Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16870 and AMB_RS16875 are separated by 373 nucleotides AMB_RS16875 and AMB_RS23785 are separated by 79 nucleotides AMB_RS23785 and AMB_RS16880 overlap by 4 nucleotides AMB_RS16880 and AMB_RS16885 overlap by 4 nucleotides AMB_RS16885 and AMB_RS16890 are separated by 4 nucleotides AMB_RS16890 and AMB_RS16895 are separated by 88 nucleotides
AMB_RS16870: AMB_RS16870 - ferric reductase, at 3,627,242 to 3,628,576
_RS16870
AMB_RS16875: AMB_RS16875 - tRNA-Phe, at 3,628,950 to 3,629,025
_RS16875
AMB_RS23785: AMB_RS23785 - DNA gyrase inhibitor YacG, at 3,629,105 to 3,629,275
_RS23785
AMB_RS16880: AMB_RS16880 - ribonuclease G, at 3,629,272 to 3,630,306
_RS16880
AMB_RS16885: AMB_RS16885 - septum formation inhibitor Maf, at 3,630,303 to 3,630,875
_RS16885
AMB_RS16890: AMB_RS16890 - translation initiation factor IF-1, at 3,630,880 to 3,631,098
_RS16890
AMB_RS16895: AMB_RS16895 - arsenate reductase ArsC, at 3,631,187 to 3,631,630
_RS16895
Position (kb)
3629
3630
3631 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3628.421 kb on - strand, within AMB_RS16870 at 3628.452 kb on + strand at 3628.453 kb on - strand at 3628.453 kb on - strand at 3628.482 kb on + strand at 3628.507 kb on + strand at 3628.541 kb on + strand at 3628.627 kb on + strand at 3628.627 kb on + strand at 3628.629 kb on + strand at 3628.629 kb on + strand at 3628.630 kb on - strand at 3628.630 kb on - strand at 3628.630 kb on - strand at 3628.659 kb on - strand at 3628.673 kb on - strand at 3628.707 kb on + strand at 3628.708 kb on - strand at 3628.708 kb on - strand at 3628.751 kb on + strand at 3628.814 kb on + strand at 3628.925 kb on - strand at 3629.155 kb on - strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.217 kb on + strand, within AMB_RS23785 at 3629.218 kb on - strand, within AMB_RS23785 at 3629.218 kb on - strand, within AMB_RS23785 at 3629.218 kb on - strand, within AMB_RS23785 at 3629.218 kb on - strand, within AMB_RS23785 at 3629.218 kb on - strand, within AMB_RS23785 at 3630.374 kb on - strand, within AMB_RS16885 at 3630.398 kb on - strand, within AMB_RS16885 at 3630.464 kb on - strand, within AMB_RS16885 at 3630.882 kb on - strand at 3630.882 kb on - strand at 3630.882 kb on - strand at 3631.186 kb on - strand at 3631.189 kb on - strand at 3631.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,628,421 - AMB_RS16870 0.88 -0.1 3,628,452 + +0.5 3,628,453 - -1.4 3,628,453 - +0.2 3,628,482 + -0.0 3,628,507 + -0.5 3,628,541 + -0.1 3,628,627 + +0.5 3,628,627 + -0.5 3,628,629 + -0.3 3,628,629 + -2.8 3,628,630 - -0.4 3,628,630 - -2.1 3,628,630 - -0.1 3,628,659 - -0.3 3,628,673 - -1.4 3,628,707 + -0.0 3,628,708 - +0.1 3,628,708 - -0.5 3,628,751 + +0.5 3,628,814 + -1.7 3,628,925 - +0.0 3,629,155 - AMB_RS23785 0.29 -0.5 3,629,217 + AMB_RS23785 0.65 -0.1 3,629,217 + AMB_RS23785 0.65 -0.5 3,629,217 + AMB_RS23785 0.65 -1.5 3,629,217 + AMB_RS23785 0.65 -0.1 3,629,217 + AMB_RS23785 0.65 +0.7 3,629,217 + AMB_RS23785 0.65 -1.7 3,629,217 + AMB_RS23785 0.65 +0.4 3,629,217 + AMB_RS23785 0.65 -0.3 3,629,218 - AMB_RS23785 0.66 -1.9 3,629,218 - AMB_RS23785 0.66 +0.1 3,629,218 - AMB_RS23785 0.66 -0.9 3,629,218 - AMB_RS23785 0.66 +0.2 3,629,218 - AMB_RS23785 0.66 -0.5 3,630,374 - AMB_RS16885 0.12 -0.3 3,630,398 - AMB_RS16885 0.17 -0.6 3,630,464 - AMB_RS16885 0.28 -0.1 3,630,882 - +0.1 3,630,882 - -1.4 3,630,882 - +1.4 3,631,186 - +0.9 3,631,189 - -0.5 3,631,189 - -0.9
Or see this region's nucleotide sequence