Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14570 and AMB_RS14575 are separated by 582 nucleotides AMB_RS14575 and AMB_RS14580 are separated by 157 nucleotides AMB_RS14580 and AMB_RS14585 are separated by 6 nucleotides AMB_RS14585 and AMB_RS14590 overlap by 22 nucleotides
AMB_RS14570: AMB_RS14570 - DNA-binding response regulator, at 3,093,414 to 3,094,091
_RS14570
AMB_RS14575: AMB_RS14575 - Hsp20/alpha crystallin family protein, at 3,094,674 to 3,095,159
_RS14575
AMB_RS14580: AMB_RS14580 - hypothetical protein, at 3,095,317 to 3,095,574
_RS14580
AMB_RS14585: AMB_RS14585 - SOS response-associated peptidase, at 3,095,581 to 3,096,144
_RS14585
AMB_RS14590: AMB_RS14590 - TlyA family rRNA (cytidine-2'-O)-methyltransferase, at 3,096,123 to 3,096,890
_RS14590
Position (kb)
3094
3095
3096 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3093.785 kb on - strand, within AMB_RS14570 at 3093.800 kb on - strand, within AMB_RS14570 at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.107 kb on - strand at 3094.125 kb on + strand at 3094.126 kb on - strand at 3094.126 kb on - strand at 3094.126 kb on - strand at 3094.126 kb on - strand at 3094.126 kb on - strand at 3094.342 kb on + strand at 3094.343 kb on - strand at 3094.343 kb on - strand at 3094.343 kb on - strand at 3094.500 kb on + strand at 3094.500 kb on + strand at 3094.501 kb on - strand at 3094.662 kb on + strand at 3094.662 kb on + strand at 3094.663 kb on - strand at 3094.709 kb on + strand at 3094.849 kb on + strand, within AMB_RS14575 at 3094.849 kb on + strand, within AMB_RS14575 at 3094.849 kb on + strand, within AMB_RS14575 at 3094.849 kb on + strand, within AMB_RS14575 at 3094.850 kb on - strand, within AMB_RS14575 at 3095.027 kb on + strand, within AMB_RS14575 at 3095.027 kb on + strand, within AMB_RS14575 at 3095.073 kb on + strand, within AMB_RS14575 at 3095.073 kb on + strand, within AMB_RS14575 at 3095.157 kb on + strand at 3095.157 kb on + strand at 3095.157 kb on + strand at 3095.157 kb on + strand at 3095.157 kb on + strand at 3095.158 kb on - strand at 3095.272 kb on + strand at 3095.272 kb on + strand at 3095.273 kb on - strand at 3095.285 kb on + strand at 3096.158 kb on - strand at 3096.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,093,785 - AMB_RS14570 0.55 -2.7 3,093,800 - AMB_RS14570 0.57 -2.4 3,094,107 - +0.3 3,094,107 - -0.9 3,094,107 - -4.0 3,094,107 - -1.4 3,094,107 - -1.2 3,094,107 - +0.3 3,094,107 - -0.8 3,094,107 - -1.9 3,094,125 + -2.4 3,094,126 - +0.3 3,094,126 - -1.5 3,094,126 - +0.4 3,094,126 - +0.6 3,094,126 - -1.4 3,094,342 + -1.7 3,094,343 - -1.4 3,094,343 - -0.8 3,094,343 - -0.4 3,094,500 + +0.2 3,094,500 + -0.2 3,094,501 - -0.9 3,094,662 + +0.4 3,094,662 + -0.9 3,094,663 - -1.8 3,094,709 + -0.2 3,094,849 + AMB_RS14575 0.36 +1.4 3,094,849 + AMB_RS14575 0.36 -1.5 3,094,849 + AMB_RS14575 0.36 -0.2 3,094,849 + AMB_RS14575 0.36 +0.1 3,094,850 - AMB_RS14575 0.36 -1.1 3,095,027 + AMB_RS14575 0.73 -0.9 3,095,027 + AMB_RS14575 0.73 +0.0 3,095,073 + AMB_RS14575 0.82 -3.1 3,095,073 + AMB_RS14575 0.82 -0.0 3,095,157 + -2.3 3,095,157 + -0.0 3,095,157 + -1.8 3,095,157 + +0.1 3,095,157 + -0.1 3,095,158 - -0.9 3,095,272 + -1.0 3,095,272 + -3.9 3,095,273 - -0.9 3,095,285 + -0.2 3,096,158 - +0.2 3,096,158 - -0.1
Or see this region's nucleotide sequence