Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14625 and AMB_RS14630 are separated by 234 nucleotides AMB_RS14630 and AMB_RS14635 are separated by 174 nucleotides AMB_RS14635 and AMB_RS14640 are separated by 358 nucleotides AMB_RS14640 and AMB_RS14645 are separated by 56 nucleotides
AMB_RS14625: AMB_RS14625 - MBL fold metallo-hydrolase, at 3,103,075 to 3,104,104
_RS14625
AMB_RS14630: AMB_RS14630 - hypothetical protein, at 3,104,339 to 3,104,740
_RS14630
AMB_RS14635: AMB_RS14635 - hypothetical protein, at 3,104,915 to 3,105,160
_RS14635
AMB_RS14640: AMB_RS14640 - nitrous oxide reductase accessory protein NosL, at 3,105,519 to 3,106,007
_RS14640
AMB_RS14645: AMB_RS14645 - sulfite exporter TauE/SafE family protein, at 3,106,064 to 3,106,753
_RS14645
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3104.189 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.191 kb on + strand at 3104.192 kb on - strand at 3104.192 kb on - strand at 3104.192 kb on - strand at 3104.192 kb on - strand at 3104.192 kb on - strand at 3104.192 kb on - strand at 3104.334 kb on + strand at 3104.334 kb on + strand at 3104.459 kb on + strand, within AMB_RS14630 at 3104.460 kb on - strand, within AMB_RS14630 at 3104.460 kb on - strand, within AMB_RS14630 at 3104.460 kb on - strand, within AMB_RS14630 at 3104.460 kb on - strand, within AMB_RS14630 at 3104.460 kb on - strand, within AMB_RS14630 at 3104.610 kb on - strand, within AMB_RS14630 at 3104.813 kb on + strand at 3104.813 kb on + strand at 3104.814 kb on - strand at 3104.814 kb on - strand at 3104.814 kb on - strand at 3104.963 kb on + strand, within AMB_RS14635 at 3104.963 kb on + strand, within AMB_RS14635 at 3104.964 kb on - strand, within AMB_RS14635 at 3104.967 kb on + strand, within AMB_RS14635 at 3104.968 kb on - strand, within AMB_RS14635 at 3105.128 kb on - strand, within AMB_RS14635 at 3105.142 kb on + strand at 3105.142 kb on + strand at 3105.143 kb on - strand at 3105.143 kb on - strand at 3105.158 kb on + strand at 3105.159 kb on - strand at 3105.509 kb on + strand at 3105.510 kb on - strand at 3105.520 kb on + strand at 3105.520 kb on + strand at 3105.536 kb on + strand at 3105.669 kb on + strand, within AMB_RS14640 at 3105.669 kb on + strand, within AMB_RS14640 at 3105.669 kb on + strand, within AMB_RS14640 at 3105.670 kb on - strand, within AMB_RS14640 at 3105.670 kb on - strand, within AMB_RS14640 at 3105.670 kb on - strand, within AMB_RS14640 at 3105.725 kb on - strand, within AMB_RS14640 at 3105.727 kb on + strand, within AMB_RS14640 at 3105.727 kb on + strand, within AMB_RS14640 at 3105.727 kb on + strand, within AMB_RS14640 at 3105.727 kb on + strand, within AMB_RS14640 at 3105.728 kb on - strand, within AMB_RS14640 at 3105.829 kb on + strand, within AMB_RS14640 at 3105.829 kb on + strand, within AMB_RS14640 at 3105.829 kb on + strand, within AMB_RS14640 at 3105.830 kb on - strand, within AMB_RS14640 at 3105.877 kb on + strand, within AMB_RS14640
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,104,189 + +0.1 3,104,191 + +0.2 3,104,191 + -0.1 3,104,191 + -0.1 3,104,191 + +0.1 3,104,191 + +0.4 3,104,191 + -1.4 3,104,191 + -1.4 3,104,191 + +0.0 3,104,192 - -2.3 3,104,192 - -0.2 3,104,192 - -1.6 3,104,192 - -0.1 3,104,192 - -0.6 3,104,192 - +0.0 3,104,334 + -0.2 3,104,334 + +0.7 3,104,459 + AMB_RS14630 0.30 +0.4 3,104,460 - AMB_RS14630 0.30 -2.0 3,104,460 - AMB_RS14630 0.30 +0.4 3,104,460 - AMB_RS14630 0.30 -0.6 3,104,460 - AMB_RS14630 0.30 +0.6 3,104,460 - AMB_RS14630 0.30 -2.4 3,104,610 - AMB_RS14630 0.67 -1.6 3,104,813 + -2.4 3,104,813 + +0.3 3,104,814 - -0.2 3,104,814 - -1.2 3,104,814 - +0.3 3,104,963 + AMB_RS14635 0.20 +1.0 3,104,963 + AMB_RS14635 0.20 -0.0 3,104,964 - AMB_RS14635 0.20 -0.8 3,104,967 + AMB_RS14635 0.21 -0.2 3,104,968 - AMB_RS14635 0.22 -2.1 3,105,128 - AMB_RS14635 0.87 -0.1 3,105,142 + +0.8 3,105,142 + -2.2 3,105,143 - -0.1 3,105,143 - -0.4 3,105,158 + -0.5 3,105,159 - +0.1 3,105,509 + -1.2 3,105,510 - -0.2 3,105,520 + -1.4 3,105,520 + -0.9 3,105,536 + +0.9 3,105,669 + AMB_RS14640 0.31 +1.4 3,105,669 + AMB_RS14640 0.31 -2.2 3,105,669 + AMB_RS14640 0.31 +0.4 3,105,670 - AMB_RS14640 0.31 +0.0 3,105,670 - AMB_RS14640 0.31 -1.6 3,105,670 - AMB_RS14640 0.31 +2.4 3,105,725 - AMB_RS14640 0.42 -2.3 3,105,727 + AMB_RS14640 0.43 -0.6 3,105,727 + AMB_RS14640 0.43 -0.2 3,105,727 + AMB_RS14640 0.43 +0.6 3,105,727 + AMB_RS14640 0.43 -0.2 3,105,728 - AMB_RS14640 0.43 +0.3 3,105,829 + AMB_RS14640 0.63 +0.1 3,105,829 + AMB_RS14640 0.63 +0.5 3,105,829 + AMB_RS14640 0.63 +0.4 3,105,830 - AMB_RS14640 0.64 -0.1 3,105,877 + AMB_RS14640 0.73 -2.6
Or see this region's nucleotide sequence