Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20630 and AMB_RS20635 are separated by 1 nucleotides AMB_RS20635 and AMB_RS20640 are separated by 20 nucleotides AMB_RS20640 and AMB_RS20645 overlap by 4 nucleotides AMB_RS20645 and AMB_RS23800 are separated by 71 nucleotides
AMB_RS20630: AMB_RS20630 - glutamate 5-kinase, at 4,482,649 to 4,483,761
_RS20630
AMB_RS20635: AMB_RS20635 - N-acetylmuramoyl-L-alanine amidase, at 4,483,763 to 4,484,479
_RS20635
AMB_RS20640: AMB_RS20640 - glutamate-5-semialdehyde dehydrogenase, at 4,484,500 to 4,485,768
_RS20640
AMB_RS20645: AMB_RS20645 - nicotinate-nucleotide adenylyltransferase, at 4,485,765 to 4,486,376
_RS20645
AMB_RS23800: AMB_RS23800 - ribosome silencing factor, at 4,486,448 to 4,486,804
_RS23800
Position (kb) 4484
4485
4486 Strain fitness (log2 ratio) -2
-1
0
1 at 4484.078 kb on + strand, within AMB_RS20635 at 4484.078 kb on + strand, within AMB_RS20635 at 4484.078 kb on + strand, within AMB_RS20635 at 4484.078 kb on + strand, within AMB_RS20635 at 4484.078 kb on + strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.079 kb on - strand, within AMB_RS20635 at 4484.303 kb on + strand, within AMB_RS20635 at 4484.303 kb on + strand, within AMB_RS20635 at 4484.309 kb on + strand, within AMB_RS20635 at 4484.310 kb on - strand, within AMB_RS20635 at 4485.755 kb on + strand at 4486.375 kb on - strand at 4486.375 kb on - strand at 4486.375 kb on - strand at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.713 kb on - strand, within AMB_RS23800 at 4486.713 kb on - strand, within AMB_RS23800
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 4,484,078 + AMB_RS20635 0.44 +1.5 4,484,078 + AMB_RS20635 0.44 -1.1 4,484,078 + AMB_RS20635 0.44 +1.2 4,484,078 + AMB_RS20635 0.44 +0.1 4,484,078 + AMB_RS20635 0.44 -0.2 4,484,079 - AMB_RS20635 0.44 -1.5 4,484,079 - AMB_RS20635 0.44 -1.1 4,484,079 - AMB_RS20635 0.44 +0.5 4,484,079 - AMB_RS20635 0.44 -0.9 4,484,079 - AMB_RS20635 0.44 -0.0 4,484,079 - AMB_RS20635 0.44 -0.0 4,484,303 + AMB_RS20635 0.75 -0.8 4,484,303 + AMB_RS20635 0.75 -1.3 4,484,309 + AMB_RS20635 0.76 +0.1 4,484,310 - AMB_RS20635 0.76 +0.1 4,485,755 + -1.9 4,486,375 - -1.9 4,486,375 - +0.2 4,486,375 - -0.5 4,486,712 + AMB_RS23800 0.74 -2.2 4,486,712 + AMB_RS23800 0.74 -0.5 4,486,712 + AMB_RS23800 0.74 -1.1 4,486,712 + AMB_RS23800 0.74 -0.2 4,486,713 - AMB_RS23800 0.74 +0.2 4,486,713 - AMB_RS23800 0.74 -0.1
Or see this region's nucleotide sequence