Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09075 and AMB_RS23750 are separated by 360 nucleotides AMB_RS23750 and AMB_RS09080 overlap by 4 nucleotides
AMB_RS09075: AMB_RS09075 - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,932,289 to 1,933,329
_RS09075
AMB_RS23750: AMB_RS23750 - modified peptide precursor CbpA, at 1,933,690 to 1,933,806
_RS23750
AMB_RS09080: AMB_RS09080 - peptide-modifying radical SAM enzyme CbpB, at 1,933,803 to 1,935,182
_RS09080
Position (kb)
1933
1934 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1933.419 kb on + strand at 1933.419 kb on + strand at 1933.424 kb on + strand at 1933.424 kb on + strand at 1933.425 kb on - strand at 1933.425 kb on - strand at 1933.425 kb on - strand at 1933.425 kb on - strand at 1933.425 kb on - strand at 1933.485 kb on + strand at 1933.672 kb on + strand at 1933.673 kb on - strand at 1933.774 kb on + strand, within AMB_RS23750 at 1933.774 kb on + strand, within AMB_RS23750 at 1933.774 kb on + strand, within AMB_RS23750 at 1933.774 kb on + strand, within AMB_RS23750 at 1933.775 kb on - strand, within AMB_RS23750 at 1933.775 kb on - strand, within AMB_RS23750 at 1933.775 kb on - strand, within AMB_RS23750 at 1933.775 kb on - strand, within AMB_RS23750 at 1933.789 kb on - strand, within AMB_RS23750 at 1933.789 kb on - strand, within AMB_RS23750 at 1933.879 kb on - strand at 1934.046 kb on + strand, within AMB_RS09080 at 1934.047 kb on - strand, within AMB_RS09080 at 1934.085 kb on + strand, within AMB_RS09080 at 1934.085 kb on + strand, within AMB_RS09080 at 1934.086 kb on - strand, within AMB_RS09080 at 1934.091 kb on + strand, within AMB_RS09080 at 1934.091 kb on + strand, within AMB_RS09080 at 1934.092 kb on - strand, within AMB_RS09080 at 1934.092 kb on - strand, within AMB_RS09080 at 1934.092 kb on - strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.126 kb on + strand, within AMB_RS09080 at 1934.127 kb on - strand, within AMB_RS09080 at 1934.127 kb on - strand, within AMB_RS09080 at 1934.173 kb on - strand, within AMB_RS09080 at 1934.274 kb on + strand, within AMB_RS09080 at 1934.274 kb on + strand, within AMB_RS09080 at 1934.478 kb on + strand, within AMB_RS09080 at 1934.479 kb on - strand, within AMB_RS09080 at 1934.479 kb on - strand, within AMB_RS09080 at 1934.652 kb on + strand, within AMB_RS09080 at 1934.652 kb on + strand, within AMB_RS09080 at 1934.652 kb on + strand, within AMB_RS09080 at 1934.653 kb on - strand, within AMB_RS09080 at 1934.653 kb on - strand, within AMB_RS09080 at 1934.653 kb on - strand, within AMB_RS09080
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,933,419 + -1.8 1,933,419 + +0.1 1,933,424 + -2.3 1,933,424 + -1.2 1,933,425 - -0.6 1,933,425 - -1.2 1,933,425 - +1.4 1,933,425 - -1.4 1,933,425 - -0.8 1,933,485 + -0.4 1,933,672 + -2.9 1,933,673 - +1.4 1,933,774 + AMB_RS23750 0.72 -1.8 1,933,774 + AMB_RS23750 0.72 -2.2 1,933,774 + AMB_RS23750 0.72 -0.8 1,933,774 + AMB_RS23750 0.72 +0.5 1,933,775 - AMB_RS23750 0.73 -2.2 1,933,775 - AMB_RS23750 0.73 +0.4 1,933,775 - AMB_RS23750 0.73 -0.2 1,933,775 - AMB_RS23750 0.73 -0.6 1,933,789 - AMB_RS23750 0.85 -1.6 1,933,789 - AMB_RS23750 0.85 +0.4 1,933,879 - -0.6 1,934,046 + AMB_RS09080 0.18 -1.3 1,934,047 - AMB_RS09080 0.18 -1.8 1,934,085 + AMB_RS09080 0.20 -0.2 1,934,085 + AMB_RS09080 0.20 -1.6 1,934,086 - AMB_RS09080 0.21 -1.2 1,934,091 + AMB_RS09080 0.21 -0.1 1,934,091 + AMB_RS09080 0.21 -1.2 1,934,092 - AMB_RS09080 0.21 -0.1 1,934,092 - AMB_RS09080 0.21 +0.6 1,934,092 - AMB_RS09080 0.21 -1.9 1,934,126 + AMB_RS09080 0.23 -0.1 1,934,126 + AMB_RS09080 0.23 -1.4 1,934,126 + AMB_RS09080 0.23 -1.4 1,934,126 + AMB_RS09080 0.23 -0.3 1,934,126 + AMB_RS09080 0.23 -0.4 1,934,126 + AMB_RS09080 0.23 +0.4 1,934,126 + AMB_RS09080 0.23 +0.1 1,934,126 + AMB_RS09080 0.23 +0.2 1,934,126 + AMB_RS09080 0.23 -0.5 1,934,127 - AMB_RS09080 0.23 -1.9 1,934,127 - AMB_RS09080 0.23 -0.6 1,934,173 - AMB_RS09080 0.27 -1.9 1,934,274 + AMB_RS09080 0.34 -2.1 1,934,274 + AMB_RS09080 0.34 -1.6 1,934,478 + AMB_RS09080 0.49 +0.3 1,934,479 - AMB_RS09080 0.49 -0.2 1,934,479 - AMB_RS09080 0.49 -0.5 1,934,652 + AMB_RS09080 0.62 +0.1 1,934,652 + AMB_RS09080 0.62 -1.6 1,934,652 + AMB_RS09080 0.62 +0.4 1,934,653 - AMB_RS09080 0.62 -2.3 1,934,653 - AMB_RS09080 0.62 -3.1 1,934,653 - AMB_RS09080 0.62 +1.7
Or see this region's nucleotide sequence