymutase 2, menaquinone biosynthesis (VIMSS), at 2,377,370 to 2,378,665
menF
b2266: elaB - hypothetical protein (NCBI), at 2,378,744 to 2,379,049
elaB
b2267: elaA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 2,379,104 to 2,379,565
elaA
b2268: elaC - orf, hypothetical protein (VIMSS), at 2,379,630 to 2,380,547
elaC
Position (kb)
2377
2378
2379 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2376.515 kb on + strand, within menD at 2376.554 kb on - strand, within menD at 2376.585 kb on + strand, within menD at 2376.585 kb on + strand, within menD at 2376.605 kb on + strand, within menD at 2376.790 kb on + strand, within menD at 2376.798 kb on - strand, within menD at 2376.798 kb on - strand, within menD at 2377.105 kb on + strand, within menD at 2377.105 kb on + strand, within menD at 2377.151 kb on - strand at 2377.217 kb on - strand at 2377.217 kb on - strand at 2377.232 kb on - strand at 2377.294 kb on + strand at 2377.312 kb on + strand at 2377.320 kb on - strand at 2377.320 kb on - strand at 2377.320 kb on - strand at 2377.398 kb on + strand at 2377.451 kb on + strand at 2377.531 kb on + strand, within menF at 2377.590 kb on - strand, within menF at 2377.590 kb on - strand, within menF at 2377.662 kb on - strand, within menF at 2377.692 kb on + strand, within menF at 2377.692 kb on + strand, within menF at 2377.692 kb on + strand, within menF at 2377.814 kb on - strand, within menF at 2377.851 kb on + strand, within menF at 2377.901 kb on - strand, within menF at 2378.222 kb on + strand, within menF at 2378.224 kb on + strand, within menF at 2378.261 kb on + strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.269 kb on - strand, within menF at 2378.351 kb on + strand, within menF at 2378.375 kb on - strand, within menF at 2378.375 kb on - strand, within menF at 2378.425 kb on + strand, within menF at 2378.425 kb on + strand, within menF at 2378.487 kb on + strand, within menF at 2378.530 kb on + strand, within menF at 2378.543 kb on - strand at 2378.731 kb on + strand at 2378.765 kb on + strand at 2378.765 kb on + strand at 2378.840 kb on + strand, within elaB at 2378.861 kb on + strand, within elaB at 2378.983 kb on + strand, within elaB at 2379.012 kb on - strand, within elaB at 2379.012 kb on - strand, within elaB at 2379.073 kb on + strand at 2379.073 kb on + strand at 2379.092 kb on - strand at 2379.092 kb on - strand at 2379.152 kb on - strand, within elaA at 2379.161 kb on - strand, within elaA at 2379.161 kb on - strand, within elaA at 2379.251 kb on - strand, within elaA at 2379.284 kb on - strand, within elaA at 2379.452 kb on + strand, within elaA at 2379.485 kb on + strand, within elaA at 2379.540 kb on - strand at 2379.542 kb on + strand at 2379.569 kb on - strand at 2379.569 kb on - strand at 2379.588 kb on + strand at 2379.588 kb on + strand at 2379.655 kb on + strand at 2379.663 kb on + strand at 2379.663 kb on + strand at 2379.663 kb on + strand at 2379.663 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 2,376,515 + menD b2264 0.54 +1.3 2,376,554 - menD b2264 0.56 -2.4 2,376,585 + menD b2264 0.58 +2.2 2,376,585 + menD b2264 0.58 +0.7 2,376,605 + menD b2264 0.59 +1.4 2,376,790 + menD b2264 0.71 +2.0 2,376,798 - menD b2264 0.71 -0.2 2,376,798 - menD b2264 0.71 -1.4 2,377,105 + menD b2264 0.89 -1.2 2,377,105 + menD b2264 0.89 -1.0 2,377,151 - +1.0 2,377,217 - +0.4 2,377,217 - +0.8 2,377,232 - -0.0 2,377,294 + +1.1 2,377,312 + +1.2 2,377,320 - +0.5 2,377,320 - +0.7 2,377,320 - -1.0 2,377,398 + +0.4 2,377,451 + -0.9 2,377,531 + menF b2265 0.12 +0.2 2,377,590 - menF b2265 0.17 +0.5 2,377,590 - menF b2265 0.17 +1.0 2,377,662 - menF b2265 0.23 -1.6 2,377,692 + menF b2265 0.25 -0.5 2,377,692 + menF b2265 0.25 -0.6 2,377,692 + menF b2265 0.25 -0.8 2,377,814 - menF b2265 0.34 -0.9 2,377,851 + menF b2265 0.37 +0.1 2,377,901 - menF b2265 0.41 -1.2 2,378,222 + menF b2265 0.66 -1.1 2,378,224 + menF b2265 0.66 +0.4 2,378,261 + menF b2265 0.69 -0.7 2,378,269 - menF b2265 0.69 -0.6 2,378,269 - menF b2265 0.69 -2.0 2,378,269 - menF b2265 0.69 -0.7 2,378,269 - menF b2265 0.69 -0.6 2,378,269 - menF b2265 0.69 -2.2 2,378,351 + menF b2265 0.76 +0.6 2,378,375 - menF b2265 0.78 -2.0 2,378,375 - menF b2265 0.78 -2.4 2,378,425 + menF b2265 0.81 +0.5 2,378,425 + menF b2265 0.81 +0.7 2,378,487 + menF b2265 0.86 +0.4 2,378,530 + menF b2265 0.90 -0.4 2,378,543 - -0.5 2,378,731 + -0.5 2,378,765 + +0.3 2,378,765 + +1.2 2,378,840 + elaB b2266 0.31 -0.5 2,378,861 + elaB b2266 0.38 -1.8 2,378,983 + elaB b2266 0.78 +0.1 2,379,012 - elaB b2266 0.88 +0.7 2,379,012 - elaB b2266 0.88 +0.5 2,379,073 + +1.4 2,379,073 + -0.6 2,379,092 - -0.0 2,379,092 - -1.0 2,379,152 - elaA b2267 0.10 -1.6 2,379,161 - elaA b2267 0.12 +1.4 2,379,161 - elaA b2267 0.12 -2.7 2,379,251 - elaA b2267 0.32 +0.4 2,379,284 - elaA b2267 0.39 -0.1 2,379,452 + elaA b2267 0.75 +0.4 2,379,485 + elaA b2267 0.82 -2.6 2,379,540 - +1.7 2,379,542 + -0.9 2,379,569 - +2.4 2,379,569 - +1.3 2,379,588 + +1.8 2,379,588 + +0.4 2,379,655 + +0.7 2,379,663 + -0.6 2,379,663 + -0.5 2,379,663 + -1.2 2,379,663 + +2.5
Or see this region's nucleotide sequence