Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaF and wcaE are separated by 15 nucleotides wcaE and wcaD are separated by 10 nucleotides wcaD and wcaC overlap by 26 nucleotides
b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912
wcaF
b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674
wcaE
b2056: wcaD - predicted colanic acid polymerase (NCBI), at 2,127,685 to 2,128,902
wcaD
b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094
wcaC
Position (kb)
2127
2128
2129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2126.914 kb on + strand at 2127.243 kb on - strand, within wcaE at 2127.467 kb on - strand, within wcaE at 2127.583 kb on + strand, within wcaE at 2127.584 kb on + strand, within wcaE at 2127.617 kb on + strand at 2127.663 kb on + strand at 2127.663 kb on + strand at 2128.019 kb on - strand, within wcaD at 2128.137 kb on - strand, within wcaD at 2128.137 kb on - strand, within wcaD at 2128.146 kb on - strand, within wcaD at 2128.222 kb on - strand, within wcaD at 2128.251 kb on - strand, within wcaD at 2128.259 kb on - strand, within wcaD at 2128.259 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.280 kb on - strand, within wcaD at 2128.299 kb on - strand, within wcaD at 2128.304 kb on - strand, within wcaD at 2128.309 kb on - strand, within wcaD at 2128.324 kb on - strand, within wcaD at 2128.324 kb on - strand, within wcaD at 2128.355 kb on - strand, within wcaD at 2128.439 kb on - strand, within wcaD at 2128.439 kb on - strand, within wcaD at 2128.475 kb on - strand, within wcaD at 2128.527 kb on + strand, within wcaD at 2128.527 kb on + strand, within wcaD at 2128.530 kb on - strand, within wcaD at 2128.530 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.531 kb on - strand, within wcaD at 2128.535 kb on - strand, within wcaD at 2128.535 kb on - strand, within wcaD at 2128.545 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.604 kb on + strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.612 kb on - strand, within wcaD at 2128.614 kb on + strand, within wcaD at 2128.617 kb on + strand, within wcaD at 2128.667 kb on - strand, within wcaD at 2128.668 kb on - strand, within wcaD at 2128.690 kb on - strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.696 kb on + strand, within wcaD at 2128.702 kb on - strand, within wcaD at 2128.704 kb on - strand, within wcaD at 2128.713 kb on - strand, within wcaD at 2128.722 kb on + strand, within wcaD at 2128.918 kb on - strand at 2128.918 kb on - strand at 2128.925 kb on + strand at 2128.957 kb on + strand at 2128.968 kb on + strand at 2129.087 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.468 kb on + strand, within wcaC at 2129.473 kb on + strand, within wcaC at 2129.586 kb on + strand, within wcaC at 2129.690 kb on + strand, within wcaC at 2129.798 kb on + strand, within wcaC at 2129.803 kb on + strand, within wcaC
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 2,126,914 + +0.2 2,127,243 - wcaE b2055 0.42 -0.5 2,127,467 - wcaE b2055 0.72 -2.6 2,127,583 + wcaE b2055 0.88 +0.9 2,127,584 + wcaE b2055 0.88 -0.1 2,127,617 + +0.1 2,127,663 + +0.9 2,127,663 + -2.7 2,128,019 - wcaD b2056 0.27 -0.2 2,128,137 - wcaD b2056 0.37 +0.2 2,128,137 - wcaD b2056 0.37 -2.2 2,128,146 - wcaD b2056 0.38 -2.6 2,128,222 - wcaD b2056 0.44 -0.4 2,128,251 - wcaD b2056 0.46 -0.2 2,128,259 - wcaD b2056 0.47 +0.3 2,128,259 - wcaD b2056 0.47 -1.0 2,128,280 - wcaD b2056 0.49 -1.7 2,128,280 - wcaD b2056 0.49 -1.9 2,128,280 - wcaD b2056 0.49 -0.4 2,128,299 - wcaD b2056 0.50 -0.3 2,128,304 - wcaD b2056 0.51 -0.1 2,128,309 - wcaD b2056 0.51 +0.1 2,128,324 - wcaD b2056 0.52 -0.7 2,128,324 - wcaD b2056 0.52 -0.4 2,128,355 - wcaD b2056 0.55 +0.8 2,128,439 - wcaD b2056 0.62 -1.7 2,128,439 - wcaD b2056 0.62 -0.8 2,128,475 - wcaD b2056 0.65 -3.1 2,128,527 + wcaD b2056 0.69 -1.4 2,128,527 + wcaD b2056 0.69 +0.7 2,128,530 - wcaD b2056 0.69 -0.4 2,128,530 - wcaD b2056 0.69 -1.1 2,128,531 - wcaD b2056 0.69 -1.9 2,128,531 - wcaD b2056 0.69 -0.9 2,128,531 - wcaD b2056 0.69 -0.1 2,128,531 - wcaD b2056 0.69 +0.4 2,128,535 - wcaD b2056 0.70 -0.2 2,128,535 - wcaD b2056 0.70 -0.9 2,128,545 + wcaD b2056 0.71 -3.3 2,128,604 + wcaD b2056 0.75 +1.4 2,128,604 + wcaD b2056 0.75 +0.7 2,128,604 + wcaD b2056 0.75 -0.8 2,128,604 + wcaD b2056 0.75 -0.7 2,128,612 - wcaD b2056 0.76 +0.5 2,128,612 - wcaD b2056 0.76 -1.4 2,128,612 - wcaD b2056 0.76 +0.1 2,128,614 + wcaD b2056 0.76 -0.6 2,128,617 + wcaD b2056 0.77 +0.5 2,128,667 - wcaD b2056 0.81 +1.9 2,128,668 - wcaD b2056 0.81 -0.8 2,128,690 - wcaD b2056 0.83 +1.3 2,128,696 + wcaD b2056 0.83 +1.6 2,128,696 + wcaD b2056 0.83 +0.7 2,128,696 + wcaD b2056 0.83 -0.7 2,128,702 - wcaD b2056 0.83 -0.4 2,128,704 - wcaD b2056 0.84 -0.4 2,128,713 - wcaD b2056 0.84 +0.5 2,128,722 + wcaD b2056 0.85 -2.3 2,128,918 - -0.1 2,128,918 - -0.7 2,128,925 + -1.3 2,128,957 + -2.2 2,128,968 + -3.3 2,129,087 - wcaC b2057 0.17 -1.4 2,129,249 - wcaC b2057 0.31 -1.1 2,129,249 - wcaC b2057 0.31 +1.5 2,129,459 + wcaC b2057 0.48 -1.7 2,129,459 + wcaC b2057 0.48 +0.5 2,129,468 + wcaC b2057 0.49 +1.9 2,129,473 + wcaC b2057 0.49 +0.4 2,129,586 + wcaC b2057 0.58 -1.8 2,129,690 + wcaC b2057 0.67 +3.4 2,129,798 + wcaC b2057 0.76 -0.1 2,129,803 + wcaC b2057 0.76 -0.1
Or see this region's nucleotide sequence