Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yncJ and yncN are separated by 221 nucleotides yncN and ydcQ are separated by 45 nucleotides ydcQ and ydcR are separated by 78 nucleotides ydcR and ydcS are separated by 244 nucleotides
b1436: yncJ - hypothetical protein (NCBI), at 1,506,858 to 1,507,088
yncJ
b4532: yncN - orf, hypothetical protein (VIMSS), at 1,507,310 to 1,507,486
yncN
b1438: ydcQ - predicted DNA-binding transcriptional regulator (RefSeq), at 1,507,532 to 1,507,948
ydcQ
b1439: ydcR - fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI), at 1,508,027 to 1,509,433
ydcR
b1440: ydcS - predicted spermidine/putrescine transporter subunit (NCBI), at 1,509,678 to 1,510,823
ydcS
Position (kb)
1508
1509
1510 Strain fitness (log2 ratio)
-1
0
1
2 at 1507.385 kb on - strand, within yncN at 1507.407 kb on + strand, within yncN at 1507.450 kb on + strand, within yncN at 1507.631 kb on + strand, within ydcQ at 1507.675 kb on + strand, within ydcQ at 1507.702 kb on + strand, within ydcQ at 1507.710 kb on - strand, within ydcQ at 1507.761 kb on + strand, within ydcQ at 1507.819 kb on + strand, within ydcQ at 1508.082 kb on + strand at 1508.082 kb on + strand at 1508.230 kb on - strand, within ydcR at 1508.233 kb on - strand, within ydcR at 1508.314 kb on + strand, within ydcR at 1508.314 kb on + strand, within ydcR at 1508.403 kb on + strand, within ydcR at 1508.479 kb on - strand, within ydcR at 1508.509 kb on - strand, within ydcR at 1508.632 kb on + strand, within ydcR at 1508.820 kb on + strand, within ydcR at 1508.820 kb on + strand, within ydcR at 1508.990 kb on - strand, within ydcR at 1509.170 kb on - strand, within ydcR at 1509.170 kb on - strand, within ydcR at 1509.236 kb on + strand, within ydcR at 1509.369 kb on + strand at 1509.375 kb on - strand at 1509.377 kb on - strand at 1509.377 kb on - strand at 1509.674 kb on - strand at 1509.693 kb on + strand at 1509.707 kb on + strand at 1509.718 kb on + strand at 1509.718 kb on + strand at 1509.733 kb on - strand at 1509.809 kb on + strand, within ydcS at 1509.834 kb on + strand, within ydcS at 1509.884 kb on + strand, within ydcS at 1510.004 kb on + strand, within ydcS at 1510.220 kb on + strand, within ydcS at 1510.227 kb on + strand, within ydcS at 1510.336 kb on - strand, within ydcS at 1510.336 kb on - strand, within ydcS at 1510.361 kb on + strand, within ydcS at 1510.390 kb on + strand, within ydcS at 1510.390 kb on + strand, within ydcS at 1510.392 kb on - strand, within ydcS
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 1,507,385 - yncN b4532 0.42 -0.0 1,507,407 + yncN b4532 0.55 +1.7 1,507,450 + yncN b4532 0.79 -0.2 1,507,631 + ydcQ b1438 0.24 +0.5 1,507,675 + ydcQ b1438 0.34 +0.5 1,507,702 + ydcQ b1438 0.41 +0.2 1,507,710 - ydcQ b1438 0.43 +0.7 1,507,761 + ydcQ b1438 0.55 -1.3 1,507,819 + ydcQ b1438 0.69 -1.3 1,508,082 + -1.2 1,508,082 + +0.4 1,508,230 - ydcR b1439 0.14 +0.7 1,508,233 - ydcR b1439 0.15 -0.5 1,508,314 + ydcR b1439 0.20 +2.3 1,508,314 + ydcR b1439 0.20 -0.4 1,508,403 + ydcR b1439 0.27 -0.1 1,508,479 - ydcR b1439 0.32 +0.5 1,508,509 - ydcR b1439 0.34 +0.8 1,508,632 + ydcR b1439 0.43 +1.3 1,508,820 + ydcR b1439 0.56 +1.3 1,508,820 + ydcR b1439 0.56 +2.3 1,508,990 - ydcR b1439 0.68 -0.6 1,509,170 - ydcR b1439 0.81 +1.1 1,509,170 - ydcR b1439 0.81 +0.5 1,509,236 + ydcR b1439 0.86 +2.4 1,509,369 + +1.2 1,509,375 - +0.5 1,509,377 - +1.6 1,509,377 - -0.5 1,509,674 - -0.3 1,509,693 + +0.9 1,509,707 + +1.4 1,509,718 + +0.4 1,509,718 + -1.3 1,509,733 - -0.6 1,509,809 + ydcS b1440 0.11 -1.0 1,509,834 + ydcS b1440 0.14 -0.0 1,509,884 + ydcS b1440 0.18 +0.3 1,510,004 + ydcS b1440 0.28 +1.1 1,510,220 + ydcS b1440 0.47 +0.4 1,510,227 + ydcS b1440 0.48 +0.3 1,510,336 - ydcS b1440 0.57 +0.1 1,510,336 - ydcS b1440 0.57 -1.3 1,510,361 + ydcS b1440 0.60 -0.9 1,510,390 + ydcS b1440 0.62 +1.2 1,510,390 + ydcS b1440 0.62 +0.7 1,510,392 - ydcS b1440 0.62 +0.4
Or see this region's nucleotide sequence