Experiment: Aluminum chloride 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpA and mhpB are separated by 1 nucleotides mhpB and mhpC are separated by 2 nucleotides mhpC and mhpD are separated by 9 nucleotides mhpD and mhpF overlap by 4 nucleotides
b0347: mhpA - 3-(3-hydroxyphenyl)propionate hydroxylase (NCBI), at 367,835 to 369,499
mhpA
b0348: mhpB - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI), at 369,501 to 370,445
mhpB
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
b0351: mhpF - acetaldehyde dehydrogenase (NCBI), at 372,145 to 373,095
mhpF
Position (kb) 370
371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 369.625 kb on + strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.837 kb on - strand, within mhpB at 369.908 kb on + strand, within mhpB at 369.908 kb on + strand, within mhpB at 370.056 kb on - strand, within mhpB at 370.198 kb on - strand, within mhpB at 370.263 kb on + strand, within mhpB at 370.344 kb on + strand, within mhpB at 370.364 kb on + strand at 370.398 kb on - strand at 370.517 kb on - strand at 370.524 kb on + strand at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.632 kb on + strand, within mhpC at 370.635 kb on - strand, within mhpC at 370.688 kb on - strand, within mhpC at 370.690 kb on + strand, within mhpC at 370.813 kb on + strand, within mhpC at 370.931 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.143 kb on - strand, within mhpC at 371.194 kb on + strand, within mhpC at 371.196 kb on + strand, within mhpC at 371.237 kb on + strand, within mhpC at 371.247 kb on - strand at 371.280 kb on - strand at 371.325 kb on + strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.360 kb on - strand at 371.472 kb on - strand, within mhpD at 371.489 kb on + strand, within mhpD at 371.511 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.530 kb on - strand, within mhpD at 371.753 kb on - strand, within mhpD at 371.791 kb on + strand, within mhpD at 371.815 kb on + strand, within mhpD at 371.936 kb on + strand, within mhpD at 371.943 kb on - strand, within mhpD at 371.982 kb on + strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.999 kb on + strand, within mhpD at 371.999 kb on + strand, within mhpD at 372.009 kb on - strand, within mhpD at 372.028 kb on - strand, within mhpD at 372.181 kb on - strand at 372.181 kb on - strand at 372.191 kb on - strand at 372.191 kb on - strand at 372.193 kb on + strand at 372.193 kb on + strand at 372.226 kb on + strand at 372.226 kb on + strand at 372.271 kb on + strand, within mhpF at 372.307 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 5 mM remove 369,625 + mhpB b0348 0.13 +0.8 369,705 - mhpB b0348 0.22 -0.5 369,705 - mhpB b0348 0.22 -0.5 369,829 + mhpB b0348 0.35 -0.2 369,829 + mhpB b0348 0.35 +3.4 369,837 - mhpB b0348 0.36 +0.1 369,908 + mhpB b0348 0.43 -0.4 369,908 + mhpB b0348 0.43 -0.6 370,056 - mhpB b0348 0.59 +2.1 370,198 - mhpB b0348 0.74 +0.1 370,263 + mhpB b0348 0.81 -0.0 370,344 + mhpB b0348 0.89 +2.1 370,364 + -0.1 370,398 - +0.2 370,517 - -0.4 370,524 + +1.6 370,598 - mhpC b0349 0.17 +1.0 370,598 - mhpC b0349 0.17 -1.9 370,598 - mhpC b0349 0.17 -1.1 370,632 + mhpC b0349 0.21 +1.1 370,635 - mhpC b0349 0.21 +1.7 370,688 - mhpC b0349 0.27 +0.9 370,690 + mhpC b0349 0.27 +0.8 370,813 + mhpC b0349 0.41 -1.2 370,931 - mhpC b0349 0.55 +1.1 371,051 - mhpC b0349 0.68 -2.4 371,051 - mhpC b0349 0.68 +1.9 371,089 + mhpC b0349 0.73 +0.5 371,089 + mhpC b0349 0.73 -1.5 371,143 - mhpC b0349 0.79 -0.0 371,194 + mhpC b0349 0.85 -2.2 371,196 + mhpC b0349 0.85 +0.2 371,237 + mhpC b0349 0.89 +0.2 371,247 - +0.4 371,280 - +0.5 371,325 + +0.6 371,331 - +0.1 371,331 - -0.5 371,331 - -0.7 371,360 - -1.5 371,472 - mhpD b0350 0.16 -0.9 371,489 + mhpD b0350 0.19 -1.2 371,511 + mhpD b0350 0.21 +0.8 371,522 + mhpD b0350 0.23 +2.0 371,522 + mhpD b0350 0.23 -0.9 371,530 - mhpD b0350 0.24 +1.3 371,753 - mhpD b0350 0.51 +1.1 371,791 + mhpD b0350 0.56 +1.6 371,815 + mhpD b0350 0.59 -0.7 371,936 + mhpD b0350 0.74 +1.1 371,943 - mhpD b0350 0.75 +0.4 371,982 + mhpD b0350 0.79 -3.0 371,996 - mhpD b0350 0.81 +1.1 371,996 - mhpD b0350 0.81 +1.4 371,999 + mhpD b0350 0.81 -1.5 371,999 + mhpD b0350 0.81 -1.6 372,009 - mhpD b0350 0.83 +0.6 372,028 - mhpD b0350 0.85 -2.4 372,181 - -1.9 372,181 - -0.9 372,191 - +2.7 372,191 - +0.1 372,193 + +0.4 372,193 + +1.1 372,226 + -1.2 372,226 + +0.9 372,271 + mhpF b0351 0.13 -1.5 372,307 + mhpF b0351 0.17 +0.7 372,308 + mhpF b0351 0.17 +1.2 372,308 + mhpF b0351 0.17 +0.1 372,308 + mhpF b0351 0.17 -0.9
Or see this region's nucleotide sequence