Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potF and potG are separated by 94 nucleotides potG and potH are separated by 9 nucleotides potH and potI overlap by 4 nucleotides
b0854: potF - putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 893,007 to 894,119
potF
b0855: potG - ATP-binding component of putrescine transport system (VIMSS), at 894,214 to 895,347
potG
b0856: potH - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 895,357 to 896,310
potH
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
Position (kb) 894
895
896 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 893.261 kb on + strand, within potF at 893.352 kb on - strand, within potF at 893.362 kb on - strand, within potF at 893.400 kb on + strand, within potF at 893.408 kb on - strand, within potF at 893.408 kb on - strand, within potF at 893.490 kb on - strand, within potF at 893.696 kb on - strand, within potF at 894.044 kb on - strand at 894.044 kb on - strand at 894.323 kb on + strand at 894.396 kb on + strand, within potG at 894.541 kb on - strand, within potG at 894.691 kb on - strand, within potG at 894.746 kb on - strand, within potG at 894.755 kb on + strand, within potG at 894.755 kb on + strand, within potG at 894.797 kb on - strand, within potG at 894.816 kb on + strand, within potG at 894.847 kb on - strand, within potG at 894.877 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.929 kb on - strand, within potG at 895.067 kb on + strand, within potG at 895.099 kb on - strand, within potG at 895.099 kb on - strand, within potG at 895.246 kb on + strand at 895.325 kb on + strand at 895.325 kb on + strand at 895.461 kb on + strand, within potH at 895.461 kb on + strand, within potH at 895.603 kb on - strand, within potH at 895.694 kb on + strand, within potH at 895.848 kb on + strand, within potH at 895.861 kb on - strand, within potH at 895.930 kb on + strand, within potH at 895.937 kb on - strand, within potH at 895.937 kb on - strand, within potH at 896.095 kb on - strand, within potH
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 893,261 + potF b0854 0.23 +1.0 893,352 - potF b0854 0.31 +1.1 893,362 - potF b0854 0.32 -0.9 893,400 + potF b0854 0.35 +0.2 893,408 - potF b0854 0.36 -0.1 893,408 - potF b0854 0.36 +1.4 893,490 - potF b0854 0.43 +0.2 893,696 - potF b0854 0.62 -0.4 894,044 - +0.1 894,044 - +0.1 894,323 + +0.1 894,396 + potG b0855 0.16 -0.6 894,541 - potG b0855 0.29 -0.9 894,691 - potG b0855 0.42 +2.4 894,746 - potG b0855 0.47 -0.5 894,755 + potG b0855 0.48 -1.5 894,755 + potG b0855 0.48 -0.1 894,797 - potG b0855 0.51 +0.1 894,816 + potG b0855 0.53 -0.2 894,847 - potG b0855 0.56 -1.8 894,877 + potG b0855 0.58 -2.5 894,922 + potG b0855 0.62 +2.1 894,922 + potG b0855 0.62 +0.1 894,929 - potG b0855 0.63 -2.1 895,067 + potG b0855 0.75 +0.3 895,099 - potG b0855 0.78 -1.4 895,099 - potG b0855 0.78 -1.4 895,246 + +1.4 895,325 + +1.6 895,325 + -0.3 895,461 + potH b0856 0.11 +0.8 895,461 + potH b0856 0.11 +2.8 895,603 - potH b0856 0.26 +1.5 895,694 + potH b0856 0.35 +2.1 895,848 + potH b0856 0.51 -0.9 895,861 - potH b0856 0.53 -0.6 895,930 + potH b0856 0.60 -1.3 895,937 - potH b0856 0.61 +0.6 895,937 - potH b0856 0.61 +2.1 896,095 - potH b0856 0.77 +1.2
Or see this region's nucleotide sequence