Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16210 and AMB_RS16215 are separated by 116 nucleotides AMB_RS16215 and AMB_RS16220 are separated by 167 nucleotides AMB_RS16220 and AMB_RS16225 overlap by 4 nucleotides AMB_RS16225 and AMB_RS16230 are separated by 9 nucleotides
AMB_RS16210: AMB_RS16210 - peptidoglycan-associated lipoprotein Pal, at 3,477,339 to 3,477,827
_RS16210
AMB_RS16215: AMB_RS16215 - peptidoglycan-associated lipoprotein Pal, at 3,477,944 to 3,478,429
_RS16215
AMB_RS16220: AMB_RS16220 - protein TolB, at 3,478,597 to 3,479,940
_RS16220
AMB_RS16225: AMB_RS16225 - energy transducer TonB, at 3,479,937 to 3,480,878
_RS16225
AMB_RS16230: AMB_RS16230 - protein TolR, at 3,480,888 to 3,481,349
_RS16230
Position (kb) 3478
3479
3480 Strain fitness (log2 ratio) -1
0
1 at 3477.668 kb on + strand, within AMB_RS16210 at 3477.700 kb on + strand, within AMB_RS16210 at 3477.701 kb on - strand, within AMB_RS16210 at 3477.701 kb on - strand, within AMB_RS16210 at 3477.701 kb on - strand, within AMB_RS16210 at 3477.701 kb on - strand, within AMB_RS16210 at 3477.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,477,668 + AMB_RS16210 0.67 -1.4 3,477,700 + AMB_RS16210 0.74 -0.1 3,477,701 - AMB_RS16210 0.74 -1.4 3,477,701 - AMB_RS16210 0.74 -1.6 3,477,701 - AMB_RS16210 0.74 -0.3 3,477,701 - AMB_RS16210 0.74 +0.1 3,477,810 - +0.3
Or see this region's nucleotide sequence