Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17325 and AMB_RS17330 are separated by 21 nucleotides AMB_RS17330 and AMB_RS17335 are separated by 60 nucleotides AMB_RS17335 and AMB_RS17340 are separated by 3 nucleotides AMB_RS17340 and AMB_RS17345 are separated by 45 nucleotides AMB_RS17345 and AMB_RS17350 are separated by 23 nucleotides
AMB_RS17325: AMB_RS17325 - multidrug efflux RND transporter permease subunit, at 3,751,463 to 3,754,642
_RS17325
AMB_RS17330: AMB_RS17330 - cytochrome c, at 3,754,664 to 3,755,020
_RS17330
AMB_RS17335: AMB_RS17335 - N-acyl-L-homoserine lactone synthetase, at 3,755,081 to 3,756,244
_RS17335
AMB_RS17340: AMB_RS17340 - rubredoxin, at 3,756,248 to 3,756,412
_RS17340
AMB_RS17345: AMB_RS17345 - rubredoxin, at 3,756,458 to 3,756,661
_RS17345
AMB_RS17350: AMB_RS17350 - rubrerythrin, at 3,756,685 to 3,757,161
_RS17350
Position (kb)
3755
3756
3757 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3754.144 kb on - strand, within AMB_RS17325 at 3754.144 kb on - strand, within AMB_RS17325 at 3754.160 kb on + strand, within AMB_RS17325 at 3754.160 kb on + strand, within AMB_RS17325 at 3754.161 kb on - strand, within AMB_RS17325 at 3754.161 kb on - strand, within AMB_RS17325 at 3754.508 kb on + strand at 3754.509 kb on - strand at 3754.509 kb on - strand at 3754.559 kb on + strand at 3754.559 kb on + strand at 3754.560 kb on - strand at 3754.640 kb on + strand at 3754.640 kb on + strand at 3754.666 kb on - strand at 3754.683 kb on + strand at 3754.774 kb on - strand, within AMB_RS17330 at 3754.774 kb on - strand, within AMB_RS17330 at 3754.774 kb on - strand, within AMB_RS17330 at 3754.831 kb on - strand, within AMB_RS17330 at 3754.831 kb on - strand, within AMB_RS17330 at 3755.035 kb on + strand at 3755.035 kb on + strand at 3755.038 kb on + strand at 3755.038 kb on + strand at 3755.038 kb on + strand at 3755.039 kb on - strand at 3755.039 kb on - strand at 3755.039 kb on - strand at 3755.039 kb on - strand at 3755.039 kb on - strand at 3755.039 kb on - strand at 3755.874 kb on + strand, within AMB_RS17335 at 3755.874 kb on + strand, within AMB_RS17335 at 3755.875 kb on - strand, within AMB_RS17335 at 3755.916 kb on + strand, within AMB_RS17335 at 3756.108 kb on + strand, within AMB_RS17335 at 3756.184 kb on + strand at 3756.184 kb on + strand at 3756.185 kb on - strand at 3756.185 kb on - strand at 3756.185 kb on - strand at 3756.185 kb on - strand at 3756.185 kb on - strand at 3756.186 kb on + strand at 3756.211 kb on + strand at 3756.402 kb on + strand at 3756.402 kb on + strand at 3756.402 kb on + strand at 3756.615 kb on + strand, within AMB_RS17345 at 3756.615 kb on + strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.891 kb on - strand, within AMB_RS17350 at 3756.902 kb on + strand, within AMB_RS17350 at 3756.902 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.909 kb on - strand, within AMB_RS17350 at 3756.909 kb on - strand, within AMB_RS17350 at 3757.082 kb on + strand, within AMB_RS17350 at 3757.082 kb on + strand, within AMB_RS17350
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,754,144 - AMB_RS17325 0.84 -0.5 3,754,144 - AMB_RS17325 0.84 -0.1 3,754,160 + AMB_RS17325 0.85 -1.7 3,754,160 + AMB_RS17325 0.85 -0.7 3,754,161 - AMB_RS17325 0.85 +0.2 3,754,161 - AMB_RS17325 0.85 +0.7 3,754,508 + +0.1 3,754,509 - -1.3 3,754,509 - +1.2 3,754,559 + -0.2 3,754,559 + +0.3 3,754,560 - -0.1 3,754,640 + -1.3 3,754,640 + -0.5 3,754,666 - +0.5 3,754,683 + -0.9 3,754,774 - AMB_RS17330 0.31 -1.7 3,754,774 - AMB_RS17330 0.31 -1.3 3,754,774 - AMB_RS17330 0.31 +0.4 3,754,831 - AMB_RS17330 0.47 +0.2 3,754,831 - AMB_RS17330 0.47 -2.8 3,755,035 + -2.1 3,755,035 + -0.4 3,755,038 + -1.3 3,755,038 + -1.3 3,755,038 + -0.5 3,755,039 - -0.4 3,755,039 - -0.6 3,755,039 - -1.2 3,755,039 - -0.5 3,755,039 - -0.3 3,755,039 - +0.8 3,755,874 + AMB_RS17335 0.68 -3.3 3,755,874 + AMB_RS17335 0.68 -3.1 3,755,875 - AMB_RS17335 0.68 -4.8 3,755,916 + AMB_RS17335 0.72 -1.9 3,756,108 + AMB_RS17335 0.88 -1.1 3,756,184 + -1.3 3,756,184 + -3.0 3,756,185 - -1.1 3,756,185 - -1.3 3,756,185 - -0.9 3,756,185 - -0.4 3,756,185 - -2.9 3,756,186 + -4.6 3,756,211 + -1.7 3,756,402 + -1.7 3,756,402 + -1.7 3,756,402 + -3.9 3,756,615 + AMB_RS17345 0.77 -2.8 3,756,615 + AMB_RS17345 0.77 -4.3 3,756,616 - AMB_RS17345 0.77 -2.2 3,756,616 - AMB_RS17345 0.77 +0.4 3,756,616 - AMB_RS17345 0.77 -0.4 3,756,891 - AMB_RS17350 0.43 -2.5 3,756,902 + AMB_RS17350 0.45 -5.8 3,756,902 + AMB_RS17350 0.45 -3.3 3,756,908 + AMB_RS17350 0.47 -1.8 3,756,908 + AMB_RS17350 0.47 -3.7 3,756,908 + AMB_RS17350 0.47 -1.9 3,756,909 - AMB_RS17350 0.47 -1.9 3,756,909 - AMB_RS17350 0.47 -2.1 3,757,082 + AMB_RS17350 0.83 -2.1 3,757,082 + AMB_RS17350 0.83 -2.2
Or see this region's nucleotide sequence