Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18915 and AMB_RS18920 are separated by 292 nucleotides AMB_RS18920 and AMB_RS18925 are separated by 258 nucleotides AMB_RS18925 and AMB_RS18930 are separated by 18 nucleotides AMB_RS18930 and AMB_RS18935 overlap by 4 nucleotides
AMB_RS18915: AMB_RS18915 - Flp family type IVb pilin, at 4,113,627 to 4,113,788
_RS18915
AMB_RS18920: AMB_RS18920 - Flp family type IVb pilin, at 4,114,081 to 4,114,275
_RS18920
AMB_RS18925: AMB_RS18925 - type 4 prepilin peptidase 1, at 4,114,534 to 4,115,016
_RS18925
AMB_RS18930: AMB_RS18930 - Flp pilus assembly protein CpaB, at 4,115,035 to 4,115,937
_RS18930
AMB_RS18935: AMB_RS18935 - Flp pilus assembly protein, secretin CpaC, at 4,115,934 to 4,117,421
_RS18935
Position (kb) 4114
4115
4116 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4113.537 kb on + strand at 4113.685 kb on - strand, within AMB_RS18915 at 4113.770 kb on + strand, within AMB_RS18915 at 4113.771 kb on - strand, within AMB_RS18915 at 4113.786 kb on + strand at 4113.786 kb on + strand at 4113.787 kb on - strand at 4113.787 kb on - strand at 4113.787 kb on - strand at 4114.442 kb on - strand at 4115.227 kb on + strand, within AMB_RS18930
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 4,113,537 + -0.5 4,113,685 - AMB_RS18915 0.36 -0.1 4,113,770 + AMB_RS18915 0.88 -1.3 4,113,771 - AMB_RS18915 0.89 -0.3 4,113,786 + -1.0 4,113,786 + +0.4 4,113,787 - -0.1 4,113,787 - -0.8 4,113,787 - -0.7 4,114,442 - -3.0 4,115,227 + AMB_RS18930 0.21 -0.0
Or see this region's nucleotide sequence