Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14830 and AMB_RS14835 are separated by 75 nucleotides AMB_RS14835 and AMB_RS14840 are separated by 487 nucleotides AMB_RS14840 and AMB_RS14845 are separated by 67 nucleotides AMB_RS14845 and AMB_RS14850 are separated by 78 nucleotides
AMB_RS14830: AMB_RS14830 - Crp/Fnr family transcriptional regulator, at 3,149,519 to 3,150,388
_RS14830
AMB_RS14835: AMB_RS14835 - hypothetical protein, at 3,150,464 to 3,150,871
_RS14835
AMB_RS14840: AMB_RS14840 - DUF2478 domain-containing protein, at 3,151,359 to 3,151,946
_RS14840
AMB_RS14845: AMB_RS14845 - molybdenum-dependent transcriptional regulator, at 3,152,014 to 3,152,808
_RS14845
AMB_RS14850: AMB_RS14850 - molybdenum-pterin-binding protein, at 3,152,887 to 3,153,090
_RS14850
Position (kb)
3151
3152 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3150.427 kb on + strand at 3150.428 kb on - strand at 3150.428 kb on - strand at 3150.428 kb on - strand at 3150.428 kb on - strand at 3150.428 kb on - strand at 3150.884 kb on - strand at 3151.058 kb on - strand at 3151.113 kb on + strand at 3151.147 kb on + strand at 3151.147 kb on + strand at 3151.147 kb on + strand at 3151.147 kb on + strand at 3151.148 kb on - strand at 3151.148 kb on - strand at 3151.149 kb on + strand at 3151.149 kb on + strand at 3151.149 kb on + strand at 3151.149 kb on + strand at 3151.150 kb on - strand at 3151.267 kb on + strand at 3151.276 kb on - strand at 3151.360 kb on + strand at 3151.360 kb on + strand at 3151.361 kb on - strand at 3151.733 kb on - strand, within AMB_RS14840 at 3151.733 kb on - strand, within AMB_RS14840 at 3151.933 kb on + strand at 3151.934 kb on - strand at 3151.934 kb on - strand at 3151.934 kb on - strand at 3151.946 kb on + strand at 3152.015 kb on + strand at 3152.015 kb on + strand at 3152.015 kb on + strand at 3152.016 kb on - strand at 3152.016 kb on - strand at 3152.016 kb on - strand at 3152.016 kb on - strand at 3152.199 kb on + strand, within AMB_RS14845 at 3152.666 kb on + strand, within AMB_RS14845 at 3152.666 kb on + strand, within AMB_RS14845 at 3152.667 kb on - strand, within AMB_RS14845 at 3152.667 kb on - strand, within AMB_RS14845 at 3152.667 kb on - strand, within AMB_RS14845 at 3152.667 kb on - strand, within AMB_RS14845 at 3152.779 kb on + strand at 3152.796 kb on + strand at 3152.829 kb on + strand at 3152.830 kb on - strand at 3152.830 kb on - strand at 3152.838 kb on + strand at 3152.838 kb on + strand at 3152.838 kb on + strand at 3152.838 kb on + strand at 3152.838 kb on + strand at 3152.839 kb on - strand at 3152.839 kb on - strand at 3152.841 kb on - strand at 3152.889 kb on - strand at 3152.889 kb on - strand at 3152.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,150,427 + +0.9 3,150,428 - -0.2 3,150,428 - +0.4 3,150,428 - -0.3 3,150,428 - -0.6 3,150,428 - -2.4 3,150,884 - -0.3 3,151,058 - +0.7 3,151,113 + -1.1 3,151,147 + -0.2 3,151,147 + -0.4 3,151,147 + +0.0 3,151,147 + +0.3 3,151,148 - +0.1 3,151,148 - +0.0 3,151,149 + -0.2 3,151,149 + +0.6 3,151,149 + +0.6 3,151,149 + +0.1 3,151,150 - -1.3 3,151,267 + +0.2 3,151,276 - -0.4 3,151,360 + -2.4 3,151,360 + +0.4 3,151,361 - -1.1 3,151,733 - AMB_RS14840 0.64 -1.0 3,151,733 - AMB_RS14840 0.64 +0.4 3,151,933 + +0.1 3,151,934 - +0.5 3,151,934 - +0.4 3,151,934 - -1.9 3,151,946 + -0.6 3,152,015 + -0.1 3,152,015 + -0.6 3,152,015 + +0.1 3,152,016 - +0.5 3,152,016 - +1.1 3,152,016 - -0.1 3,152,016 - +0.2 3,152,199 + AMB_RS14845 0.23 -0.5 3,152,666 + AMB_RS14845 0.82 -0.3 3,152,666 + AMB_RS14845 0.82 -1.4 3,152,667 - AMB_RS14845 0.82 +1.4 3,152,667 - AMB_RS14845 0.82 -0.9 3,152,667 - AMB_RS14845 0.82 -1.7 3,152,667 - AMB_RS14845 0.82 +0.3 3,152,779 + -0.9 3,152,796 + -0.2 3,152,829 + +0.4 3,152,830 - +0.2 3,152,830 - +0.0 3,152,838 + +0.4 3,152,838 + +0.5 3,152,838 + -3.3 3,152,838 + -0.0 3,152,838 + -1.7 3,152,839 - -0.2 3,152,839 - +0.2 3,152,841 - -0.0 3,152,889 - +0.0 3,152,889 - -2.4 3,152,889 - -0.1
Or see this region's nucleotide sequence