Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoC and atoD are separated by 195 nucleotides atoD and atoA overlap by 1 nucleotides atoA and atoE overlap by 4 nucleotides
b2220: atoC - fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI), at 2,319,888 to 2,321,273
atoC
b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131
atoD
b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781
atoA
b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100
atoE
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2320.600 kb on + strand, within atoC at 2320.640 kb on + strand, within atoC at 2320.641 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.671 kb on - strand, within atoC at 2320.674 kb on - strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.833 kb on - strand, within atoC at 2320.833 kb on - strand, within atoC at 2320.936 kb on + strand, within atoC at 2320.936 kb on + strand, within atoC at 2320.938 kb on + strand, within atoC at 2320.944 kb on - strand, within atoC at 2320.944 kb on - strand, within atoC at 2320.972 kb on + strand, within atoC at 2320.972 kb on + strand, within atoC at 2321.007 kb on + strand, within atoC at 2321.014 kb on + strand, within atoC at 2321.179 kb on - strand at 2321.194 kb on - strand at 2321.196 kb on + strand at 2321.196 kb on + strand at 2321.236 kb on - strand at 2321.261 kb on + strand at 2321.266 kb on + strand at 2321.496 kb on - strand at 2321.519 kb on - strand at 2321.700 kb on + strand, within atoD at 2321.707 kb on - strand, within atoD at 2321.818 kb on + strand, within atoD at 2321.967 kb on + strand, within atoD at 2322.085 kb on - strand at 2322.255 kb on - strand, within atoA at 2322.362 kb on - strand, within atoA at 2322.391 kb on + strand, within atoA at 2322.410 kb on - strand, within atoA at 2322.423 kb on - strand, within atoA at 2322.629 kb on - strand, within atoA at 2322.892 kb on + strand at 2322.927 kb on + strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.999 kb on + strand, within atoE at 2323.113 kb on - strand, within atoE
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 2,320,600 + atoC b2220 0.51 +1.1 2,320,640 + atoC b2220 0.54 -2.8 2,320,641 + atoC b2220 0.54 -0.1 2,320,668 + atoC b2220 0.56 -0.5 2,320,668 + atoC b2220 0.56 -0.8 2,320,671 - atoC b2220 0.56 +1.3 2,320,674 - atoC b2220 0.57 +0.6 2,320,752 + atoC b2220 0.62 -1.6 2,320,752 + atoC b2220 0.62 -0.4 2,320,833 - atoC b2220 0.68 -1.9 2,320,833 - atoC b2220 0.68 +1.2 2,320,936 + atoC b2220 0.76 +1.3 2,320,936 + atoC b2220 0.76 +0.1 2,320,938 + atoC b2220 0.76 -0.7 2,320,944 - atoC b2220 0.76 +0.7 2,320,944 - atoC b2220 0.76 +0.3 2,320,972 + atoC b2220 0.78 -0.8 2,320,972 + atoC b2220 0.78 -0.5 2,321,007 + atoC b2220 0.81 -0.1 2,321,014 + atoC b2220 0.81 -0.5 2,321,179 - +1.7 2,321,194 - +0.4 2,321,196 + -0.6 2,321,196 + -1.6 2,321,236 - +0.5 2,321,261 + -0.1 2,321,266 + -0.4 2,321,496 - +0.4 2,321,519 - +0.3 2,321,700 + atoD b2221 0.35 -0.6 2,321,707 - atoD b2221 0.36 -3.2 2,321,818 + atoD b2221 0.53 +0.1 2,321,967 + atoD b2221 0.75 -2.8 2,322,085 - +0.9 2,322,255 - atoA b2222 0.19 -0.5 2,322,362 - atoA b2222 0.35 +0.0 2,322,391 + atoA b2222 0.40 -0.8 2,322,410 - atoA b2222 0.43 -0.8 2,322,423 - atoA b2222 0.45 +1.3 2,322,629 - atoA b2222 0.76 +3.7 2,322,892 + -0.5 2,322,927 + atoE b2223 0.11 -2.4 2,322,978 - atoE b2223 0.15 -0.1 2,322,978 - atoE b2223 0.15 +0.5 2,322,999 + atoE b2223 0.17 -1.1 2,323,113 - atoE b2223 0.25 -2.0
Or see this region's nucleotide sequence