Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18140 and AMB_RS18145 are separated by 104 nucleotides AMB_RS18145 and AMB_RS18150 are separated by 220 nucleotides AMB_RS18150 and AMB_RS18155 are separated by 29 nucleotides
AMB_RS18140: AMB_RS18140 - malonyl-CoA synthase, at 3,935,179 to 3,936,705
_RS18140
AMB_RS18145: AMB_RS18145 - GntR family transcriptional regulator, at 3,936,810 to 3,937,469
_RS18145
AMB_RS18150: AMB_RS18150 - acyl-CoA dehydrogenase, at 3,937,690 to 3,938,826
_RS18150
AMB_RS18155: AMB_RS18155 - 3-hydroxyisobutyrate dehydrogenase, at 3,938,856 to 3,939,746
_RS18155
Position (kb)
3937
3938
3939 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3936.738 kb on + strand at 3936.751 kb on + strand at 3936.811 kb on + strand at 3936.812 kb on - strand at 3937.000 kb on + strand, within AMB_RS18145 at 3937.000 kb on + strand, within AMB_RS18145 at 3937.000 kb on + strand, within AMB_RS18145 at 3937.000 kb on + strand, within AMB_RS18145 at 3937.048 kb on + strand, within AMB_RS18145 at 3937.081 kb on + strand, within AMB_RS18145 at 3937.081 kb on + strand, within AMB_RS18145 at 3937.082 kb on - strand, within AMB_RS18145 at 3937.082 kb on - strand, within AMB_RS18145 at 3937.084 kb on + strand, within AMB_RS18145 at 3937.298 kb on - strand, within AMB_RS18145 at 3937.504 kb on + strand at 3937.507 kb on - strand at 3937.680 kb on + strand at 3937.681 kb on - strand at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.849 kb on + strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.850 kb on - strand, within AMB_RS18150 at 3937.945 kb on + strand, within AMB_RS18150 at 3937.945 kb on + strand, within AMB_RS18150 at 3937.946 kb on - strand, within AMB_RS18150 at 3937.946 kb on - strand, within AMB_RS18150 at 3937.946 kb on - strand, within AMB_RS18150 at 3937.947 kb on + strand, within AMB_RS18150 at 3937.947 kb on + strand, within AMB_RS18150 at 3937.947 kb on + strand, within AMB_RS18150 at 3937.947 kb on + strand, within AMB_RS18150 at 3937.948 kb on - strand, within AMB_RS18150 at 3937.948 kb on - strand, within AMB_RS18150 at 3937.948 kb on - strand, within AMB_RS18150 at 3938.053 kb on + strand, within AMB_RS18150 at 3938.054 kb on - strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.128 kb on + strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.129 kb on - strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.182 kb on + strand, within AMB_RS18150 at 3938.183 kb on - strand, within AMB_RS18150 at 3938.183 kb on - strand, within AMB_RS18150 at 3938.183 kb on - strand, within AMB_RS18150 at 3938.418 kb on + strand, within AMB_RS18150 at 3938.713 kb on + strand at 3938.713 kb on + strand at 3938.713 kb on + strand at 3938.713 kb on + strand at 3938.725 kb on + strand at 3938.725 kb on + strand at 3938.726 kb on - strand at 3938.726 kb on - strand at 3938.741 kb on - strand at 3938.824 kb on + strand at 3938.824 kb on + strand at 3938.824 kb on + strand at 3938.825 kb on - strand at 3938.825 kb on - strand at 3938.825 kb on - strand at 3938.825 kb on - strand at 3938.834 kb on + strand at 3938.835 kb on - strand at 3939.510 kb on + strand, within AMB_RS18155 at 3939.510 kb on + strand, within AMB_RS18155 at 3939.511 kb on - strand, within AMB_RS18155 at 3939.511 kb on - strand, within AMB_RS18155 at 3939.511 kb on - strand, within AMB_RS18155 at 3939.511 kb on - strand, within AMB_RS18155 at 3939.511 kb on - strand, within AMB_RS18155 at 3939.558 kb on + strand, within AMB_RS18155 at 3939.558 kb on + strand, within AMB_RS18155 at 3939.558 kb on + strand, within AMB_RS18155 at 3939.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,936,738 + -0.8 3,936,751 + -0.5 3,936,811 + +0.4 3,936,812 - -1.3 3,937,000 + AMB_RS18145 0.29 -2.4 3,937,000 + AMB_RS18145 0.29 -0.5 3,937,000 + AMB_RS18145 0.29 -2.8 3,937,000 + AMB_RS18145 0.29 -0.4 3,937,048 + AMB_RS18145 0.36 -1.8 3,937,081 + AMB_RS18145 0.41 -1.3 3,937,081 + AMB_RS18145 0.41 -0.1 3,937,082 - AMB_RS18145 0.41 -0.2 3,937,082 - AMB_RS18145 0.41 +0.1 3,937,084 + AMB_RS18145 0.42 -0.6 3,937,298 - AMB_RS18145 0.74 +0.5 3,937,504 + -0.7 3,937,507 - -0.1 3,937,680 + +0.0 3,937,681 - +1.4 3,937,849 + AMB_RS18150 0.14 -4.6 3,937,849 + AMB_RS18150 0.14 -0.8 3,937,849 + AMB_RS18150 0.14 -1.3 3,937,849 + AMB_RS18150 0.14 -0.0 3,937,849 + AMB_RS18150 0.14 -0.4 3,937,849 + AMB_RS18150 0.14 +0.1 3,937,849 + AMB_RS18150 0.14 +1.5 3,937,850 - AMB_RS18150 0.14 -0.5 3,937,850 - AMB_RS18150 0.14 +0.0 3,937,850 - AMB_RS18150 0.14 +0.2 3,937,850 - AMB_RS18150 0.14 +0.3 3,937,850 - AMB_RS18150 0.14 -0.9 3,937,850 - AMB_RS18150 0.14 -0.5 3,937,850 - AMB_RS18150 0.14 +0.5 3,937,945 + AMB_RS18150 0.22 -0.1 3,937,945 + AMB_RS18150 0.22 -0.4 3,937,946 - AMB_RS18150 0.23 +0.7 3,937,946 - AMB_RS18150 0.23 +0.2 3,937,946 - AMB_RS18150 0.23 +0.3 3,937,947 + AMB_RS18150 0.23 +0.0 3,937,947 + AMB_RS18150 0.23 -0.1 3,937,947 + AMB_RS18150 0.23 -0.0 3,937,947 + AMB_RS18150 0.23 -1.1 3,937,948 - AMB_RS18150 0.23 +0.1 3,937,948 - AMB_RS18150 0.23 -0.8 3,937,948 - AMB_RS18150 0.23 +0.0 3,938,053 + AMB_RS18150 0.32 -0.7 3,938,054 - AMB_RS18150 0.32 -0.5 3,938,128 + AMB_RS18150 0.39 +0.5 3,938,128 + AMB_RS18150 0.39 -1.5 3,938,128 + AMB_RS18150 0.39 -1.3 3,938,128 + AMB_RS18150 0.39 -1.5 3,938,128 + AMB_RS18150 0.39 +0.5 3,938,128 + AMB_RS18150 0.39 -0.6 3,938,128 + AMB_RS18150 0.39 -0.5 3,938,128 + AMB_RS18150 0.39 -0.8 3,938,128 + AMB_RS18150 0.39 +0.5 3,938,129 - AMB_RS18150 0.39 +0.3 3,938,129 - AMB_RS18150 0.39 +0.6 3,938,129 - AMB_RS18150 0.39 +0.5 3,938,129 - AMB_RS18150 0.39 -1.7 3,938,129 - AMB_RS18150 0.39 +1.0 3,938,129 - AMB_RS18150 0.39 +0.2 3,938,129 - AMB_RS18150 0.39 -0.8 3,938,182 + AMB_RS18150 0.43 +0.3 3,938,182 + AMB_RS18150 0.43 -0.8 3,938,182 + AMB_RS18150 0.43 +0.2 3,938,182 + AMB_RS18150 0.43 +0.2 3,938,182 + AMB_RS18150 0.43 -0.5 3,938,182 + AMB_RS18150 0.43 +0.3 3,938,183 - AMB_RS18150 0.43 -0.5 3,938,183 - AMB_RS18150 0.43 +0.2 3,938,183 - AMB_RS18150 0.43 -1.3 3,938,418 + AMB_RS18150 0.64 +0.5 3,938,713 + +1.5 3,938,713 + +0.0 3,938,713 + -0.1 3,938,713 + -0.3 3,938,725 + +0.1 3,938,725 + +0.3 3,938,726 - +0.5 3,938,726 - -0.3 3,938,741 - +0.1 3,938,824 + +0.5 3,938,824 + +0.4 3,938,824 + -0.4 3,938,825 - -0.2 3,938,825 - -0.6 3,938,825 - -0.5 3,938,825 - +0.2 3,938,834 + +0.9 3,938,835 - -0.3 3,939,510 + AMB_RS18155 0.73 +0.6 3,939,510 + AMB_RS18155 0.73 -0.5 3,939,511 - AMB_RS18155 0.74 +0.1 3,939,511 - AMB_RS18155 0.74 -0.5 3,939,511 - AMB_RS18155 0.74 +0.1 3,939,511 - AMB_RS18155 0.74 -0.2 3,939,511 - AMB_RS18155 0.74 -0.5 3,939,558 + AMB_RS18155 0.79 -0.6 3,939,558 + AMB_RS18155 0.79 -0.6 3,939,558 + AMB_RS18155 0.79 +0.1 3,939,669 + -0.0
Or see this region's nucleotide sequence