Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malX and malY are separated by 9 nucleotides malY and add are separated by 103 nucleotides
b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971
malX
b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153
malY
b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258
add
Position (kb)
1698
1699
1700
1701 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1698.219 kb on - strand, within malX at 1698.303 kb on + strand, within malX at 1698.450 kb on + strand, within malX at 1698.450 kb on + strand, within malX at 1698.495 kb on + strand, within malX at 1698.716 kb on - strand, within malX at 1698.730 kb on - strand, within malX at 1698.918 kb on - strand at 1698.950 kb on + strand at 1698.950 kb on + strand at 1698.952 kb on + strand at 1699.029 kb on - strand at 1699.060 kb on + strand at 1699.068 kb on - strand at 1699.068 kb on - strand at 1699.152 kb on + strand, within malY at 1699.279 kb on + strand, within malY at 1699.279 kb on + strand, within malY at 1699.546 kb on + strand, within malY at 1699.546 kb on + strand, within malY at 1699.566 kb on - strand, within malY at 1699.796 kb on + strand, within malY at 1700.152 kb on + strand at 1700.214 kb on + strand at 1700.313 kb on - strand at 1700.327 kb on + strand at 1700.572 kb on - strand, within add at 1700.727 kb on + strand, within add at 1700.929 kb on - strand, within add at 1701.020 kb on + strand, within add at 1701.061 kb on - strand, within add at 1701.086 kb on - strand, within add at 1701.086 kb on - strand, within add at 1701.146 kb on + strand, within add
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 1,698,219 - malX b1621 0.53 -0.6 1,698,303 + malX b1621 0.58 -1.2 1,698,450 + malX b1621 0.67 +0.7 1,698,450 + malX b1621 0.67 +0.7 1,698,495 + malX b1621 0.70 +1.4 1,698,716 - malX b1621 0.84 +0.2 1,698,730 - malX b1621 0.85 +0.3 1,698,918 - +0.7 1,698,950 + +2.3 1,698,950 + +0.7 1,698,952 + -1.3 1,699,029 - +0.2 1,699,060 + -1.3 1,699,068 - -1.3 1,699,068 - +0.6 1,699,152 + malY b1622 0.15 +0.7 1,699,279 + malY b1622 0.25 +0.2 1,699,279 + malY b1622 0.25 -2.3 1,699,546 + malY b1622 0.48 +0.5 1,699,546 + malY b1622 0.48 +0.4 1,699,566 - malY b1622 0.50 +0.4 1,699,796 + malY b1622 0.69 +0.6 1,700,152 + +0.3 1,700,214 + -1.3 1,700,313 - -0.3 1,700,327 + +0.4 1,700,572 - add b1623 0.31 -0.1 1,700,727 + add b1623 0.47 +0.2 1,700,929 - add b1623 0.67 +3.4 1,701,020 + add b1623 0.76 -0.3 1,701,061 - add b1623 0.80 +0.1 1,701,086 - add b1623 0.83 -0.3 1,701,086 - add b1623 0.83 -2.1 1,701,146 + add b1623 0.89 +0.0
Or see this region's nucleotide sequence