Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22755 and AMB_RS22760 are separated by 146 nucleotides AMB_RS22760 and AMB_RS22765 overlap by 4 nucleotides AMB_RS22765 and AMB_RS22770 are separated by 85 nucleotides
AMB_RS22755: AMB_RS22755 - heat-inducible transcriptional repressor HrcA, at 4,911,891 to 4,912,955
_RS22755
AMB_RS22760: AMB_RS22760 - ribonuclease PH, at 4,913,102 to 4,913,821
_RS22760
AMB_RS22765: AMB_RS22765 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 4,913,818 to 4,914,423
_RS22765
AMB_RS22770: AMB_RS22770 - radical SAM protein, at 4,914,509 to 4,915,636
_RS22770
Position (kb)
4913
4914
4915 Strain fitness (log2 ratio)
-2
-1
0
1 at 4912.877 kb on - strand at 4912.877 kb on - strand at 4912.877 kb on - strand at 4912.877 kb on - strand at 4912.877 kb on - strand at 4913.052 kb on + strand at 4913.060 kb on + strand at 4913.060 kb on + strand at 4913.060 kb on + strand at 4913.061 kb on - strand at 4913.061 kb on - strand at 4913.085 kb on + strand at 4913.600 kb on + strand, within AMB_RS22760 at 4913.600 kb on + strand, within AMB_RS22760 at 4914.088 kb on + strand, within AMB_RS22765 at 4914.292 kb on + strand, within AMB_RS22765 at 4914.533 kb on + strand at 4914.533 kb on + strand at 4914.534 kb on - strand at 4914.534 kb on - strand at 4914.534 kb on - strand at 4914.570 kb on - strand at 4915.271 kb on + strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 4,912,877 - -1.7 4,912,877 - +0.7 4,912,877 - -0.9 4,912,877 - -1.1 4,912,877 - -1.1 4,913,052 + -2.0 4,913,060 + -0.1 4,913,060 + -1.5 4,913,060 + +0.4 4,913,061 - -1.1 4,913,061 - +1.3 4,913,085 + -0.1 4,913,600 + AMB_RS22760 0.69 -0.9 4,913,600 + AMB_RS22760 0.69 -2.1 4,914,088 + AMB_RS22765 0.45 -0.1 4,914,292 + AMB_RS22765 0.78 -0.7 4,914,533 + -0.1 4,914,533 + -0.3 4,914,534 - +0.1 4,914,534 - -0.9 4,914,534 - -0.7 4,914,570 - -0.4 4,915,271 + AMB_RS22770 0.68 -0.1 4,915,272 - AMB_RS22770 0.68 -1.8 4,915,272 - AMB_RS22770 0.68 -1.8 4,915,272 - AMB_RS22770 0.68 +0.1
Or see this region's nucleotide sequence